Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RHP75_RS18840 Genome accession   NZ_CP134499
Coordinates   3979347..3981434 (-) Length   695 a.a.
NCBI ID   WP_311091994.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20056     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3974347..3986434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP75_RS18825 (RHP75_18825) - 3976078..3977658 (-) 1581 WP_311089535.1 AAA family ATPase -
  RHP75_RS18830 (RHP75_18830) aroB 3977669..3978772 (-) 1104 WP_311089536.1 3-dehydroquinate synthase -
  RHP75_RS18835 (RHP75_18835) aroK 3978824..3979342 (-) 519 WP_311089537.1 shikimate kinase AroK -
  RHP75_RS18840 (RHP75_18840) pilQ 3979347..3981434 (-) 2088 WP_311091994.1 type IV pilus secretin PilQ Machinery gene
  RHP75_RS18845 (RHP75_18845) pilP 3981490..3982014 (-) 525 WP_409079750.1 type 4a pilus biogenesis lipoprotein PilP -
  RHP75_RS18850 (RHP75_18850) pilO 3982014..3982637 (-) 624 WP_311089539.1 type 4a pilus biogenesis protein PilO -
  RHP75_RS18855 (RHP75_18855) pilN 3982634..3983206 (-) 573 WP_311089540.1 type 4a pilus biogenesis protein PilN -
  RHP75_RS18860 (RHP75_18860) pilM 3983206..3984270 (-) 1065 WP_311089541.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74749.88 Da        Isoelectric Point: 4.9801

