Detailed information NTG0882864

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RJY17_RS03635
• Genome accession: NZ_CP134692
• Coordinates: 661628..662242 (-)
• Length: 204 a.a.
• NCBI ID: WP_064107157.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain B5
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 656628..667242

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RJY17_RS03605 (RJY17_03605)	rpsT	656931..657197 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
RJY17_RS03610 (RJY17_03610)	holA	657214..658255 (-)	1042	Protein_720	DNA polymerase III subunit delta	-
RJY17_RS03615 (RJY17_03615)	-	658295..658446 (-)	152	Protein_721	hypothetical protein	-
RJY17_RS03620 (RJY17_03620)	-	658487..658621 (+)	135	WP_003152870.1	YqzM family protein	-
RJY17_RS03625 (RJY17_03625)	comEC	658640..660991 (-)	2352	WP_060674655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RJY17_RS03630 (RJY17_03630)	-	660992..661561 (-)	570	WP_003152868.1	ComE operon protein 2	-
RJY17_RS03635 (RJY17_03635)	comEA	661628..662242 (-)	615	WP_064107157.1	helix-hairpin-helix domain-containing protein	Machinery gene
RJY17_RS03640 (RJY17_03640)	comER	662301..663122 (+)	822	WP_064107158.1	late competence protein ComER	-
RJY17_RS03645 (RJY17_03645)	-	663191..663928 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
RJY17_RS03650 (RJY17_03650)	rsfS	663925..664281 (-)	357	WP_007408260.1	ribosome silencing factor	-
RJY17_RS03655 (RJY17_03655)	yqeK	664299..664859 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RJY17_RS03660 (RJY17_03660)	-	664849..665418 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
RJY17_RS03665 (RJY17_03665)	yhbY	665429..665719 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
RJY17_RS03670 (RJY17_03670)	aroE	665713..666555 (-)	843	WP_033574150.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22249.47 Da
• Isoelectric Point: 6.4888

>NTDB_id=882864 RJY17_RS03635 WP_064107157.1 661628..662242(-) (comEA) [Bacillus velezensis strain B5]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=882864 RJY17_RS03635 WP_064107157.1 661628..662242(-) (comEA) [Bacillus velezensis strain B5]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.653	96.078	0.554
comEA	Lactococcus lactis subsp. cremoris KW2	35.135	100	0.382
comEA	Latilactobacillus sakei subsp. sakei 23K	33.19	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	36.493	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.493	100	0.377
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363