Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RJD39_RS10160 Genome accession   NZ_CP134277
Coordinates   2229414..2229872 (+) Length   152 a.a.
NCBI ID   WP_009387600.1    Uniprot ID   A0A1E3WRP2
Organism   Vibrio scophthalmi strain 21FBVib0096     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2224414..2234872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD39_RS10145 (RJD39_10135) recA 2224756..2225796 (-) 1041 WP_005593985.1 recombinase RecA Machinery gene
  RJD39_RS10150 (RJD39_10140) pncC 2226011..2226499 (-) 489 WP_069447145.1 nicotinamide-nucleotide amidase -
  RJD39_RS10155 (RJD39_10145) mutS 2226584..2229151 (+) 2568 WP_406732016.1 DNA mismatch repair protein MutS -
  RJD39_RS10160 (RJD39_10150) pilA 2229414..2229872 (+) 459 WP_009387600.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  RJD39_RS10165 (RJD39_10155) pilB 2229872..2231554 (+) 1683 WP_406732018.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RJD39_RS10170 (RJD39_10160) pilC 2231608..2232834 (+) 1227 WP_406732019.1 type II secretion system F family protein Machinery gene
  RJD39_RS10175 (RJD39_10165) pilD 2232940..2233809 (+) 870 WP_069447149.1 A24 family peptidase Machinery gene
  RJD39_RS10180 (RJD39_10170) coaE 2233814..2234422 (+) 609 WP_009387604.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15748.89 Da        Isoelectric Point: 4.6853

>NTDB_id=879598 RJD39_RS10160 WP_009387600.1 2229414..2229872(+) (pilA) [Vibrio scophthalmi strain 21FBVib0096]
MNNTLTRSQRGFTLIELMVVMAIVAILAVFAVPAYQDYTKKATMAEFPTIAAPVKLAVEMCALEKASSTSTFATNCNTSA
GHVPSAAINNISVTATNVSGAIQVIAQATSDKGPVKSGEEFILSATYSDQGITWAASCTDAGGNAQSAYCPD

Nucleotide


Download         Length: 459 bp        

>NTDB_id=879598 RJD39_RS10160 WP_009387600.1 2229414..2229872(+) (pilA) [Vibrio scophthalmi strain 21FBVib0096]
ATGAACAATACGTTAACTCGCTCTCAACGCGGTTTTACTCTTATTGAATTAATGGTCGTCATGGCGATTGTCGCTATATT
GGCGGTGTTCGCGGTTCCTGCCTATCAAGACTACACCAAGAAAGCGACCATGGCGGAGTTTCCTACCATCGCCGCCCCAG
TAAAGCTAGCCGTTGAAATGTGCGCACTGGAAAAAGCCTCAAGTACCAGCACGTTTGCCACTAACTGCAACACCAGTGCA
GGACATGTACCAAGCGCGGCAATCAACAACATCAGTGTTACCGCGACGAATGTCTCTGGCGCAATACAAGTGATCGCTCA
AGCAACAAGCGATAAAGGCCCGGTGAAATCTGGGGAAGAGTTCATTCTCAGCGCGACGTACAGCGATCAGGGCATTACTT
GGGCGGCCAGTTGTACAGACGCCGGTGGCAATGCACAAAGCGCCTACTGTCCAGATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3WRP2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

52.597

100

0.533
  pilA Haemophilus influenzae Rd KW20

38.095

96.711

0.368
  pilA Haemophilus influenzae 86-028NP

38.732

93.421

0.362
  pilA2 Legionella pneumophila strain ERS1305867

38.194

94.737

0.362