Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RQT66_RS05130 Genome accession   NZ_CP135192
Coordinates   1036235..1036669 (+) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain 190055     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1031235..1041669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQT66_RS05100 tadA 1031345..1031845 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  RQT66_RS05105 - 1031860..1032711 (-) 852 WP_371694978.1 thymidylate synthase -
  RQT66_RS05110 lgt 1032708..1033520 (-) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  RQT66_RS05115 - 1033530..1034324 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RQT66_RS05120 rppH 1034327..1034923 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RQT66_RS05125 ampD 1035509..1036075 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RQT66_RS05130 pilA 1036235..1036669 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  RQT66_RS05135 pilB 1036669..1038054 (+) 1386 WP_014391364.1 GspE/PulE family protein Machinery gene
  RQT66_RS05140 - 1038054..1039265 (+) 1212 WP_079157761.1 type II secretion system F family protein -
  RQT66_RS05145 - 1039265..1039987 (+) 723 WP_041423102.1 A24 family peptidase -
  RQT66_RS05150 coaE 1040002..1040622 (+) 621 WP_014391361.1 dephospho-CoA kinase -
  RQT66_RS05155 yacG 1040633..1040836 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  RQT66_RS05160 - 1040836..1041105 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=886117 RQT66_RS05130 WP_005741096.1 1036235..1036669(+) (pilA) [Pasteurella multocida strain 190055]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=886117 RQT66_RS05130 WP_005741096.1 1036235..1036669(+) (pilA) [Pasteurella multocida strain 190055]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTTACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368