Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RS899_RS24120 Genome accession   NZ_CP135919
Coordinates   5257285..5259357 (-) Length   690 a.a.
NCBI ID   WP_233297421.1    Uniprot ID   -
Organism   Pseudomonas sp. C9-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5252285..5264357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS899_RS24105 - 5253906..5255594 (-) 1689 WP_233297419.1 AAA family ATPase -
  RS899_RS24110 aroB 5255601..5256707 (-) 1107 WP_315808248.1 3-dehydroquinate synthase -
  RS899_RS24115 aroK 5256758..5257249 (-) 492 WP_052239087.1 shikimate kinase AroK -
  RS899_RS24120 pilQ 5257285..5259357 (-) 2073 WP_233297421.1 type IV pilus secretin PilQ Machinery gene
  RS899_RS24125 pilP 5259411..5259935 (-) 525 WP_024764145.1 type 4a pilus biogenesis lipoprotein PilP -
  RS899_RS24130 pilO 5259932..5260585 (-) 654 WP_233297423.1 type 4a pilus biogenesis protein PilO -
  RS899_RS24135 pilN 5260582..5261175 (-) 594 WP_315808249.1 type 4a pilus biogenesis protein PilN -
  RS899_RS24140 comM 5261175..5262239 (-) 1065 WP_024764148.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 690 a.a.        Molecular weight: 74009.95 Da        Isoelectric Point: 5.0922

>NTDB_id=887678 RS899_RS24120 WP_233297421.1 5257285..5259357(-) (pilQ) [Pseudomonas sp. C9-3]
MNKTLSRLGISVLAAVFAPTLLAANLQSVDVAALPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIVNLATLVPYSTRAEGSNLYVVVGAGAAPSSSYASAAPTAAAAKPAQPKPYIPAGKAIRN
IDFQRGEQGEGNVVIDLSDPTVSPDIQEQGGKIRLDFPRTQLPDNLRVRLDVKDFATPVQLVNASAVGDKASITIEPTGL
YDYLVYQTDNRMTVSIKPLTQDDAEKRKKDAFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFSSVTGGAAA
PGVAAAAQGENRGSITVDDRTNSIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYTKELGVNWSGSLSGHGN
FDVSGGTNSTGNTFVDLGTSNVTPTSGISIGFTTNNTILDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKGSEIPYQE
ASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVNKDEPDYANALNGVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQ
TKSTAKVPFLGDVPMIGRLFKNDYVTDSKNELLVFLTPRIMNNQAIAVGR

Nucleotide


Download         Length: 2073 bp        

>NTDB_id=887678 RS899_RS24120 WP_233297421.1 5257285..5259357(-) (pilQ) [Pseudomonas sp. C9-3]
ATGAATAAAACGCTTTCCCGCCTGGGCATTTCCGTGCTCGCGGCCGTATTCGCACCGACCCTGCTGGCTGCAAACCTGCA
GAGTGTCGACGTGGCGGCCCTGCCGGGCGACCGCGTGGAGCTCAAGCTCGGCTTCGACGAGCCGGTGGCTGCACCGCGTG
GCTATACCATCGAGCAGCCCGCGCGTATCGCACTGGATCTGCCGGGCGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAACGCCCGCAGCCTGACCGTGGTCGAGGCCAAGGACCGTACGCGCCTCATCGTCAACCTGGCTACCCT
GGTGCCTTACTCCACTCGTGCGGAAGGTTCGAATCTTTACGTTGTGGTGGGTGCTGGCGCTGCGCCGTCGAGTTCCTATG
CTTCTGCTGCGCCAACTGCGGCCGCCGCCAAGCCCGCCCAGCCCAAGCCTTACATTCCGGCAGGCAAGGCGATCCGCAAC
ATCGATTTCCAGCGTGGCGAACAGGGCGAAGGCAATGTCGTGATCGATCTGTCCGATCCGACCGTCAGCCCCGACATTCA
GGAGCAGGGTGGCAAGATTCGTCTGGACTTCCCGCGCACTCAGCTGCCGGACAACCTGCGCGTCCGCCTGGATGTGAAAG
ACTTTGCCACGCCGGTACAGCTCGTCAACGCTTCCGCTGTCGGCGACAAGGCCAGCATCACCATCGAGCCGACCGGGCTG
TACGACTACCTCGTCTACCAGACCGACAATCGCATGACCGTCAGCATCAAGCCGCTGACCCAGGACGATGCCGAGAAGCG
CAAGAAGGACGCCTTCGCCTACACCGGTGAAAAGCTCTCGCTGAACTTCCAGGACATCGATGTGCGCTCGGTGCTGCAAC
TGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGACACCGTGCAGGGCAACATCACCCTGCGCCTGCAGAACGTG
CCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTGGGCAACGTGCTGCTGGTTGC
GCCGGCTGATGAAATCGCCGCTCGCGAGCGTCAGGAACTGGAGGCGCAGAAGCAGATTGCCGAGCTCGCCCCCCTGCGTC
GTGAGCTGATCCAGGTGAACTACGCCAAGGCGGCGGATATCGCCAAGCTGTTCAGCTCCGTCACTGGCGGCGCTGCTGCC
CCGGGTGTCGCTGCCGCGGCTCAGGGCGAAAATCGCGGCTCGATCACCGTCGACGACCGTACCAACAGCATCATCGCCTA
CCAGACTCAGGATCGCCTGGACGAGCTGCGCCGCATCGTCGCACAGCTGGACATCCCGGTTCGCCAGGTGATGATCGAGG
CGCGGATTGTCGAGGCCAACGTCGATTACACCAAGGAGCTGGGCGTGAACTGGAGCGGTTCGTTGTCCGGTCACGGCAAC
TTCGATGTTTCGGGCGGCACCAACAGCACAGGCAATACCTTCGTCGATCTGGGAACCTCGAATGTCACGCCGACCTCGGG
TATCAGCATCGGCTTCACCACCAACAACACCATCCTGGATCTCCAGTTGAACGCCATGGAGAAAACCGGCAACGGTGAAG
TGGTCTCGCAGCCGAAGGTTGTCACCTCTGACAAGGAAACGGCGAAGATCCTCAAGGGCTCGGAGATTCCTTACCAGGAA
GCCAGCTCCAGCGGCGCGACCTCGGTGTCCTTCAAGGAGGCGGCGCTGTCCCTGGAAGTGACCCCGCAGATCACGCCGGA
CAACCGCATCATCATGGAAGTGAAGGTCAACAAGGACGAGCCGGACTACGCCAACGCCCTGAACGGCGTACCGCCGATCA
AGAAGAACGAAGTGAACGCGAAGGTGCTGGTCAACGATGGCGAAACCATCGTGATCGGTGGCGTGTTCTCCAACACCCAG
ACCAAATCGACGGCTAAGGTGCCGTTCCTGGGCGACGTGCCGATGATCGGCCGCCTGTTCAAGAACGACTACGTGACCGA
CAGCAAGAACGAGCTGCTGGTTTTCCTTACCCCGCGGATCATGAATAATCAGGCCATTGCAGTCGGCCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-449)

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(40-121)

Bacterial type II and III secretion system protein (Secretin)

(525-681)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.978

100

0.816
  comQ Acinetobacter baylyi ADP1

42.63

100

0.428
  pilQ Acinetobacter baumannii strain A118

39.51

100

0.42
  pilQ Acinetobacter baumannii D1279779

39.51

100

0.42
  pilQ Legionella pneumophila strain Lp02

39.086

98.261

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.086

98.261

0.384