Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   RHP74_RS02375 Genome accession   NZ_CP134498
Coordinates   506844..507908 (+) Length   354 a.a.
NCBI ID   WP_010464432.1    Uniprot ID   A0A024EBZ8
Organism   Pseudomonas sp. SG20052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501844..512908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP74_RS02365 (RHP74_02365) - 502658..503926 (+) 1269 WP_010464435.1 malic enzyme-like NAD(P)-binding protein -
  RHP74_RS02370 (RHP74_02370) - 504182..506626 (-) 2445 WP_409201928.1 penicillin-binding protein 1A -
  RHP74_RS02375 (RHP74_02375) comM 506844..507908 (+) 1065 WP_010464432.1 pilus assembly protein PilM Machinery gene
  RHP74_RS02380 (RHP74_02380) - 507908..508474 (+) 567 WP_010464430.1 PilN domain-containing protein -
  RHP74_RS02385 (RHP74_02385) pilO 508471..509094 (+) 624 WP_311097613.1 type 4a pilus biogenesis protein PilO -
  RHP74_RS02390 (RHP74_02390) - 509091..509618 (+) 528 WP_010464425.1 pilus assembly protein PilP -
  RHP74_RS02395 (RHP74_02395) pilQ 509632..511713 (+) 2082 WP_311097615.1 type IV pilus secretin PilQ Machinery gene
  RHP74_RS02400 (RHP74_02400) aroK 511718..512236 (+) 519 WP_010464421.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38173.71 Da        Isoelectric Point: 4.6091

>NTDB_id=880529 RHP74_RS02375 WP_010464432.1 506844..507908(+) (comM) [Pseudomonas sp. SG20052]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPTNAVVEKNIAELEGVGQALARVLLKAKTNLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGVSVRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLAGQLAVSQEGLTVAVVDIGATMTTLSVLYNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVFRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=880529 RHP74_RS02375 WP_010464432.1 506844..507908(+) (comM) [Pseudomonas sp. SG20052]
GTGCTAGGACTCTTCAATAAAAAAGCCAATACGCTTCTGGGCATCGACATCAGCTCCACGTCGGTGAAGTTGCTTGAGCT
AAGTCGCCAGGGTGAGCGTTATCGGGTCGAGGCCTATGCGGTCGAGCCCTTGCCTACCAACGCAGTGGTCGAAAAAAATA
TCGCCGAGCTCGAAGGCGTGGGCCAGGCACTCGCGCGCGTGCTGCTCAAGGCCAAAACCAACCTCAGGAATGTCGCCGTG
GCCGTCGCCGGTTCGGCAGTCATCACCAAAACCATCGAGATGGACGCAGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCCTATCCATTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTGTCGGTGC
GTAACCCCGAACGGGTCAATGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTGGAGGTGCGTGAAGCCGCCCTGGCGCTG
GCCGGCCTGACCGCGCGAGTGGTGGATGTCGAGGCCTATGCGCTGGAGCGGTCGTTCTCCTTGCTGGCGGGGCAACTGGC
GGTCTCTCAGGAGGGGCTGACCGTGGCGGTGGTCGACATCGGTGCGACCATGACCACCCTGAGCGTGCTGTACAACGGGC
GGATCATCTACACCCGGGAACAATTGTTCGGCGGTCGTCAGCTCACGGAGGAAATCCAGCGTCGTTATGGCCTGACCGTC
GAGCAGGCGGGGTTGGCGAAGAAGCAGGGCGGGTTGCCGGATGACTACGTCAGCGAAGTCTTGCAGCCGTTTCGCGATGC
GCTGGTGCAGCAGGTTTTCCGGTCGTTGCAGTTCTTCTTCGCATCCGGTCAGTACAACGCGGTCGATCACATCCTGCTGG
CTGGCGGTACCGCGTCGGTCCCGGGGCTTGACCGCCTGATCGAGCAGCGGTTGGGCACGCCAACCCAGGTGGCCAATCCG
TTCTCCGACATGGCATTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCAAGCGATGCGCCAGCACTGATGATCGCTTGCGG
GCTCGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A024EBZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.627

100

0.576
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483