Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV04_RS12495 Genome accession   NZ_AP024893
Coordinates   2715360..2717048 (+) Length   562 a.a.
NCBI ID   WP_171138259.1    Uniprot ID   -
Organism   Vibrio plantisponsor strain CECT 7581     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2710360..2722048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV04_RS12465 dsbC 2710692..2711447 (-) 756 WP_171138256.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  OCV04_RS12470 xerD 2711459..2712367 (-) 909 WP_102940970.1 site-specific tyrosine recombinase XerD -
  OCV04_RS12475 fldB 2712548..2713066 (+) 519 WP_102940969.1 flavodoxin FldB -
  OCV04_RS12480 ampD 2713069..2713611 (-) 543 WP_171138257.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV04_RS12485 nadC 2713745..2714632 (+) 888 WP_042484530.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV04_RS12490 - 2714901..2715344 (+) 444 WP_171138258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCV04_RS12495 pilB 2715360..2717048 (+) 1689 WP_171138259.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV04_RS20780 - 2717184..2717360 (-) 177 WP_390632044.1 hypothetical protein -
  OCV04_RS12500 pilC 2717304..2718293 (+) 990 WP_390632046.1 type II secretion system F family protein Machinery gene
  OCV04_RS12505 pilD 2718373..2719242 (+) 870 WP_171138260.1 A24 family peptidase Machinery gene
  OCV04_RS12510 coaE 2719246..2719854 (+) 609 WP_102940963.1 dephospho-CoA kinase -
  OCV04_RS12515 zapD 2719886..2720626 (+) 741 WP_102940962.1 cell division protein ZapD -
  OCV04_RS12520 yacG 2720672..2720875 (+) 204 WP_171138261.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62155.94 Da        Isoelectric Point: 5.5041

>NTDB_id=89222 OCV04_RS12495 WP_171138259.1 2715360..2717048(+) (pilB) [Vibrio plantisponsor strain CECT 7581]
MITNLITLLRQANLLSLEQEQSVVSHIEASGTDVPEALIQLGFIHSSELANYLQQQLAMPVTQLNEFDYAQVCQQLGLRD
LITRFNALPIQNQNNQLLLAVSDPTHPSVEDEFRFTTGLNVELALADHKSLQAAIRRLYGKTINDEAKQNKEISQDELAS
LVQLSDDELQSIEDLSKDESPVSRFINQVLLDAVRKGASDIHFEPYEDVYRIRLRCDGILVETHRPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNRDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLNIDKLGYDEVQKQHYLDALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTSEINISTAEDPVEINLSGINQVQVQPKIGFGFSDALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCSHCKIATTPSVTLQAQFNLSADE
TIYQANHLGCTECTGGYSGRVGIYEVMAFNDVLAEAVMNKSSVAQIETIAKANGMSTLLDSGVQKLKQGVTSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=89222 OCV04_RS12495 WP_171138259.1 2715360..2717048(+) (pilB) [Vibrio plantisponsor strain CECT 7581]
ATGATAACCAACCTAATCACCCTACTGCGCCAAGCTAATTTATTAAGCTTGGAGCAAGAGCAGAGTGTGGTGAGCCATAT
TGAAGCTTCTGGCACTGATGTGCCGGAAGCTTTAATTCAGCTTGGGTTTATTCACTCGTCTGAACTCGCTAACTATCTAC
AGCAACAACTAGCGATGCCAGTCACTCAACTCAATGAGTTTGATTATGCACAGGTATGTCAACAGCTTGGTTTAAGAGAT
CTCATCACTCGCTTTAATGCTCTGCCAATTCAAAACCAAAATAACCAACTGCTTCTGGCGGTATCAGATCCAACACACCC
GAGTGTAGAAGATGAGTTTCGCTTCACAACTGGCTTAAACGTTGAACTGGCACTCGCCGATCATAAATCATTGCAAGCCG
CCATTCGTCGCCTCTATGGCAAAACAATCAACGACGAAGCTAAGCAGAACAAAGAGATCAGCCAAGATGAACTTGCAAGC
TTAGTTCAACTTTCTGATGATGAGCTGCAATCGATTGAGGATCTCAGTAAAGACGAATCGCCGGTAAGCCGATTTATCAA
TCAAGTGTTACTTGATGCTGTGCGCAAAGGAGCTTCCGATATTCACTTTGAACCCTATGAAGATGTGTATCGTATTCGCC
TACGTTGTGATGGTATTTTAGTCGAAACTCACCGCCCTGCGAGCCATCTCAGTCGGCGGCTTGCTGCGCGTATTAAAATA
CTTTCAAAGCTGGATATTGCCGAACGCCGACTTCCCCAAGACGGACGAATTAAACTTCGCTTAAATCGCGATACTGCGAT
TGATATGCGAGTTTCATCGCTGCCAACTTTATGGGGAGAGAAGATTGTATTACGTCTGCTTGATAGCAGCGCAGCCAATC
TCAACATCGACAAACTCGGCTATGACGAAGTGCAAAAGCAGCACTATTTAGATGCCCTTAAAAAGCCGCAAGGCATGATC
TTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCTCTTTATACAGGGCTACGCATACTCAATACCTCTGAAATTAA
TATCTCCACCGCGGAAGATCCGGTGGAAATCAACCTCTCGGGCATTAATCAGGTGCAGGTACAACCTAAGATAGGTTTTG
GCTTCTCTGATGCACTACGTTCCTTTCTTCGTCAGGACCCTGATGTGGTGATGGTGGGTGAGATTCGAGACCTCGAAACT
GCGGAAATAGCGGTGAAAGCAGCTCAAACTGGACACTTGGTGTTATCAACACTCCATACCAACTCTGCCGCCGAAACCGT
CATACGTTTAGCCAACATGGGTGTTGAACCCTTCAACCTCGCATCGTCTCTGAGTTTAATCATCGCCCAACGTCTGGCTC
GCCGTTTATGCAGCCACTGCAAAATAGCCACTACGCCATCCGTCACCTTGCAAGCTCAGTTTAACTTGTCAGCGGACGAA
ACCATATACCAAGCCAACCACTTAGGCTGTACCGAGTGCACCGGAGGATATTCTGGACGTGTCGGAATCTACGAAGTGAT
GGCTTTCAATGACGTTCTTGCTGAAGCCGTCATGAATAAGTCAAGTGTGGCGCAAATCGAAACCATCGCTAAGGCCAATG
GCATGAGCACTTTGCTGGATTCTGGCGTACAGAAGTTAAAGCAAGGTGTCACCAGTTTTGCCGAGTTACAACGTGTTTTG
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

79.359

100

0.794
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.701
  pilB Vibrio campbellii strain DS40M4

69.929

100

0.699
  pilB Acinetobacter baumannii D1279779

48.629

97.331

0.473
  pilB Legionella pneumophila strain ERS1305867

49.245

94.306

0.464
  pilB Acinetobacter baylyi ADP1

48.757

93.06

0.454
  pilF Neisseria gonorrhoeae MS11

43.363

100

0.436
  pilF Thermus thermophilus HB27

38.49

96.619

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

39.155

92.705

0.363

Multiple sequence alignment