Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RJD39_RS10165 Genome accession   NZ_CP134277
Coordinates   2229872..2231554 (+) Length   560 a.a.
NCBI ID   WP_406732018.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain 21FBVib0096     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2224872..2236554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD39_RS10150 (RJD39_10140) pncC 2226011..2226499 (-) 489 WP_069447145.1 nicotinamide-nucleotide amidase -
  RJD39_RS10155 (RJD39_10145) mutS 2226584..2229151 (+) 2568 WP_406732016.1 DNA mismatch repair protein MutS -
  RJD39_RS10160 (RJD39_10150) pilA 2229414..2229872 (+) 459 WP_009387600.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RJD39_RS10165 (RJD39_10155) pilB 2229872..2231554 (+) 1683 WP_406732018.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RJD39_RS10170 (RJD39_10160) pilC 2231608..2232834 (+) 1227 WP_406732019.1 type II secretion system F family protein Machinery gene
  RJD39_RS10175 (RJD39_10165) pilD 2232940..2233809 (+) 870 WP_069447149.1 A24 family peptidase Machinery gene
  RJD39_RS10180 (RJD39_10170) coaE 2233814..2234422 (+) 609 WP_009387604.1 dephospho-CoA kinase -
  RJD39_RS10185 (RJD39_10175) zapD 2234457..2235197 (+) 741 WP_005593976.1 cell division protein ZapD -
  RJD39_RS10190 (RJD39_10180) yacG 2235272..2235466 (+) 195 WP_005593974.1 DNA gyrase inhibitor YacG -
  RJD39_RS10195 (RJD39_10185) rplS 2235734..2236087 (-) 354 WP_005593972.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61858.97 Da        Isoelectric Point: 6.7740

>NTDB_id=879599 RJD39_RS10165 WP_406732018.1 2229872..2231554(+) (pilB) [Vibrio scophthalmi strain 21FBVib0096]
MSHHLATLLSQQGLLAHSQVGQPPFTSNHPSGFDPSILEFISADKLAAVVSQLFKLPQVNIAHYEYRTVCEELGLSTLIQ
KHLSLPLAKTNGTLTLAVVDPTLDVIEDEFRFATGLQIELVVTDYNAIKKAIYALYGRSLQSTTTAKKEINALELAQLVN
VSEEEFGREEDLSLDDAPVSRYIHQILLEAIRKGASDIHFEPYEKHYRIRLRCDGLLIESHQPASQLGRRLSTRLKILAN
LDIAERRRPQDGRSKVKLNADTAVDMRISTLPTLWGEKVVLRLLESGINLLDIDRLGYSHQQKQHYLKALKQPQGLILFT
GPTGSGKTISLYSGLNLLNTTQVNIATAEDPVEINLSGINQVQIQPQIGFDFACALRSFLRQDPDIVMVGEIRDVETADI
AIKASQTGHLVLSTLHTNSAADTIVRLASMGIKTYDLAASLTLVMAQRLVRKLCAYCKEPAKRNIDAKDDLLGPVTDQQY
QANQHGCTQCNQGYQGRIGLFEVLPINAKLQQAILQCATAIEIEALAKSEGMMSLQQSGIAALNAGKTSRQELQRVLLFD

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=879599 RJD39_RS10165 WP_406732018.1 2229872..2231554(+) (pilB) [Vibrio scophthalmi strain 21FBVib0096]
ATGAGTCATCATTTAGCGACGCTGCTTTCTCAACAAGGCCTACTTGCTCACTCGCAAGTAGGCCAGCCACCTTTTACCTC
AAACCATCCTTCTGGCTTTGATCCGTCGATACTAGAATTCATTAGCGCAGATAAATTAGCCGCTGTGGTGAGCCAATTAT
TCAAACTGCCACAGGTTAATATTGCTCATTATGAGTATCGTACGGTTTGTGAAGAGCTGGGTTTGAGTACACTTATCCAA
AAACACCTTTCATTACCACTTGCCAAAACCAATGGCACGCTCACGCTGGCAGTGGTCGATCCGACACTGGATGTGATTGA
AGACGAGTTTCGTTTTGCGACTGGATTGCAGATTGAATTAGTGGTGACTGACTACAATGCTATCAAGAAGGCCATTTATG
CCCTCTATGGCCGAAGCCTTCAATCCACCACAACCGCGAAGAAAGAGATCAATGCCCTGGAACTGGCACAGCTAGTGAAT
GTCAGCGAAGAGGAGTTTGGTCGAGAAGAAGATTTGAGCCTAGATGACGCCCCCGTCAGCCGCTACATTCATCAGATATT
ACTCGAGGCGATACGCAAAGGTGCTTCTGATATTCATTTTGAACCGTATGAAAAGCATTACCGGATTCGCCTACGCTGCG
ATGGCCTACTCATAGAAAGCCACCAACCAGCTTCTCAACTCGGCCGCCGTCTCTCCACACGGTTGAAAATATTGGCCAAC
TTAGATATCGCTGAACGTCGACGACCGCAAGATGGCAGAAGCAAGGTGAAGCTAAATGCTGATACCGCGGTGGATATGCG
AATCTCGACGCTACCCACTTTATGGGGTGAAAAGGTGGTGCTGCGATTATTGGAAAGCGGTATCAACCTGCTTGATATTG
ACCGACTCGGCTATAGCCACCAGCAAAAACAGCACTACTTAAAAGCACTCAAACAACCACAAGGGCTGATATTATTTACA
GGCCCAACAGGCAGCGGTAAAACCATCTCACTCTATAGTGGGCTAAATCTTCTCAACACGACGCAAGTCAATATTGCCAC
CGCAGAAGATCCAGTAGAGATCAATCTCAGCGGTATCAATCAGGTCCAAATTCAACCGCAAATCGGCTTCGACTTCGCTT
GCGCACTGCGCTCTTTTCTTCGTCAAGATCCAGATATTGTTATGGTCGGCGAAATTCGTGATGTTGAAACCGCTGACATT
GCGATCAAAGCGTCACAAACGGGCCACTTGGTTCTTTCCACTTTGCACACCAACTCCGCAGCTGACACCATCGTTCGTTT
AGCGAGTATGGGGATTAAAACTTATGATCTTGCCGCCTCACTGACGCTGGTGATGGCCCAGCGCTTAGTCAGAAAACTCT
GCGCGTATTGCAAAGAGCCAGCAAAACGAAACATCGATGCTAAGGACGATTTACTCGGACCCGTCACTGATCAGCAGTAT
CAAGCCAATCAGCACGGTTGTACTCAATGTAATCAGGGTTATCAAGGCAGAATAGGCTTGTTTGAAGTGCTGCCAATAAA
CGCCAAACTGCAACAAGCGATATTGCAATGCGCGACTGCCATTGAGATTGAAGCGCTCGCCAAAAGTGAAGGCATGATGA
GCTTACAACAATCCGGTATTGCTGCACTCAACGCTGGCAAAACCAGTCGCCAAGAATTACAACGCGTGTTGCTATTTGAC
TAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(185-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

59.574

100

0.6
  pilB Vibrio campbellii strain DS40M4

57.904

100

0.582
  pilB Vibrio cholerae strain A1552

60.345

93.214

0.563
  pilB Legionella pneumophila strain ERS1305867

46.516

94.821

0.441
  pilB Acinetobacter baumannii D1279779

44.636

93.214

0.416
  pilB Acinetobacter baylyi ADP1

44.084

93.571

0.413
  pilF Neisseria gonorrhoeae MS11

43.378

93.036

0.404