Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RHP74_RS02395 Genome accession   NZ_CP134498
Coordinates   509632..511713 (+) Length   693 a.a.
NCBI ID   WP_311097615.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504632..516713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP74_RS02375 (RHP74_02375) comM 506844..507908 (+) 1065 WP_010464432.1 pilus assembly protein PilM Machinery gene
  RHP74_RS02380 (RHP74_02380) - 507908..508474 (+) 567 WP_010464430.1 PilN domain-containing protein -
  RHP74_RS02385 (RHP74_02385) pilO 508471..509094 (+) 624 WP_311097613.1 type 4a pilus biogenesis protein PilO -
  RHP74_RS02390 (RHP74_02390) - 509091..509618 (+) 528 WP_010464425.1 pilus assembly protein PilP -
  RHP74_RS02395 (RHP74_02395) pilQ 509632..511713 (+) 2082 WP_311097615.1 type IV pilus secretin PilQ Machinery gene
  RHP74_RS02400 (RHP74_02400) aroK 511718..512236 (+) 519 WP_010464421.1 shikimate kinase AroK -
  RHP74_RS02405 (RHP74_02405) aroB 512388..513488 (+) 1101 WP_102593630.1 3-dehydroquinate synthase -
  RHP74_RS02410 (RHP74_02410) - 513499..515097 (+) 1599 WP_311097616.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75055.36 Da        Isoelectric Point: 6.1153

>NTDB_id=880530 RHP74_RS02395 WP_311097615.1 509632..511713(+) (pilQ) [Pseudomonas sp. SG20052]
MNRIFSTLGISLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTESPARIALDLPGVVNQLSSKSRE
LGGGNARSATVVEAKDRTRLIINLTQLAPYSARVDGNNLIVVVGQGANNKTPRPTTRAPRAATAMPAPTKAYAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFAKTKLPEPLRVRLDVKDFATPVQFVNASASGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQISELAPLRRELLQVNYAKAADIAKLFTSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDLGAAGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=880530 RHP74_RS02395 WP_311097615.1 509632..511713(+) (pilQ) [Pseudomonas sp. SG20052]
ATGAACAGGATTTTCTCAACCCTCGGTATCTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
AGCGCTGGATGTTGCTGCGCTGCCGGGTGATCGTGTCGAGTTGAAGTTGTCTTTCGATGGCCCGCCCCCACAACCTCACG
GTTATACGACCGAGTCGCCGGCACGAATCGCGCTGGATCTGCCCGGGGTGGTCAATCAGCTTTCGAGCAAGAGTCGTGAG
CTGGGTGGCGGCAATGCCCGTAGCGCGACGGTGGTGGAAGCCAAGGACCGGACGCGGCTAATCATCAATCTGACCCAGTT
GGCCCCGTACAGCGCCCGTGTCGACGGCAACAACCTGATTGTGGTGGTCGGGCAAGGCGCCAATAACAAAACGCCTCGGC
CGACTACCCGCGCGCCTCGCGCCGCGACTGCAATGCCCGCCCCGACGAAAGCCTATGCGCCCGTGGGCAAAGCCATTCGC
GGTGTGGATTTCCAGCGCGGTACGCAGGGTGAAGGCAATGTAGTGATTGATCTGTCGGATCCGTCCATCGCCCCGGACAT
CCAGGAGCGCGACGGAAAGATCATCCTGGGATTCGCCAAAACCAAATTGCCAGAACCGTTGCGCGTGCGCCTGGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGCCTCGGGGGATCGGGCCACGATCAGTATCGAGCCCAGCGGA
GCCTTCGATTATTCGACCTACCAGACCGACAACAAGCTGACGGTCAGCATTCGGCCAATGACCGTCGACGACCTGCAAAA
GCGCAACGCCGAACGTTTTGCCTACACCGGTGAAAAACTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGTTTGCAGAAC
GTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGATCGGTAACGTCCTGCTGGT
GGCGCCGGCCGATGAAATCGCCGCGCGTGAACGTCAGGAACTGGAATCGCAGAAGCAGATTTCCGAGCTCGCGCCTTTGC
GTCGCGAATTGCTGCAGGTCAACTACGCCAAGGCCGCCGACATCGCCAAGCTGTTCACGTCGGTGACCAGTGCCGAAGCG
AAAATCGACGAGCGGGGTTCGATCACGGTTGATGAACGGACCAACAACATCATTGCCTACCAGACCCAGGACCGACTTGA
CGAACTTCGTCGCATCGTCGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGAATCGTCGAAGCCAACG
TCGACTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACACATCCGGCGTCAGC
AATGGCTCGTCGACCACTATCGGTACTCCGGGCAGCACCAGCACCAACTCGCCGTTTGTCGACCTGGGCGCTGCGGGCAA
CACCTCGGGGATCGGCATCGCCTTCATCACCGATAACGTGTTGCTGGACCTGGAGCTGACCGCAATGGAGAAGACCGGTA
ACGGCGAAATCGTCTCACAACCCAAGGTGGTCACGTCCGACAAGGAGACCGCGAAAATCCTCAAGGGCACCGAGATTCCC
TATCAGGAAGCCAGTTCCAGCGGTGCCACGTCGGTGTCGTTCAAAGAAGCGTCGCTGTCCCTTGAGGTCACTCCGCAGAT
CACCCCGGACAACCGGATCATCATGGAGGTCAAGGTGACCAAGGATGAGCCGGATTACCTGAACAAGGTCCAGGATGTAC
CCCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATCGTGATTGGTGGGGTTTTCTCA
AATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCATT
GA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.427

100

0.758
  comQ Acinetobacter baylyi ADP1

41.787

100

0.418
  pilQ Acinetobacter baumannii D1279779

38.329

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.329

100

0.417
  pilQ Legionella pneumophila strain Lp02

38.552

97.691

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.131

97.258

0.371