Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RHP75_RS18860 Genome accession   NZ_CP134499
Coordinates   3983206..3984270 (-) Length   354 a.a.
NCBI ID   WP_311089541.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20056     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3978206..3989270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP75_RS18835 (RHP75_18835) aroK 3978824..3979342 (-) 519 WP_311089537.1 shikimate kinase AroK -
  RHP75_RS18840 (RHP75_18840) pilQ 3979347..3981434 (-) 2088 WP_311091994.1 type IV pilus secretin PilQ Machinery gene
  RHP75_RS18845 (RHP75_18845) pilP 3981490..3982014 (-) 525 WP_409079750.1 type 4a pilus biogenesis lipoprotein PilP -
  RHP75_RS18850 (RHP75_18850) pilO 3982014..3982637 (-) 624 WP_311089539.1 type 4a pilus biogenesis protein PilO -
  RHP75_RS18855 (RHP75_18855) pilN 3982634..3983206 (-) 573 WP_311089540.1 type 4a pilus biogenesis protein PilN -
  RHP75_RS18860 (RHP75_18860) pilM 3983206..3984270 (-) 1065 WP_311089541.1 pilus assembly protein PilM Machinery gene
  RHP75_RS18865 (RHP75_18865) - 3984455..3986869 (+) 2415 WP_409079677.1 penicillin-binding protein 1A -
  RHP75_RS18870 (RHP75_18870) - 3986966..3988234 (-) 1269 WP_311089543.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37823.23 Da        Isoelectric Point: 4.4612

>NTDB_id=880583 RHP75_RS18860 WP_311089541.1 3983206..3984270(-) (pilM) [Pseudomonas sp. SG20056]
MLGLFNKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTVAV
AVAGSAVITKTIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGTSARNADRVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLIDQLGGGRDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVARSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FAEMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=880583 RHP75_RS18860 WP_311089541.1 3983206..3984270(-) (pilM) [Pseudomonas sp. SG20056]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATACGCTACTCGGGATCGATATCAGTTCGACCTCCGTCAAGCTCCTCGAATT
GAGCCGCTCCGGAAGCCGCTACAAGGTAGAGGCTTACGCAGTCGAGCCGCTCCCGCCAAACGCGGTGGTCGAAAAGAACA
TTGCCGAGCTGGAAGGGGTGGGGCAGGCCCTTTCGCGAGTACTCGCCAAAGCCAAGAGCGGCGTGAAAACCGTTGCAGTG
GCGGTGGCTGGGTCGGCAGTGATTACCAAAACCATCGAAATGGAAGCTGGGCTTTCCGACGATGAGCTTGAGAATCAGCT
GAAAATCGAAGCTGATCAGTACATTCCCTATCCGCTGGAAGAAGTCGCGATCGACTTCGAAGTCCAGGGGACCTCTGCGC
GTAATGCCGATCGTGTCGAAGTTCTGCTTGCCGCTTGTCGTAAGGAGAACGTTGAGGTCCGCGAGGCAGCTCTAGCGCTC
GCCGGTCTTACTGCCAAAGTGGTTGATGTCGAGGCCTATGCGCTTGAGCGGGCTTACGGCTTGTTGATTGATCAGCTCGG
TGGCGGTCGTGATGAGCTGACGGTTGCGGTTGTCGATATTGGCGCAACCATGACCACGCTCAGCGTTTTGCACAACGGTC
GCACTATCTACACCCGCGAGCAGCTGTTTGGCGGCAAGCAACTTACCGAAGAAATTCAGCGTCGTTATGGTCTCTCCGTT
GAAGAAGCTGGCCTTGCTAAGAAGCAAGGCGGCTTGCCGGATGACTATGACAGTGAAGTACTGCAGCCATTCAAAGAGGC
TGTGGTTCAGCAGGTTGCTCGTTCATTGCAGTTCTTCTTTGCTGCCGGTCAGTTCAATGATGTCGACTACATCTTGCTGG
CTGGTGGTACCGCGTCGATTCCCGATCTCGATCGTTTGATTCAGCAAAAAATAGGCACCCAGACATTGGTCGCCAATCCG
TTCGCTGAAATGGCGCTGAGTGGCAAGGTCAACGCAGGTGCGCTTGCCAGTGATGCGCCTGCCTTGATGATTGCCTGTGG
CCTGGCGATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.062

100

0.571
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463