Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ViNHUV68_RS02065 Genome accession   NZ_CP134975
Coordinates   410991..412682 (-) Length   563 a.a.
NCBI ID   WP_391089082.1    Uniprot ID   -
Organism   Vibrio sp. NH-UV-68     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 405991..417682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ViNHUV68_RS02035 (ViNHUV68_04110) rplS 406743..407096 (+) 354 WP_038173824.1 50S ribosomal protein L19 -
  ViNHUV68_RS02040 (ViNHUV68_04120) yacG 407203..407397 (-) 195 WP_391089076.1 DNA gyrase inhibitor YacG -
  ViNHUV68_RS02045 (ViNHUV68_04130) zapD 407463..408203 (-) 741 WP_391089078.1 cell division protein ZapD -
  ViNHUV68_RS02050 (ViNHUV68_04140) coaE 408238..408846 (-) 609 WP_038173829.1 dephospho-CoA kinase -
  ViNHUV68_RS02055 (ViNHUV68_04150) pilD 408848..409717 (-) 870 WP_391089080.1 prepilin peptidase Machinery gene
  ViNHUV68_RS02060 (ViNHUV68_04160) pilC 409755..410981 (-) 1227 WP_391090239.1 type II secretion system F family protein Machinery gene
  ViNHUV68_RS02065 (ViNHUV68_04170) pilB 410991..412682 (-) 1692 WP_391089082.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ViNHUV68_RS02070 (ViNHUV68_04180) - 412682..413086 (-) 405 WP_391089084.1 pilin -
  ViNHUV68_RS02075 (ViNHUV68_04190) nadC 413299..414186 (-) 888 WP_038173834.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ViNHUV68_RS02080 (ViNHUV68_04200) ampD 414318..414869 (+) 552 WP_391089086.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ViNHUV68_RS02085 (ViNHUV68_04210) fldB 414866..415387 (-) 522 WP_391089088.1 flavodoxin FldB -
  ViNHUV68_RS02090 (ViNHUV68_04220) xerD 415552..416448 (+) 897 WP_038173981.1 site-specific tyrosine recombinase XerD -
  ViNHUV68_RS02095 (ViNHUV68_04230) - 416488..417231 (+) 744 WP_038173838.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62686.89 Da        Isoelectric Point: 5.9168

