Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV27_RS12645 Genome accession   NZ_AP024889
Coordinates   2775732..2777396 (+) Length   554 a.a.
NCBI ID   WP_125319698.1    Uniprot ID   A0A3R9F900
Organism   Vibrio pectenicida strain LMG 19642     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2770732..2782396
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV27_RS12615 - 2771059..2771811 (-) 753 WP_125319692.1 thioredoxin fold domain-containing protein -
  OCV27_RS12620 xerD 2771849..2772703 (-) 855 WP_245986645.1 site-specific tyrosine recombinase XerD -
  OCV27_RS12625 fldB 2772909..2773430 (+) 522 WP_125319694.1 flavodoxin FldB -
  OCV27_RS12630 ampD 2773525..2774070 (-) 546 WP_125319695.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV27_RS12635 nadC 2774203..2775090 (+) 888 WP_125319696.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV27_RS12640 - 2775304..2775735 (+) 432 WP_125319697.1 pilin -
  OCV27_RS12645 pilB 2775732..2777396 (+) 1665 WP_125319698.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  OCV27_RS12650 - 2777410..2778627 (+) 1218 WP_125319699.1 type II secretion system F family protein -
  OCV27_RS12655 pilD 2778677..2779546 (+) 870 WP_125319700.1 prepilin peptidase Machinery gene
  OCV27_RS12660 coaE 2779548..2780162 (+) 615 WP_125319701.1 dephospho-CoA kinase -
  OCV27_RS12665 zapD 2780190..2780930 (+) 741 WP_125319702.1 cell division protein ZapD -
  OCV27_RS12670 yacG 2780964..2781158 (+) 195 WP_125319703.1 DNA gyrase inhibitor YacG -
  OCV27_RS12675 rplS 2781246..2781599 (-) 354 WP_045977783.1 50S ribosomal protein L19 -
  OCV27_RS12680 trmD 2781643..2782383 (-) 741 WP_125319704.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 554 a.a.        Molecular weight: 61646.76 Da        Isoelectric Point: 6.8344

>NTDB_id=89183 OCV27_RS12645 WP_125319698.1 2775732..2777396(+) (pilB) [Vibrio pectenicida strain LMG 19642]
MSQLPQLLCNANFINAQQVEATLATMKAEQIDACSALTRLDNVTPITLAETLSKLFNLPVVSLDSFDYQSCCEQLGVSHL
IHQHLALPLEITHCQLLIAVADPSNSITEQEFNFMTGLNIKCVLVEVSQLTHAIHSLYGSESANQQYNTKHISSNELEHL
VDDVRSKEEGIEQDNSLISRYIHQIILEAVRKKVSDIHFEPYDGWFRIRFRCDGLLIEAHQPPHQIYQRLVSRLKILAQL
DISERRQPQDGRITFNLTESNIIHIRISTLPTMWGEKIVLRLQNNQVTNLDLTFLGYDSYQRQVFTQALHKPQGLILITG
PTGSGKTLSLYSGLTLLNKGSINISTAEDPVEISLPGINQVQIQPQIGFGFAQALRAFLRQDPDVIMVGEIRDKETADIA
TKAAQTGHLVLATLHTNSSTEAIVRLKNIGIESYNIAASLSLVIAQRLVRRLCPLCKCADTVPANIPQVKGTVYYQASAN
GCNACNQGYLGRIGIYEMLSITPELTQSILKQDTQAQLDKLAKKQGMKTLNQLGIQKVCEGVTSHAELQRVLVR

