Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RJD40_RS12995 Genome accession   NZ_CP134280
Coordinates   2880892..2881350 (+) Length   152 a.a.
NCBI ID   WP_065430953.1    Uniprot ID   A0A1B1NRX3
Organism   Vibrio scophthalmi strain 21FBVib0106     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2875892..2886350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD40_RS12980 (RJD40_12980) recA 2876234..2877274 (-) 1041 WP_005593985.1 recombinase RecA Machinery gene
  RJD40_RS12985 (RJD40_12985) pncC 2877489..2877977 (-) 489 WP_418113552.1 nicotinamide-nucleotide amidase -
  RJD40_RS12990 (RJD40_12990) mutS 2878062..2880629 (+) 2568 WP_418113553.1 DNA mismatch repair protein MutS -
  RJD40_RS12995 (RJD40_12995) pilA 2880892..2881350 (+) 459 WP_065430953.1 pilin Machinery gene
  RJD40_RS13000 (RJD40_13000) pilB 2881350..2883032 (+) 1683 WP_418113554.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RJD40_RS13005 (RJD40_13005) pilC 2883086..2884309 (+) 1224 WP_065430955.1 type II secretion system F family protein Machinery gene
  RJD40_RS13010 (RJD40_13010) pilD 2884415..2885284 (+) 870 WP_005593978.1 prepilin peptidase Machinery gene
  RJD40_RS13015 (RJD40_13015) coaE 2885289..2885897 (+) 609 WP_005593977.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15721.86 Da        Isoelectric Point: 4.6853

>NTDB_id=879650 RJD40_RS12995 WP_065430953.1 2880892..2881350(+) (pilA) [Vibrio scophthalmi strain 21FBVib0106]
MNNTLTRSQRGFTLIELMVVMAIVAILAVFAVPAYQDYTKKATMAEFPTIAAPVKLAVEMCALEKASSTSTFATNCSTSA
GHVPSAAINNISVTATNVSGAIQVIAQATSDKGPVKSGEEFILSATYSDQGITWAASCTDAGGNAQSAYCPD

Nucleotide


Download         Length: 459 bp        

>NTDB_id=879650 RJD40_RS12995 WP_065430953.1 2880892..2881350(+) (pilA) [Vibrio scophthalmi strain 21FBVib0106]
ATGAACAATACGTTAACTCGCTCTCAACGCGGTTTCACTCTTATTGAATTAATGGTCGTCATGGCGATTGTCGCTATATT
GGCGGTGTTCGCGGTTCCTGCCTATCAAGACTACACCAAGAAAGCGACCATGGCGGAGTTTCCTACCATCGCCGCCCCAG
TAAAGCTAGCCGTTGAAATGTGCGCACTGGAAAAAGCCTCAAGTACCAGCACGTTTGCCACTAACTGCAGCACTAGTGCA
GGACATGTACCAAGCGCGGCAATCAACAACATCAGTGTTACCGCGACGAATGTCTCTGGCGCAATACAAGTGATCGCTCA
AGCAACAAGCGATAAAGGCCCGGTGAAATCTGGGGAAGAGTTCATTCTCAGCGCGACGTACAGCGATCAGGGCATTACTT
GGGCGGCCAGTTGTACAGACGCCGGTGGCAATGCACAAAGCGCCTACTGTCCAGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B1NRX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

52.597

100

0.533
  pilA Haemophilus influenzae Rd KW20

38.095

96.711

0.368
  pilA Haemophilus influenzae 86-028NP

38.732

93.421

0.362
  pilA2 Legionella pneumophila strain ERS1305867

38.194

94.737

0.362