Detailed information NTG0879653

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RJD40_RS13010
• Genome accession: NZ_CP134280
• Coordinates: 2884415..2885284 (+)
• Length: 289 a.a.
• NCBI ID: WP_005593978.1
• Uniprot ID: F9RL44
• Organism: Vibrio scophthalmi strain 21FBVib0106
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2879415..2890284

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RJD40_RS12995 (RJD40_12995)	pilA	2880892..2881350 (+)	459	WP_065430953.1	pilin	Machinery gene
RJD40_RS13000 (RJD40_13000)	pilB	2881350..2883032 (+)	1683	WP_418113554.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RJD40_RS13005 (RJD40_13005)	pilC	2883086..2884309 (+)	1224	WP_065430955.1	type II secretion system F family protein	Machinery gene
RJD40_RS13010 (RJD40_13010)	pilD	2884415..2885284 (+)	870	WP_005593978.1	prepilin peptidase	Machinery gene
RJD40_RS13015 (RJD40_13015)	coaE	2885289..2885897 (+)	609	WP_005593977.1	dephospho-CoA kinase	-
RJD40_RS13020 (RJD40_13020)	zapD	2885932..2886672 (+)	741	WP_005593976.1	cell division protein ZapD	-
RJD40_RS13025 (RJD40_13025)	yacG	2886748..2886942 (+)	195	WP_005593974.1	DNA gyrase inhibitor YacG	-
RJD40_RS13030 (RJD40_13030)	rplS	2887239..2887592 (-)	354	WP_005593972.1	50S ribosomal protein L19	-
RJD40_RS13035 (RJD40_13035)	trmD	2887642..2888391 (-)	750	WP_009387605.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
RJD40_RS13040 (RJD40_13040)	rimM	2888419..2888973 (-)	555	WP_005593970.1	ribosome maturation factor RimM	-
RJD40_RS13045 (RJD40_13045)	rpsP	2888998..2889246 (-)	249	WP_005593968.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32383.67 Da
• Isoelectric Point: 7.1964

>NTDB_id=879653 RJD40_RS13010 WP_005593978.1 2884415..2885284(+) (pilD) [Vibrio scophthalmi strain 21FBVib0106]
MELFVYYPWLFPLLATVFGLIVGSFLNVVIYRLPIMMEREWRHDCAESFPEYKIEVPQETFNLSVPRSACPSCHTQIRAI
DNIPVISWLLLKGKCHNCQSKISARYPLIELLTGGLCLLVATTLPASYFTIALLFFTFVLVAATFIDLDTMLLPDSLTLP
LMWSGIALALFGISPVSLQDSVIGAMAGYLCLWSVYWAFKLITKKEGMGYGDFKLLAALGAWLGWQYLPMIILVSSLVGL
VFGLIQLRLQSKGIDKAFPFGPYLAIAGWIAMLWGEQILHGYYRFLLGA

Nucleotide

• Length: 870 bp

>NTDB_id=879653 RJD40_RS13010 WP_005593978.1 2884415..2885284(+) (pilD) [Vibrio scophthalmi strain 21FBVib0106]
ATGGAACTGTTTGTTTATTACCCTTGGCTCTTCCCACTTCTTGCGACGGTGTTTGGTCTAATCGTTGGCAGCTTTCTCAA
TGTGGTCATCTACCGCTTACCGATTATGATGGAGCGCGAGTGGCGTCACGATTGTGCAGAAAGTTTTCCCGAATACAAAA
TTGAAGTTCCACAAGAGACATTTAACCTCAGCGTCCCACGATCAGCTTGCCCGAGTTGCCATACCCAGATTCGCGCCATC
GATAACATTCCTGTTATCAGCTGGCTACTGCTAAAAGGCAAATGTCACAACTGCCAAAGCAAAATCAGTGCTCGCTACCC
TCTGATCGAGTTGCTCACGGGGGGCTTGTGCTTGCTGGTTGCCACCACCTTACCCGCCAGTTATTTCACTATCGCGCTGC
TGTTTTTTACCTTCGTTTTAGTCGCAGCAACGTTTATCGATCTTGATACCATGTTGCTGCCTGATAGCCTGACTCTGCCA
CTAATGTGGAGCGGTATTGCCTTAGCCCTGTTTGGCATCAGTCCAGTGAGCTTGCAAGATTCGGTCATTGGCGCCATGGC
CGGCTATTTGTGCCTATGGAGTGTCTACTGGGCTTTCAAACTCATCACCAAAAAAGAAGGTATGGGTTACGGTGATTTTA
AATTGCTTGCCGCGCTAGGCGCCTGGCTTGGCTGGCAGTACCTGCCGATGATCATCTTAGTCTCATCTTTGGTGGGATTG
GTGTTTGGTCTTATTCAATTACGTCTACAGAGCAAAGGCATTGATAAAGCGTTTCCATTTGGTCCTTACTTAGCCATTGC
TGGTTGGATCGCCATGTTATGGGGGGAGCAGATCCTCCATGGCTACTACCGTTTTCTACTAGGAGCGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: F9RL44

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.083	99.654	0.768
pilD	Vibrio cholerae strain A1552	70.139	99.654	0.699
pilD	Neisseria gonorrhoeae MS11	53.16	93.08	0.495
pilD	Acinetobacter nosocomialis M2	53.008	92.042	0.488
pilD	Acinetobacter baumannii D1279779	52.632	92.042	0.484