Nucleotide


Download         Length: 1665 bp        

>NTDB_id=89183 OCV27_RS12645 WP_125319698.1 2775732..2777396(+) (pilB) [Vibrio pectenicida strain LMG 19642]
ATGAGCCAGCTTCCTCAATTACTGTGCAATGCAAATTTTATTAATGCTCAGCAAGTTGAAGCTACTCTTGCGACAATGAA
AGCAGAACAAATTGACGCTTGCTCTGCTCTAACTCGACTTGATAATGTTACGCCTATAACACTAGCTGAAACTCTTAGTA
AATTATTTAATCTCCCCGTGGTCTCACTGGACAGTTTTGACTATCAATCATGTTGTGAGCAACTAGGGGTTAGCCATTTA
ATCCACCAGCACTTGGCTTTACCGTTGGAAATTACACACTGTCAATTGCTGATTGCGGTTGCAGACCCCTCAAACTCAAT
TACAGAGCAAGAGTTTAACTTCATGACAGGCTTAAATATAAAATGCGTGCTCGTCGAAGTTAGTCAATTAACCCATGCTA
TACACTCACTGTATGGCTCAGAGAGTGCTAATCAACAATACAATACAAAACATATAAGCAGTAACGAACTAGAGCATTTA
GTCGATGATGTTAGATCTAAAGAAGAAGGTATTGAGCAAGATAATTCTCTTATTAGCCGTTATATACATCAAATAATATT
GGAAGCCGTACGTAAGAAAGTTTCAGACATTCACTTTGAACCCTACGATGGCTGGTTTAGAATCCGATTTCGCTGCGACG
GGTTATTGATAGAAGCACACCAACCTCCTCATCAAATTTATCAAAGGTTAGTATCTAGGCTCAAAATCTTAGCGCAGCTC
GATATATCTGAGCGCCGACAACCTCAAGACGGCCGTATCACTTTCAACTTAACAGAGTCGAATATTATCCACATCCGCAT
TTCAACTTTGCCCACTATGTGGGGCGAGAAGATAGTATTAAGACTACAAAACAATCAAGTAACAAACCTAGATCTTACCT
TTCTGGGGTACGACTCATATCAACGTCAAGTGTTTACCCAAGCACTCCACAAGCCTCAAGGTCTGATACTTATCACAGGC
CCAACCGGAAGTGGTAAAACGCTATCTTTATACAGTGGGTTAACTTTACTAAACAAAGGGAGTATCAATATTTCAACAGC
AGAAGATCCTGTGGAAATCAGCTTACCTGGTATCAACCAAGTCCAGATTCAGCCTCAAATTGGATTTGGGTTTGCTCAAG
CACTGCGAGCCTTTCTCCGTCAAGATCCAGATGTAATTATGGTGGGAGAAATACGTGATAAAGAAACCGCAGACATTGCT
ACAAAGGCTGCTCAAACCGGTCATCTTGTTCTGGCAACTCTACACACTAACTCAAGCACTGAAGCTATTGTACGCTTAAA
AAATATAGGCATTGAATCTTATAACATCGCGGCTTCACTCAGCCTTGTCATTGCCCAAAGATTAGTCCGTCGACTTTGCC
CTCTCTGTAAATGTGCAGACACAGTGCCTGCAAATATCCCCCAAGTAAAAGGGACCGTTTATTATCAAGCATCGGCTAAT
GGATGCAATGCTTGTAATCAGGGATATCTTGGGAGAATAGGTATCTACGAAATGTTAAGTATTACCCCAGAGTTAACCCA
ATCGATACTCAAGCAAGATACTCAAGCACAGCTTGATAAACTTGCTAAAAAACAAGGGATGAAAACACTTAACCAGTTAG
GTATACAAAAAGTTTGTGAAGGTGTCACCAGTCACGCAGAACTGCAACGAGTATTGGTACGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9F900

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

52.065

100

0.523
  pilB Vibrio cholerae strain A1552

51.429

100

0.52
  pilB Vibrio campbellii strain DS40M4

51.434

100

0.518
  pilB Acinetobacter baumannii D1279779

43.214

100

0.437
  pilB Acinetobacter baylyi ADP1

42.162

100

0.422
  pilB Legionella pneumophila strain ERS1305867

43.487

94.224

0.41
  pilB Haemophilus influenzae Rd KW20

44.77

86.282

0.386
  pilB Haemophilus influenzae 86-028NP

44.351

86.282

0.383
  pilF Neisseria gonorrhoeae MS11

40.039

93.321

0.374

Multiple sequence alignment