Detailed information NTG0886141

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: RQR13_RS05045
• Genome accession: NZ_CP135194
• Coordinates: 1024911..1026296 (+)
• Length: 461 a.a.
• NCBI ID: WP_014391364.1
• Uniprot ID: -
• Organism: Pasteurella multocida strain 102426
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1019911..1031296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RQR13_RS05015	-	1020102..1020953 (-)	852	WP_014391366.1	thymidylate synthase	-
RQR13_RS05020	lgt	1020950..1021762 (-)	813	WP_014391365.1	prolipoprotein diacylglyceryl transferase	-
RQR13_RS05025	-	1021772..1022566 (-)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
RQR13_RS05030	rppH	1022569..1023165 (-)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RQR13_RS05035	ampD	1023751..1024317 (-)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RQR13_RS05040	pilA	1024477..1024911 (+)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RQR13_RS05045	pilB	1024911..1026296 (+)	1386	WP_014391364.1	GspE/PulE family protein	Machinery gene
RQR13_RS05050	-	1026296..1027507 (+)	1212	WP_315505790.1	type II secretion system F family protein	-
RQR13_RS05055	-	1027507..1028229 (+)	723	WP_041423102.1	A24 family peptidase	-
RQR13_RS05060	coaE	1028244..1028864 (+)	621	WP_014391361.1	dephospho-CoA kinase	-
RQR13_RS05065	yacG	1028875..1029078 (+)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
RQR13_RS05070	-	1029078..1029347 (+)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RQR13_RS05075	-	1029796..1031169 (+)	1374	WP_005722571.1	sodium:alanine symporter family protein	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 52255.91 Da
• Isoelectric Point: 6.5608

>NTDB_id=886141 RQR13_RS05045 WP_014391364.1 1024911..1026296(+) (pilB) [Pasteurella multocida strain 102426]
MFHENPLCTVSTMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPHFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFTELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide

• Length: 1386 bp

>NTDB_id=886141 RQR13_RS05045 WP_014391364.1 1024911..1026296(+) (pilB) [Pasteurella multocida strain 102426]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTACAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAATATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCACTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTACTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGAAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (128-394)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (19-93)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Haemophilus influenzae 86-028NP	67.77	98.265	0.666
pilB	Haemophilus influenzae Rd KW20	67.329	98.265	0.662
pilB	Glaesserella parasuis strain SC1401	58.59	98.482	0.577
pilB	Acinetobacter baumannii D1279779	39.757	100	0.425
pilB	Acinetobacter baylyi ADP1	37.876	100	0.41
pilB	Vibrio parahaemolyticus RIMD 2210633	39.293	100	0.41
pilB	Vibrio campbellii strain DS40M4	39.04	100	0.406
pilB	Vibrio cholerae strain A1552	38.272	100	0.403
pilB	Legionella pneumophila strain ERS1305867	37.219	100	0.395