Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RQR13_RS05040 Genome accession   NZ_CP135194
Coordinates   1024477..1024911 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 102426     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019477..1029911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQR13_RS05010 tadA 1019587..1020087 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  RQR13_RS05015 - 1020102..1020953 (-) 852 WP_014391366.1 thymidylate synthase -
  RQR13_RS05020 lgt 1020950..1021762 (-) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  RQR13_RS05025 - 1021772..1022566 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RQR13_RS05030 rppH 1022569..1023165 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RQR13_RS05035 ampD 1023751..1024317 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RQR13_RS05040 pilA 1024477..1024911 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  RQR13_RS05045 pilB 1024911..1026296 (+) 1386 WP_014391364.1 GspE/PulE family protein Machinery gene
  RQR13_RS05050 - 1026296..1027507 (+) 1212 WP_315505790.1 type II secretion system F family protein -
  RQR13_RS05055 - 1027507..1028229 (+) 723 WP_041423102.1 A24 family peptidase -
  RQR13_RS05060 coaE 1028244..1028864 (+) 621 WP_014391361.1 dephospho-CoA kinase -
  RQR13_RS05065 yacG 1028875..1029078 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RQR13_RS05070 - 1029078..1029347 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=886140 RQR13_RS05040 WP_005741096.1 1024477..1024911(+) (pilA) [Pasteurella multocida strain 102426]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=886140 RQR13_RS05040 WP_005741096.1 1024477..1024911(+) (pilA) [Pasteurella multocida strain 102426]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTTACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368