Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RQL82_RS16370 Genome accession   NZ_CP135159
Coordinates   3462746..3464458 (+) Length   570 a.a.
NCBI ID   WP_329759912.1    Uniprot ID   -
Organism   Acinetobacter pittii strain AR3651     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3457746..3469458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQL82_RS16335 (RQL82_16395) nusA 3458634..3460118 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  RQL82_RS16340 (RQL82_16400) rimP 3460156..3460680 (-) 525 WP_002114922.1 ribosome maturation factor RimP -
  RQL82_RS16360 (RQL82_16420) secG 3461318..3461647 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  RQL82_RS16365 (RQL82_16425) tpiA 3461660..3462454 (-) 795 WP_284091282.1 triose-phosphate isomerase -
  RQL82_RS16370 (RQL82_16430) pilB 3462746..3464458 (+) 1713 WP_329759912.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RQL82_RS16375 (RQL82_16435) pilC 3464486..3465712 (+) 1227 WP_329759913.1 type II secretion system F family protein Machinery gene
  RQL82_RS16380 (RQL82_16440) pilD 3465712..3466572 (+) 861 WP_276124893.1 prepilin peptidase Machinery gene
  RQL82_RS16385 (RQL82_16445) coaE 3466574..3467170 (+) 597 WP_276124892.1 dephospho-CoA kinase -
  RQL82_RS16390 (RQL82_16450) - 3467167..3468081 (-) 915 WP_329759916.1 DMT family transporter -
  RQL82_RS16395 (RQL82_16455) rlmB 3468117..3468866 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  RQL82_RS16400 (RQL82_16460) - 3468969..3469295 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63584.98 Da        Isoelectric Point: 5.0932

>NTDB_id=885753 RQL82_RS16370 WP_329759912.1 3462746..3464458(+) (pilB) [Acinetobacter pittii strain AR3651]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDISQI
PKDLVDQKLVLKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPSSQEEEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKTPADIPKQSLLEMGF
TDQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=885753 RQL82_RS16370 WP_329759912.1 3462746..3464458(+) (pilB) [Acinetobacter pittii strain AR3651]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGATGTTTACGATATAAGCCAAATT
CCCAAAGATTTGGTCGATCAGAAACTGGTTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCAACCAGCAACCCGAGTAATATTGAAGCGATTGATGCCATTCGCTTTAATAGCAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAGCAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCATCTTCACAAGAAGAGGAGGAAGAAACGCCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCATACGAGAAATCAT
ATCGGGTCCGTTACCGAGTCGATGGCGTGTTGCGTCAGATTGCAAATCCTCCTTTGCAATTGGCTAACCGTTTAGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAGCGGGTACCTCAAGATGGTCGTATCAAGCTTAAGTTATCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTATTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTCGATAAACCA
CAAGGTATGCTTCTAATTACAGGCCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGTTCGAATATTTCCACAGCCGAAGATCCAGTTGAAATTAACCTTGAAGGAATTAATCAGGTTAACGTCAACCCGA
AAGTAGGTCTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAAGATCCAGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACGGCTGAAATTGCAATTAAAGCTGCACAAACTGGTCATATGGTCATGTCCACACTACATACCAATAGTGC
TCCAGAAACACTAACTCGCCTACGCAATATGGGAGTTCCATCCTTTAATATCGCAACATCAGTCAACTTGGTTATTGCAC
AGCGTTTAGCACGTCGGCTATGCCCGCAGTGTAAAACACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACTGACCAAGATTTAACTCATCCCGATTTTCAAGTTTTCCAGCCTGTCGGTTGTACTGAGTGTCGTGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAGGAAGTCAATCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

77.895

100

0.779
  pilB Legionella pneumophila strain ERS1305867

51.146

99.474

0.509
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

51.132

92.982

0.475
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.096

91.053

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.016

100

0.377