>NTDB_id=880582 RHP75_RS18840 WP_311091994.1 3979347..3981434(-) (pilQ) [Pseudomonas sp. SG20056]
MNISPSFIGLPLLAALLSPSLMAADLMALDVAALPGDRVELKLTFDEPVLAPRGYTIEQPARIALDLPGVSNKLGVKNRE
LGVGNARSVTVVEAKDRTRLVINLSSLSPYHTRAEGSSLFVVVGESSKIPTAAVGSAVAKFVAPAKKYSPPVRGISNIDF
QRGEQGEGNVVITLSDASVSPDIQDQGGRIRLDFAKTQLPESLRVKLDVKDFATPVQYVSTTSASDKVSVVIESSGVYDY
LAYQADNKLTLSVKPLTQSEADKRKADSFAYAGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVTGNITLRLQNVPWD
QALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKASDMAKLFQSVTSADGTADD
RGSITVDDRTNSIIAYQTQARLDELRRIVSQLDVAVRQVMIEARIVEANVDYDKALGVNWRGARVGNSNFVVGGSGGNRV
GAPGYGQNPGVQNPTPSLGNFVDLGVDGATSGIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGSE
IPYQEASSSGASTTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPNFERALNGIPPIDKNEVNAKVLVSDGETIVIGGV
FSNTQTRSVDKVPFLGELPYVGRLFRRDSVQEQKSELLVFLTPRIMNNQAVAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=880582 RHP75_RS18840 WP_311091994.1 3979347..3981434(-) (pilQ) [Pseudomonas sp. SG20056]
ATGAACATATCCCCCTCTTTTATTGGCCTCCCTCTTCTTGCTGCGCTTCTTTCTCCGTCGTTGATGGCTGCTGATTTAAT
GGCGCTTGACGTTGCAGCTTTGCCGGGGGATCGCGTTGAATTGAAGCTGACTTTTGACGAGCCTGTGCTCGCTCCTCGTG
GTTATACAATCGAGCAGCCTGCACGAATTGCGCTTGACTTGCCCGGGGTTTCGAACAAGTTGGGTGTGAAGAATAGAGAG
CTCGGCGTTGGTAACGCTCGTAGTGTTACCGTGGTCGAGGCGAAAGATCGTACTCGGCTTGTAATTAACCTATCGAGTCT
TTCCCCATATCACACTCGCGCAGAGGGGAGCAGTCTCTTTGTTGTGGTTGGTGAGAGCTCTAAAATTCCGACTGCTGCGG
TCGGTTCTGCTGTTGCTAAGTTCGTTGCTCCAGCGAAGAAATACTCTCCACCAGTGAGGGGGATTAGCAATATAGACTTT
CAGCGTGGCGAGCAGGGTGAGGGTAATGTGGTTATAACCTTGTCTGATGCGTCTGTAAGCCCTGATATACAAGATCAAGG
TGGTAGAATTCGCTTAGATTTTGCCAAGACACAGTTGCCAGAGTCTCTTCGTGTAAAGCTCGATGTGAAAGACTTTGCTA
CTCCGGTGCAATATGTAAGTACTACTAGTGCATCTGATAAGGTTAGCGTTGTCATTGAGTCGTCTGGTGTTTATGACTAC
TTGGCATACCAGGCTGATAATAAATTGACTTTAAGCGTTAAGCCGTTGACTCAGTCTGAGGCAGATAAGCGTAAAGCCGA
CAGCTTTGCCTATGCCGGCGAGAAGCTTTCTCTTAACTTTCAAGATATCGATGTTCGATCGGTTTTGCAATTGATCGCGG
ATTTCACGGACTTGAACTTGGTCGCTAGTGACACTGTTACTGGAAATATTACACTTCGGTTGCAGAATGTTCCGTGGGAT
CAGGCACTAGACCTAGTTCTGAAAACGAAGGGTCTAGATAAGCGCAAGATTGGTAATGTGCTTCTTGTGGCCCCTGCAGA
TGAAATAGCTGCGCGTGAAAGACAGGAGCTTGAGTCACAAAAGCAGATTGCTGAGCTGGCGCCTTTGCGTCGTGAGCTGA
TTCAGGTGAATTATGCCAAGGCGTCCGATATGGCAAAGCTTTTTCAGTCAGTGACAAGTGCAGATGGTACAGCGGATGAT
CGCGGATCTATAACGGTCGATGATAGAACTAACAGTATTATTGCCTACCAAACACAAGCTCGTTTAGATGAACTTCGTCG
TATAGTTTCCCAGCTTGATGTTGCTGTGCGTCAGGTTATGATTGAGGCGAGAATTGTTGAGGCCAACGTTGACTACGATA
AAGCGCTTGGAGTCAATTGGCGTGGTGCGCGTGTCGGTAATAGCAATTTTGTCGTTGGTGGTAGCGGTGGTAATCGTGTC
GGGGCTCCTGGTTATGGCCAGAACCCGGGTGTTCAAAATCCTACTCCATCGCTTGGTAATTTTGTGGATCTTGGCGTTGA
TGGGGCTACATCGGGGATTGGCATCGGCTTTATAACAGACAATACTATTTTAGATCTTGAGCTCTCTGCTATGGAGAAAA
CAGGTAATGGTGAAATTGTATCCCAGCCTAAAGTAGTAACTTCTGATAAGGAAACGGCCAAGATTCTTAAGGGGTCAGAG
ATTCCTTATCAGGAAGCGAGTTCTAGTGGTGCTAGCACAACGTCGTTTAAAGAAGCGGCTCTCTCTTTAGAGGTTACACC
TCAAATTACGCCGGATAATCGTATAATCATGGAGGTAAAGGTAACAAAAGATGCGCCGAACTTTGAACGCGCGCTCAATG
GTATTCCTCCTATCGATAAGAATGAGGTGAATGCGAAAGTTTTAGTAAGTGATGGCGAGACCATTGTAATTGGTGGTGTG
TTTTCCAATACTCAAACAAGGTCTGTTGATAAAGTCCCTTTCCTAGGTGAGCTCCCTTACGTAGGTCGGCTATTCAGGCG
TGATAGTGTTCAAGAGCAAAAATCCGAACTGCTTGTTTTCCTGACTCCACGCATCATGAACAACCAAGCCGTTGCGGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(39-121)

Secretin and TonB N terminus short domain (STN)

(299-345)

Bacterial type II/III secretion system short domain (Secretin_N)

(373-438)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.992

100

0.765
  comQ Acinetobacter baylyi ADP1

42.464

99.281

0.422
  pilQ Acinetobacter baumannii strain A118

41.595

100

0.42
  pilQ Acinetobacter baumannii D1279779

41.595

100

0.42
  pilQ Legionella pneumophila strain ERS1305867

37.519

96.259

0.361
  pilQ Legionella pneumophila strain Lp02

37.519

96.259

0.361