Detailed information NTG0878288

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: RG494_RS03500
• Genome accession: NZ_CP134046
• Coordinates: 755428..756816 (-)
• Length: 462 a.a.
• NCBI ID: WP_241951070.1
• Uniprot ID: -
• Organism: Pasteurella multocida strain AKS2022-HT5
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 750428..761816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RG494_RS03470	-	750554..751927 (-)	1374	WP_241951065.1	sodium:alanine symporter family protein	-
RG494_RS03475	-	752377..752646 (-)	270	WP_241951066.1	GNAT family N-acetyltransferase	-
RG494_RS03480	yacG	752646..752849 (-)	204	WP_241951067.1	DNA gyrase inhibitor YacG	-
RG494_RS03485	coaE	752860..753480 (-)	621	WP_241951068.1	dephospho-CoA kinase	-
RG494_RS03490	-	753495..754217 (-)	723	WP_241951069.1	A24 family peptidase	-
RG494_RS03495	-	754217..755428 (-)	1212	WP_241952331.1	type II secretion system F family protein	-
RG494_RS03500	pilB	755428..756816 (-)	1389	WP_241951070.1	GspE/PulE family protein	Machinery gene
RG494_RS03505	pilA	756816..757250 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
RG494_RS03510	ampD	757410..757976 (+)	567	WP_241951071.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
RG494_RS03515	rppH	758562..759158 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
RG494_RS03520	-	759161..759955 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
RG494_RS03525	lgt	759965..760768 (+)	804	WP_241951072.1	prolipoprotein diacylglyceryl transferase	-
RG494_RS03530	-	760774..761625 (+)	852	WP_115097855.1	thymidylate synthase	-

Sequence

Protein

• Length: 462 a.a.
• Molecular weight: 52165.79 Da
• Isoelectric Point: 6.3686

>NTDB_id=878288 RG494_RS03500 WP_241951070.1 755428..756816(-) (pilB) [Pasteurella multocida strain AKS2022-HT5]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TVDQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide

• Length: 1389 bp

>NTDB_id=878288 RG494_RS03500 WP_241951070.1 755428..756816(-) (pilB) [Pasteurella multocida strain AKS2022-HT5]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CCACCCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCCGAGTTGGGGATG
ACCGTAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGGCTAATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTTTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (128-394)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (19-93)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Haemophilus influenzae 86-028NP	67.991	98.052	0.667
pilB	Haemophilus influenzae Rd KW20	67.329	98.052	0.66
pilB	Glaesserella parasuis strain SC1401	58.37	98.268	0.574
pilB	Acinetobacter baumannii D1279779	39.351	100	0.42
pilB	Vibrio parahaemolyticus RIMD 2210633	40.125	100	0.418
pilB	Vibrio campbellii strain DS40M4	39.419	100	0.411
pilB	Vibrio cholerae strain A1552	38.683	100	0.407
pilB	Acinetobacter baylyi ADP1	37.675	100	0.407
pilB	Legionella pneumophila strain ERS1305867	36.81	100	0.39