Detailed information NTG0879601

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: RJD39_RS10175
• Genome accession: NZ_CP134277
• Coordinates: 2232940..2233809 (+)
• Length: 289 a.a.
• NCBI ID: WP_069447149.1
• Uniprot ID: A0A1E3WRP3
• Organism: Vibrio scophthalmi strain 21FBVib0096
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2227940..2238809

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RJD39_RS10160 (RJD39_10150)	pilA	2229414..2229872 (+)	459	WP_009387600.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
RJD39_RS10165 (RJD39_10155)	pilB	2229872..2231554 (+)	1683	WP_406732018.1	type IV-A pilus assembly ATPase PilB	Machinery gene
RJD39_RS10170 (RJD39_10160)	pilC	2231608..2232834 (+)	1227	WP_406732019.1	type II secretion system F family protein	Machinery gene
RJD39_RS10175 (RJD39_10165)	pilD	2232940..2233809 (+)	870	WP_069447149.1	A24 family peptidase	Machinery gene
RJD39_RS10180 (RJD39_10170)	coaE	2233814..2234422 (+)	609	WP_009387604.1	dephospho-CoA kinase	-
RJD39_RS10185 (RJD39_10175)	zapD	2234457..2235197 (+)	741	WP_005593976.1	cell division protein ZapD	-
RJD39_RS10190 (RJD39_10180)	yacG	2235272..2235466 (+)	195	WP_005593974.1	DNA gyrase inhibitor YacG	-
RJD39_RS10195 (RJD39_10185)	rplS	2235734..2236087 (-)	354	WP_005593972.1	50S ribosomal protein L19	-
RJD39_RS10200 (RJD39_10190)	trmD	2236137..2236886 (-)	750	WP_009387605.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
RJD39_RS10205 (RJD39_10195)	rimM	2236914..2237468 (-)	555	WP_005593970.1	ribosome maturation factor RimM	-
RJD39_RS10210 (RJD39_10200)	rpsP	2237493..2237741 (-)	249	WP_005593968.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32411.72 Da
• Isoelectric Point: 7.1964

>NTDB_id=879601 RJD39_RS10175 WP_069447149.1 2232940..2233809(+) (pilD) [Vibrio scophthalmi strain 21FBVib0096]
MELFVYYPWLFPLLATVFGLIVGSFLNVVIYRLPIMMEREWRHDCAESFPEYKIEVPQETFNLSVPRSACPSCHTQIRAI
DNIPVISWLLLKGKCHNCQSKISARYPLIELLTGGLCLLVATTLPVSYFTIALLFFTFVLVAATFIDLDTMLLPDSLTLP
LMWSGIALALFGISPVSLQDSVIGAMAGYLCLWSVYWAFKLITKKEGMGYGDFKLLAALGAWLGWQYLPMIILVSSLVGL
VFGLIQLRLQSKGIDKAFPFGPYLAIAGWIAMLWGEQILHGYYRFLLGA

Nucleotide

• Length: 870 bp

>NTDB_id=879601 RJD39_RS10175 WP_069447149.1 2232940..2233809(+) (pilD) [Vibrio scophthalmi strain 21FBVib0096]
ATGGAACTGTTTGTTTATTACCCTTGGCTCTTCCCACTTCTTGCGACGGTGTTTGGTCTAATCGTTGGCAGCTTTCTCAA
TGTGGTCATCTACCGCTTACCGATTATGATGGAGCGCGAGTGGCGTCACGATTGTGCAGAAAGTTTTCCCGAATACAAAA
TTGAAGTTCCACAAGAGACATTTAACCTCAGCGTCCCACGCTCAGCTTGCCCGAGTTGCCATACCCAGATTCGCGCCATC
GATAACATTCCTGTTATCAGCTGGCTACTGCTAAAAGGCAAATGCCACAATTGCCAAAGCAAAATCAGTGCTCGCTACCC
TCTGATCGAGTTGCTCACGGGGGGCTTGTGCTTGCTGGTTGCCACCACCTTGCCCGTCAGTTACTTCACTATCGCGCTGC
TGTTTTTTACCTTCGTTTTAGTCGCAGCGACGTTTATCGATCTCGATACCATGTTGCTGCCTGATAGCCTGACTCTGCCA
CTAATGTGGAGCGGCATTGCCTTAGCCCTGTTTGGCATCAGTCCAGTGAGCTTGCAAGATTCGGTCATTGGCGCCATGGC
CGGCTATTTGTGCCTATGGAGTGTCTACTGGGCTTTCAAACTCATCACCAAAAAGGAAGGTATGGGTTACGGTGATTTTA
AATTACTTGCCGCGCTAGGCGCTTGGCTTGGCTGGCAGTACCTGCCGATGATCATCTTAGTCTCATCTTTGGTGGGATTG
GTGTTTGGGCTTATTCAATTACGTCTACAGAGCAAAGGCATTGATAAAGCGTTTCCATTTGGTCCTTACTTAGCCATCGC
TGGTTGGATCGCAATGTTATGGGGGGAGCAGATCCTCCATGGCTACTACCGTTTTCTACTAGGAGCGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A1E3WRP3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.083	99.654	0.768
pilD	Vibrio cholerae strain A1552	70.139	99.654	0.699
pilD	Neisseria gonorrhoeae MS11	53.16	93.08	0.495
pilD	Acinetobacter nosocomialis M2	53.383	92.042	0.491
pilD	Acinetobacter baumannii D1279779	53.008	92.042	0.488