Detailed information NTG0892520

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R1T44_RS02490
• Genome accession: NZ_CP136695
• Coordinates: 557415..558569 (+)
• Length: 384 a.a.
• NCBI ID: WP_288987299.1
• Uniprot ID: -
• Organism: Cobetia amphilecti strain CI.11.F.A3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 552415..563569

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R1T44_RS02470 (R1T44_02470)	-	553634..554215 (-)	582	WP_043333437.1	YggT family protein	-
R1T44_RS02475 (R1T44_02475)	proC	554365..555207 (-)	843	WP_043333440.1	pyrroline-5-carboxylate reductase	-
R1T44_RS02480 (R1T44_02480)	-	555296..556048 (-)	753	WP_288987296.1	YggS family pyridoxal phosphate-dependent enzyme	-
R1T44_RS02485 (R1T44_02485)	pilT	556244..557275 (+)	1032	WP_043333445.1	type IV pilus twitching motility protein PilT	Machinery gene
R1T44_RS02490 (R1T44_02490)	pilU	557415..558569 (+)	1155	WP_288987299.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R1T44_RS02495 (R1T44_02495)	-	558660..559808 (-)	1149	WP_317292502.1	anhydro-N-acetylmuramic acid kinase	-
R1T44_RS02500 (R1T44_02500)	tyrS	559964..561172 (+)	1209	WP_054556461.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 384 a.a.
• Molecular weight: 43053.36 Da
• Isoelectric Point: 6.4514

>NTDB_id=892520 R1T44_RS02490 WP_288987299.1 557415..558569(+) (pilU) [Cobetia amphilecti strain CI.11.F.A3]
MTPHEWLHELLQLMVSKGSSDLFISTGTPPQMKVNGRMVALGDKKLSVDQVRELVLAPMSDMQRERFEEEREANFAHSLP
GVGRFRISAFYQRSQMGMVIRRIQLSIPSLEELRLPEIIKGLSETKRGLVIFVGGTGAGKSTSLAAMIQHRNQTSSGHII
CIEDPIEYIHPHQRSIVTQREVGIDTESFEVALRNTLRQAPDVIMIGEIRSRETMEHALTFAETGHLCLATLHANNANQA
LDRIIHFFPEERHEQVWMDLSLNLKGIVAQQLLPHKSGNTTQRVPAIEVMLRSPLIVDLIRKGAVVEIKDVMKRSQQQGM
MTFDQSLYALHQQGLITEEVALAHADSANDLRLMIKFGDSNSAQEAQLDVLNAASRFSLQGDDD

Nucleotide

• Length: 1155 bp

>NTDB_id=892520 R1T44_RS02490 WP_288987299.1 557415..558569(+) (pilU) [Cobetia amphilecti strain CI.11.F.A3]
ATGACTCCTCATGAATGGCTGCATGAACTTCTGCAGTTGATGGTCAGCAAGGGCAGCTCGGACCTGTTCATCTCCACCGG
CACACCGCCGCAGATGAAGGTCAATGGCCGCATGGTGGCGCTGGGTGACAAGAAGCTGAGTGTCGATCAGGTGAGAGAGC
TGGTGCTCGCACCGATGAGTGACATGCAGCGCGAGCGCTTCGAGGAAGAACGCGAGGCCAACTTCGCGCATAGCTTGCCG
GGAGTCGGGCGCTTCAGGATCAGCGCGTTCTACCAGCGCAGCCAGATGGGCATGGTGATTCGCCGCATCCAGCTGTCGAT
TCCCAGCCTGGAAGAGTTGCGGCTGCCGGAGATCATCAAGGGGCTTTCCGAGACCAAGCGTGGTCTGGTGATCTTCGTCG
GCGGCACCGGCGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCCAGCACCGCAACCAGACCTCGAGCGGGCACATCATC
TGCATCGAAGACCCGATCGAGTATATCCACCCTCACCAGCGCTCCATCGTGACGCAGCGTGAAGTGGGGATCGATACCGA
GTCCTTCGAGGTGGCGCTGCGCAATACGCTGCGTCAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCTCGCGTGAGA
CCATGGAGCACGCGCTGACCTTCGCCGAGACCGGTCACCTGTGTCTTGCGACGTTGCATGCCAACAATGCCAACCAGGCG
CTGGACCGCATCATCCACTTCTTCCCGGAGGAGCGTCATGAACAGGTGTGGATGGATCTGTCGCTGAACCTCAAGGGCAT
CGTGGCCCAGCAGCTGTTGCCTCACAAGAGTGGCAACACCACCCAGCGCGTGCCGGCCATTGAGGTGATGCTGCGTTCGC
CGCTGATCGTCGACCTGATCCGCAAGGGCGCGGTGGTCGAGATCAAGGACGTCATGAAGCGCTCGCAGCAGCAGGGCATG
ATGACCTTCGATCAATCGCTGTACGCCTTGCATCAGCAGGGCTTGATCACCGAGGAAGTGGCATTGGCGCATGCCGATTC
CGCCAATGACCTGCGCCTGATGATCAAGTTCGGTGATTCCAACAGTGCCCAGGAAGCCCAGCTGGATGTCCTGAATGCCG
CCAGTCGCTTCTCGCTGCAAGGGGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (84-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64.525	93.229	0.602
pilU	Vibrio cholerae strain A1552	56.64	96.094	0.544
pilU	Acinetobacter baylyi ADP1	55.932	92.188	0.516
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.348	89.844	0.398
pilT	Neisseria meningitidis 8013	40.816	89.323	0.365
pilT	Pseudomonas aeruginosa PAK	41.742	86.719	0.362