>NTDB_id=884377 ViNHUV68_RS02065 WP_391089082.1 410991..412682(-) (pilB) [Vibrio sp. NH-UV-68]
MQSELLSLLVQLQLVSKQQQQRAQASIDELGQHPLTALIEDGVISDTELAKQLAEHLALPLIQLTEFDYQALSLPFDLRP
LIDRFQAVPVRLEQQTLQLAVADPCQHEIENEFHFATGFAIELVITEYSALINAIERLSQHDSTRSPLTDSPAINDHQLT
QLVTLSADEQREQDDLIHDKAPVSCYIQQILLDAVSRHASDIHFEPYEQSYRVRLRCDGLLISAQQPSHQLSRRLAARLK
ILAKLDIAERRLPQDGRIKLRLNDTMAVDMRVSTLPTLWGEKIVLRILDNRAIALAIDTLGFNPEQQHIYLSALKKPQGM
ILLTGPTGSGKTVSLYAALNHLNSQHVNISTAEDPVEINLPGINQVHIHPEIGLDFSHTLRAFLRQDPDIIMLGEIRDLE
TAEIAIKAAQTGHLVLSTLHTNSVVQTITRLANMGINHFNMASSLSLLIAQRLVRKLCRHCKQSIATPTLVAEQFHLAPN
SNMFIATPAGCPHCHNGYLGRIGIYELLPINAELVEAISNQATAEQLEAIAIENGMQSLIQSGIEKVRAGVTSIDELQRV
LYF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=884377 ViNHUV68_RS02065 WP_391089082.1 410991..412682(-) (pilB) [Vibrio sp. NH-UV-68]
ATGCAAAGCGAATTACTCAGCCTACTCGTTCAACTGCAACTCGTAAGCAAACAGCAGCAGCAACGCGCCCAAGCGAGCAT
CGACGAACTTGGCCAGCATCCGCTCACAGCCCTTATCGAAGATGGGGTTATCAGTGACACCGAGTTAGCTAAACAGCTGG
CCGAACACCTTGCGCTGCCCTTGATTCAACTCACCGAATTTGATTATCAAGCGCTCAGCCTTCCGTTCGACCTGCGGCCA
TTAATCGATCGCTTCCAAGCCGTTCCTGTTCGCCTTGAGCAGCAAACATTACAACTGGCCGTCGCTGACCCTTGCCAACA
CGAGATAGAAAATGAGTTTCATTTTGCTACTGGGTTCGCGATTGAATTGGTGATAACCGAATACAGTGCACTGATTAACG
CCATAGAGCGGCTATCACAGCATGATTCAACGCGCTCACCTCTGACTGACAGCCCTGCTATCAATGACCATCAACTCACC
CAATTAGTCACCTTGAGTGCCGATGAACAGCGTGAGCAAGATGACCTTATTCATGATAAAGCGCCCGTCAGTTGTTACAT
ACAACAGATCTTGTTAGATGCCGTCTCCCGCCACGCCTCTGACATTCACTTTGAACCATACGAACAGTCGTATCGAGTCC
GTTTACGTTGTGATGGTCTATTAATTTCCGCTCAGCAACCTTCACATCAACTCAGTCGCCGTTTAGCCGCTAGGCTGAAA
ATATTGGCCAAACTTGATATTGCCGAGCGCCGCCTACCGCAAGATGGCCGAATCAAGCTGCGTTTAAATGACACCATGGC
AGTGGATATGCGCGTATCCACCTTACCGACATTGTGGGGAGAAAAAATCGTCCTGCGTATTCTCGATAATCGCGCCATTG
CCCTCGCTATCGATACTTTAGGTTTTAACCCTGAGCAACAACACATCTACCTATCAGCGCTGAAAAAACCTCAAGGGATG
ATACTGCTCACCGGCCCAACCGGCAGTGGTAAAACCGTCTCGCTTTATGCGGCGCTCAATCACTTGAACTCACAGCATGT
TAATATCTCAACGGCAGAGGATCCGGTCGAAATTAATCTTCCCGGCATCAACCAAGTACACATTCACCCTGAAATTGGCC
TCGATTTTAGCCACACATTACGCGCGTTTTTACGCCAAGACCCAGACATCATCATGCTCGGCGAAATCCGCGACCTTGAG
ACAGCAGAGATCGCGATTAAAGCCGCTCAAACCGGCCACTTGGTACTGTCTACCTTGCACACCAATTCGGTGGTACAGAC
CATTACTCGCTTAGCCAATATGGGGATCAACCACTTTAATATGGCCTCTTCGCTCTCTTTACTTATCGCACAGCGCCTAG
TCAGGAAACTGTGCCGGCACTGCAAACAAAGCATCGCAACTCCAACTCTTGTCGCTGAACAGTTTCATCTCGCACCGAAC
TCGAATATGTTCATCGCCACCCCAGCAGGTTGCCCGCACTGTCACAATGGTTATCTCGGCCGAATAGGCATCTACGAGCT
GTTACCAATCAATGCCGAGCTTGTTGAAGCGATTTCAAATCAGGCCACAGCCGAGCAATTGGAAGCGATCGCGATAGAAA
ATGGTATGCAGAGTTTAATCCAATCTGGGATCGAAAAAGTGCGCGCAGGGGTTACCAGTATTGATGAGTTACAACGCGTA
CTCTACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

56.128

100

0.561
  pilB Vibrio parahaemolyticus RIMD 2210633

54.867

100

0.551
  pilB Vibrio campbellii strain DS40M4

53.805

100

0.54
  pilB Acinetobacter baylyi ADP1

45.841

100

0.46
  pilB Acinetobacter baumannii D1279779

45.552

99.822

0.455
  pilB Legionella pneumophila strain ERS1305867

46.241

94.494

0.437
  pilF Neisseria gonorrhoeae MS11

43.186

100

0.433
  pilF Thermus thermophilus HB27

36.691

98.757

0.362