Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RG494_RS03505 Genome accession   NZ_CP134046
Coordinates   756816..757250 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain AKS2022-HT5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 751816..762250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG494_RS03475 - 752377..752646 (-) 270 WP_241951066.1 GNAT family N-acetyltransferase -
  RG494_RS03480 yacG 752646..752849 (-) 204 WP_241951067.1 DNA gyrase inhibitor YacG -
  RG494_RS03485 coaE 752860..753480 (-) 621 WP_241951068.1 dephospho-CoA kinase -
  RG494_RS03490 - 753495..754217 (-) 723 WP_241951069.1 A24 family peptidase -
  RG494_RS03495 - 754217..755428 (-) 1212 WP_241952331.1 type II secretion system F family protein -
  RG494_RS03500 pilB 755428..756816 (-) 1389 WP_241951070.1 GspE/PulE family protein Machinery gene
  RG494_RS03505 pilA 756816..757250 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  RG494_RS03510 ampD 757410..757976 (+) 567 WP_241951071.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RG494_RS03515 rppH 758562..759158 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  RG494_RS03520 - 759161..759955 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  RG494_RS03525 lgt 759965..760768 (+) 804 WP_241951072.1 prolipoprotein diacylglyceryl transferase -
  RG494_RS03530 - 760774..761625 (+) 852 WP_115097855.1 thymidylate synthase -
  RG494_RS03535 tadA 761652..762140 (+) 489 WP_241951073.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=878289 RG494_RS03505 WP_005741096.1 756816..757250(-) (pilA) [Pasteurella multocida strain AKS2022-HT5]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=878289 RG494_RS03505 WP_005741096.1 756816..757250(-) (pilA) [Pasteurella multocida strain AKS2022-HT5]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG
TTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368