Detailed information NTG0885142

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RPM48_RS27915
• Genome accession: NZ_CP135095
• Coordinates: 5969765..5970913 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain Paer3771
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5964765..5975913

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RPM48_RS27890 (RPM48_27890)	-	5965916..5966212 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
RPM48_RS27895 (RPM48_27895)	-	5966209..5966802 (-)	594	WP_003084544.1	YggT family protein	-
RPM48_RS27900 (RPM48_27900)	proC	5966813..5967634 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
RPM48_RS27905 (RPM48_27905)	-	5967646..5968338 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
RPM48_RS27910 (RPM48_27910)	pilT	5968553..5969587 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
RPM48_RS27915 (RPM48_27915)	pilU	5969765..5970913 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
RPM48_RS27920 (RPM48_27920)	czcD	5970921..5971820 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
RPM48_RS27925 (RPM48_27925)	-	5971975..5972379 (+)	405	WP_003084560.1	NINE protein	-
RPM48_RS27930 (RPM48_27930)	-	5972622..5973995 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
RPM48_RS27935 (RPM48_27935)	-	5973992..5975176 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=885142 RPM48_RS27915 WP_003100959.1 5969765..5970913(+) (pilU) [Pseudomonas aeruginosa strain Paer3771]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=885142 RPM48_RS27915 WP_003100959.1 5969765..5970913(+) (pilU) [Pseudomonas aeruginosa strain Paer3771]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382