Detailed information NTG0886118

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: RQT66_RS05135
• Genome accession: NZ_CP135192
• Coordinates: 1036669..1038054 (+)
• Length: 461 a.a.
• NCBI ID: WP_014391364.1
• Uniprot ID: -
• Organism: Pasteurella multocida strain 190055
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1031669..1043054

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RQT66_RS05105	-	1031860..1032711 (-)	852	WP_371694978.1	thymidylate synthase	-
RQT66_RS05110	lgt	1032708..1033520 (-)	813	WP_014391365.1	prolipoprotein diacylglyceryl transferase	-
RQT66_RS05115	-	1033530..1034324 (-)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
RQT66_RS05120	rppH	1034327..1034923 (-)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RQT66_RS05125	ampD	1035509..1036075 (-)	567	WP_005719130.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RQT66_RS05130	pilA	1036235..1036669 (+)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RQT66_RS05135	pilB	1036669..1038054 (+)	1386	WP_014391364.1	GspE/PulE family protein	Machinery gene
RQT66_RS05140	-	1038054..1039265 (+)	1212	WP_079157761.1	type II secretion system F family protein	-
RQT66_RS05145	-	1039265..1039987 (+)	723	WP_041423102.1	A24 family peptidase	-
RQT66_RS05150	coaE	1040002..1040622 (+)	621	WP_014391361.1	dephospho-CoA kinase	-
RQT66_RS05155	yacG	1040633..1040836 (+)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
RQT66_RS05160	-	1040836..1041105 (+)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
RQT66_RS05165	-	1041554..1042927 (+)	1374	WP_005722571.1	sodium:alanine symporter family protein	-

Sequence

Protein

• Length: 461 a.a.
• Molecular weight: 52255.91 Da
• Isoelectric Point: 6.5608

>NTDB_id=886118 RQT66_RS05135 WP_014391364.1 1036669..1038054(+) (pilB) [Pasteurella multocida strain 190055]
MFHENPLCTVSTMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPHFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFTELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide

• Length: 1386 bp

>NTDB_id=886118 RQT66_RS05135 WP_014391364.1 1036669..1038054(+) (pilB) [Pasteurella multocida strain 190055]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTACAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAATATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCACTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTACTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGAAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (128-394)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (19-93)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Haemophilus influenzae 86-028NP	67.77	98.265	0.666
pilB	Haemophilus influenzae Rd KW20	67.329	98.265	0.662
pilB	Glaesserella parasuis strain SC1401	58.59	98.482	0.577
pilB	Acinetobacter baumannii D1279779	39.757	100	0.425
pilB	Acinetobacter baylyi ADP1	37.876	100	0.41
pilB	Vibrio parahaemolyticus RIMD 2210633	39.293	100	0.41
pilB	Vibrio campbellii strain DS40M4	39.04	100	0.406
pilB	Vibrio cholerae strain A1552	38.272	100	0.403
pilB	Legionella pneumophila strain ERS1305867	37.219	100	0.395