Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCU77_RS02105 Genome accession   NZ_AP024852
Coordinates   437027..438712 (-) Length   561 a.a.
NCBI ID   WP_107302991.1    Uniprot ID   A0A2T3P2A5
Organism   Photobacterium swingsii strain CECT 7576     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432027..443712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU77_RS02075 mutT 432690..433112 (+) 423 WP_048899242.1 8-oxo-dGTP diphosphatase MutT -
  OCU77_RS02080 yacG 433171..433377 (-) 207 WP_084711799.1 DNA gyrase inhibitor YacG -
  OCU77_RS02085 zapD 433386..434123 (-) 738 WP_048899283.1 cell division protein ZapD -
  OCU77_RS02090 coaE 434164..434769 (-) 606 WP_048899243.1 dephospho-CoA kinase -
  OCU77_RS02095 pilD 434772..435650 (-) 879 WP_048899244.1 A24 family peptidase Machinery gene
  OCU77_RS02100 pilC 435770..436990 (-) 1221 WP_048899245.1 type II secretion system F family protein Machinery gene
  OCU77_RS02105 pilB 437027..438712 (-) 1686 WP_107302991.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCU77_RS02110 - 438712..439161 (-) 450 WP_048899246.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCU77_RS02115 nadC 439469..440368 (-) 900 WP_048899284.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCU77_RS02120 ampD 440566..441132 (+) 567 WP_048899247.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCU77_RS02125 pdhR 441418..442191 (+) 774 WP_048899248.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62211.78 Da        Isoelectric Point: 5.9006

>NTDB_id=88797 OCU77_RS02105 WP_107302991.1 437027..438712(-) (pilB) [Photobacterium swingsii strain CECT 7576]
MQTTLTSVLHQAGVLNLMQQQEVSSFAHAEGLSIPSALADLNILTSDDLARQLESILSIALTDIHQYNYQGCCESLRQRE
SILRHRVIPLKLTETTLFLGTSDPTNVAAQDEFRFATGKQVEPLLLSNKQLESAIRRLYGSDVGETSHQRGISEDDLDQL
VDLSEGEIEQENDLSQDTAPVTRYINQVLLDAVRKKASDIHFEPYEKFFRIRFRCDGLLHQYSSPPAHLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSPTVTVDLRVSTLPTLWGEKVVLRILDSTSASLNIDILGYNQQQKAAYLAALQQPQGMIL
MTGPTGSGKTVSLYTGLKILNTDERNISTAEDPVEINLPGINQVHINNQAGLGFADALRSFLRQDPDVVMVGEIRDIETA
AIAVKAAQTGHLVLSTLHTNSAAETLTRLTNMGVEDFNLASSLSLIIAQRLARRLCNACKQPHPLSNMHRERLAIPTDAE
LYQANEQGCDDCNKGYLGRVGIYEVMTFSRELAEALMAKATTLQLEEIACQQGMQTLNQAGIEKLCQGTTSLTELQRVLH
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=88797 OCU77_RS02105 WP_107302991.1 437027..438712(-) (pilB) [Photobacterium swingsii strain CECT 7576]
ATGCAAACTACCCTCACTTCAGTACTGCATCAAGCGGGTGTACTTAACCTGATGCAGCAGCAAGAAGTCAGTAGTTTCGC
CCACGCCGAGGGGCTCTCGATCCCCTCGGCGTTGGCCGATCTTAATATACTGACCAGTGACGATTTAGCCCGCCAGCTCG
AATCTATTCTTAGTATCGCCTTGACCGATATTCATCAATACAATTACCAAGGCTGTTGCGAGAGCTTACGTCAGCGCGAG
AGCATTTTGCGCCATCGTGTGATTCCGCTTAAGCTCACCGAGACAACTTTGTTTTTGGGCACCAGCGATCCAACCAACGT
TGCCGCTCAAGATGAATTCCGCTTTGCCACCGGCAAACAAGTCGAGCCGCTACTACTCAGTAATAAGCAACTAGAAAGTG
CGATCCGTCGGCTTTACGGCAGTGATGTCGGTGAAACCTCCCACCAGCGTGGGATCAGCGAAGATGACTTGGATCAGTTG
GTCGATCTGAGCGAAGGCGAGATTGAACAAGAGAATGATCTAAGCCAAGACACCGCACCTGTTACCCGTTATATCAACCA
AGTACTACTCGATGCGGTACGAAAAAAAGCCTCTGATATTCACTTTGAACCGTACGAAAAGTTCTTTCGAATTCGTTTTC
GTTGTGATGGTTTGCTACACCAATATTCCAGTCCACCAGCACATTTATCTCGGCGGTTATCCACTCGCTTAAAGGTGATG
TCGAAGCTCAATATTGCCGAGCGTCGCCAACCACAAGATGGACGAATAAAACTCAAACTTAGCCCAACCGTTACCGTTGA
TTTACGGGTATCTACCCTCCCGACTCTGTGGGGCGAAAAGGTAGTATTACGGATATTAGACAGCACCAGCGCCAGCTTAA
ACATTGATATTCTAGGTTATAACCAACAACAAAAAGCAGCTTATCTTGCTGCATTGCAACAACCGCAAGGGATGATTTTA
ATGACAGGGCCAACGGGTAGCGGCAAAACCGTCTCGCTTTATACTGGCCTTAAGATCCTCAATACCGATGAGCGTAATAT
TTCCACCGCTGAAGATCCGGTCGAAATAAACTTACCCGGTATTAATCAGGTTCATATCAACAACCAAGCTGGGCTAGGCT
TTGCCGATGCACTGCGATCATTTTTACGCCAAGATCCCGATGTGGTCATGGTCGGTGAAATTCGCGATATAGAAACCGCC
GCCATTGCGGTCAAAGCCGCTCAAACCGGGCACTTGGTACTATCAACCCTACACACGAATTCCGCCGCCGAAACCCTGAC
CCGTTTGACCAATATGGGGGTAGAAGATTTTAACTTAGCCTCATCGCTTAGCTTGATCATCGCTCAACGACTGGCGCGGC
GTTTATGTAACGCTTGTAAACAGCCCCACCCTTTATCAAATATGCATAGGGAGCGACTTGCCATCCCTACCGATGCAGAG
CTTTATCAAGCCAATGAACAAGGCTGTGATGATTGCAACAAAGGCTATCTGGGTCGAGTCGGTATTTATGAAGTCATGAC
CTTTAGCCGTGAACTGGCGGAAGCCTTAATGGCCAAAGCAACGACGTTGCAACTAGAGGAAATTGCCTGTCAGCAAGGAA
TGCAAACCCTCAATCAAGCAGGAATAGAAAAGCTGTGCCAAGGCACCACCAGCTTAACTGAATTGCAGCGCGTCCTGCAT
TTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3P2A5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

60.498

100

0.606
  pilB Vibrio campbellii strain DS40M4

60.32

100

0.604
  pilB Vibrio parahaemolyticus RIMD 2210633

60.32

100

0.604
  pilB Legionella pneumophila strain ERS1305867

48.282

98.574

0.476
  pilB Acinetobacter baylyi ADP1

54.357

85.918

0.467
  pilB Acinetobacter baumannii D1279779

50.677

92.157

0.467
  pilF Neisseria gonorrhoeae MS11

44.227

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

39.265

92.157

0.362

Multiple sequence alignment