Detailed information NTG0089184

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCV27_RS12655
• Genome accession: NZ_AP024889
• Coordinates: 2778677..2779546 (+)
• Length: 289 a.a.
• NCBI ID: WP_125319700.1
• Uniprot ID: A0A427U7H2
• Organism: Vibrio pectenicida strain LMG 19642
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2773677..2784546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCV27_RS12635	nadC	2774203..2775090 (+)	888	WP_125319696.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OCV27_RS12640	-	2775304..2775735 (+)	432	WP_125319697.1	pilin	-
OCV27_RS12645	pilB	2775732..2777396 (+)	1665	WP_125319698.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
OCV27_RS12650	-	2777410..2778627 (+)	1218	WP_125319699.1	type II secretion system F family protein	-
OCV27_RS12655	pilD	2778677..2779546 (+)	870	WP_125319700.1	prepilin peptidase	Machinery gene
OCV27_RS12660	coaE	2779548..2780162 (+)	615	WP_125319701.1	dephospho-CoA kinase	-
OCV27_RS12665	zapD	2780190..2780930 (+)	741	WP_125319702.1	cell division protein ZapD	-
OCV27_RS12670	yacG	2780964..2781158 (+)	195	WP_125319703.1	DNA gyrase inhibitor YacG	-
OCV27_RS12675	rplS	2781246..2781599 (-)	354	WP_045977783.1	50S ribosomal protein L19	-
OCV27_RS12680	trmD	2781643..2782383 (-)	741	WP_125319704.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
OCV27_RS12685	rimM	2782429..2782977 (-)	549	WP_125319705.1	ribosome maturation factor RimM	-
OCV27_RS12690	rpsP	2783000..2783248 (-)	249	WP_125319706.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32649.83 Da
• Isoelectric Point: 7.8198

>NTDB_id=89184 OCV27_RS12655 WP_125319700.1 2778677..2779546(+) (pilD) [Vibrio pectenicida strain LMG 19642]
MNLFFYYPWLFPTLATIFGLIIGSFLNVVIHRLPKIMEQEWHKECAECFPEYQISPPVENFSLSKPRSQCPSCKTKIRNI
DNIPLISWLMLRGKCNHCQAKISVRYPLVELLSALLSFTVAYQFEFSYFAVALLCFTFVLIAATFIDLDTMLLPDQLTLP
LVWSGIYISLIGVSPVSLQDAVIGAISGYLCLWTVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQYLPIIVLLSSLVGL
VFGIIQLRLKKQGIDKAFPFGPYLAIAGWISALWGEQILSWYFTQVIGV

Nucleotide

• Length: 870 bp

>NTDB_id=89184 OCV27_RS12655 WP_125319700.1 2778677..2779546(+) (pilD) [Vibrio pectenicida strain LMG 19642]
ATGAATCTCTTTTTTTATTACCCGTGGTTATTTCCAACCCTTGCAACCATATTTGGTCTGATCATCGGAAGCTTCCTCAA
TGTCGTCATTCATCGACTACCTAAGATTATGGAACAAGAATGGCATAAAGAATGCGCGGAATGTTTCCCTGAGTATCAAA
TATCACCTCCAGTAGAAAACTTTAGCCTTAGCAAGCCACGTTCTCAATGCCCAAGTTGCAAAACTAAAATTAGAAACATT
GATAATATCCCTCTCATTAGCTGGCTGATGTTAAGAGGTAAATGTAATCATTGCCAAGCCAAAATCAGCGTACGATACCC
ACTTGTCGAGCTGCTATCTGCCCTATTAAGCTTTACCGTCGCATATCAATTTGAGTTCAGCTACTTTGCTGTTGCATTAC
TGTGCTTTACTTTTGTCCTAATCGCTGCAACTTTTATCGATTTAGACACTATGCTCTTACCGGACCAACTAACTCTTCCG
CTTGTATGGTCAGGTATATATATTTCTCTAATTGGGGTAAGCCCAGTTTCTTTGCAAGATGCTGTTATAGGTGCCATTAG
TGGGTACCTATGTTTGTGGACTGTCTACTGGGGGTTCAAACTTTTGACAGGCAAAGAAGGAATGGGCTACGGTGATTTTA
AGTTATTAGCAGCTTTAGGGGCTTGGCTTGGTTGGCAATATTTGCCCATTATCGTCTTACTTTCTTCTTTAGTTGGGTTA
GTTTTTGGAATCATTCAACTACGCTTGAAAAAACAAGGAATTGATAAAGCATTTCCTTTTGGACCGTACCTTGCTATTGC
GGGATGGATAAGCGCACTCTGGGGAGAACAGATTTTAAGCTGGTACTTCACTCAAGTTATTGGAGTCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A427U7H2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	73.702	100	0.737
pilD	Vibrio cholerae strain A1552	71.181	99.654	0.709
pilD	Acinetobacter nosocomialis M2	53.731	92.734	0.498
pilD	Acinetobacter baumannii D1279779	53.358	92.734	0.495
pilD	Neisseria gonorrhoeae MS11	47.292	95.848	0.453