Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   RF668_RS09790 Genome accession   NZ_CP133787
Coordinates   1897252..1898121 (-) Length   289 a.a.
NCBI ID   WP_043734523.1    Uniprot ID   A0AAX4A4Q0
Organism   Lactococcus cremoris strain KCKM 0438     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1892252..1903121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RF668_RS09775 (RF668_09775) xerS 1892337..1893407 (+) 1071 WP_011835149.1 tyrosine recombinase XerS -
  RF668_RS09780 (RF668_09780) trmFO 1893457..1894803 (-) 1347 WP_043734520.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  RF668_RS09785 (RF668_09785) topA 1894875..1897007 (-) 2133 WP_043734521.1 type I DNA topoisomerase -
  RF668_RS09790 (RF668_09790) dprA 1897252..1898121 (-) 870 WP_043734523.1 DNA-processing protein DprA Machinery gene
  RF668_RS09795 (RF668_09795) - 1898269..1898649 (-) 381 WP_043734524.1 RidA family protein -
  RF668_RS09800 (RF668_09800) budA 1898689..1899399 (-) 711 WP_043734525.1 acetolactate decarboxylase -
  RF668_RS09805 (RF668_09805) ilvA 1899535..1900785 (-) 1251 WP_043734528.1 threonine ammonia-lyase IlvA -
  RF668_RS09810 (RF668_09810) ilvC 1900844..1901866 (-) 1023 WP_043734529.1 ketol-acid reductoisomerase -
  RF668_RS09815 (RF668_09815) ilvN 1901911..1902387 (-) 477 WP_021037263.1 acetolactate synthase small subunit -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32606.90 Da        Isoelectric Point: 10.0469

>NTDB_id=876892 RF668_RS09790 WP_043734523.1 1897252..1898121(-) (dprA) [Lactococcus cremoris strain KCKM 0438]
MITNFDLFRWKKAGMTNLGVNKLLKFFRKYDRKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRADYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIVTELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYNLKIAISP

Nucleotide


Download         Length: 870 bp        

>NTDB_id=876892 RF668_RS09790 WP_043734523.1 1897252..1898121(-) (dprA) [Lactococcus cremoris strain KCKM 0438]
ATGATTACAAATTTTGATTTATTTCGTTGGAAAAAAGCAGGAATGACCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
TCGCAAATATGATAGAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAAGATGTAAAAAAACTAAGAGCAGACTATAAAAAATTCTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGCCTAAGAGAAATCTATAATCCACCTGTCTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAATCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTGTGACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGGCTTGCTAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTTCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGAGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGAAGTTTAATCACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGACGGAACATCCGATGGATGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTTTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTTAAAAATAGCTATAAGCCCTTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)

DNA recombination-mediator protein A (DNA_processg_A)

(69-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

99.291

97.578

0.969
  dprA Streptococcus mutans UA159

58.993

96.194

0.567
  dprA/cilB/dalA Streptococcus mitis SK321

55.714

96.886

0.54
  dprA/cilB/dalA Streptococcus pneumoniae R6

55.357

96.886

0.536
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

55.357

96.886

0.536
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

55.357

96.886

0.536
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

55.357

96.886

0.536
  dprA/cilB/dalA Streptococcus pneumoniae D39

55.357

96.886

0.536
  dprA Bacillus subtilis subsp. subtilis str. 168

42.105

92.042

0.388
  dprA Vibrio campbellii strain DS40M4

35.644

100

0.374
  dprA Legionella pneumophila strain ERS1305867

39.13

95.502

0.374
  dprA Haemophilus influenzae Rd KW20

39.63

93.426

0.37