Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PszF2a_RS02960 Genome accession   NZ_AP024722
Coordinates   642383..644482 (+) Length   699 a.a.
NCBI ID   WP_126526950.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 637383..649482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PszF2a_RS02940 (PszF2a_05810) comM 639549..640613 (+) 1065 WP_126526953.1 pilus assembly protein PilM Machinery gene
  PszF2a_RS02945 (PszF2a_05820) - 640613..641185 (+) 573 WP_126526952.1 PilN domain-containing protein -
  PszF2a_RS02950 (PszF2a_05830) pilO 641182..641805 (+) 624 WP_019405681.1 type 4a pilus biogenesis protein PilO -
  PszF2a_RS02955 (PszF2a_05840) pilP 641802..642329 (+) 528 WP_126526951.1 type 4a pilus biogenesis lipoprotein PilP -
  PszF2a_RS02960 (PszF2a_05850) pilQ 642383..644482 (+) 2100 WP_126526950.1 type IV pilus secretin PilQ Machinery gene
  PszF2a_RS02965 (PszF2a_05860) aroK 644489..645034 (+) 546 WP_041755271.1 shikimate kinase AroK -
  PszF2a_RS02970 (PszF2a_05870) aroB 645058..646161 (+) 1104 WP_011911792.1 3-dehydroquinate synthase -
  PszF2a_RS02975 (PszF2a_05880) - 646172..647743 (+) 1572 WP_221292929.1 AAA family ATPase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75164.10 Da        Isoelectric Point: 4.7313

>NTDB_id=87684 PszF2a_RS02960 WP_126526950.1 642383..644482(+) (pilQ) [Stutzerimonas stutzeri strain F2a]
MNTTLSRLGVSLLAVFISPALLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGESSGTTSPAAASAAVTAPIAKPAPVKPMAAGKAIDN
IDFRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVVFAKAELPEALRVRLDVQDFATPVKFVDSTAQAGGASIAIEPTGR
YDYLAYQTDNKLTISVKPLTEEEADKRKTDRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTNNGGV
AEERGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLGAGNKWSAWGKNG
NLDIEDGEDGGQQITWPMNTPFVDMGAVGSTSGIGLGFVTDNAILDLQLSAMEKTGNGEVVSQPKVVTADKETAKILKGS
EVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVTDGETIVIG
GVFSNTQSKSVDKVPFLGDLPYLGRLFRRDLVQDRKSELLVFITPRIMNNQSIQASRSY

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=87684 PszF2a_RS02960 WP_126526950.1 642383..644482(+) (pilQ) [Stutzerimonas stutzeri strain F2a]
ATGAACACTACCCTTTCCCGTCTGGGCGTATCCCTGCTGGCGGTATTTATTTCGCCAGCACTGCTGGCGGCCAATCTCAA
CGCTTTGGATGTGGCTGCTTTGCCGGGCGATCGGGTTGAGTTGAAACTGGCATTTGACGAGCCTGTCGCGTCACCTCGTG
GCTACACGCTGGACCAGCCCGCGCGCATTGCCCTGGATCTTCCAGGTGTATCCAACAACCTGGGGGCTAAAAACCGAGAG
CTAGGCGTTGGTAATGCGCGCAGCGTAACGGTCGTCGAGGCTCAAGGGCGCACCCGGCTCATCGTCAATCTGACCGCGCT
TGTTCCGTATTCCACTCGAGTGGATGGGAACAATCTGTTTGTCGTGCTGGGAGAGAGCTCGGGGACGACCAGCCCTGCGG
CTGCATCCGCCGCGGTCACTGCTCCGATTGCCAAGCCTGCTCCGGTAAAGCCGATGGCGGCCGGCAAGGCGATCGACAAT
ATCGATTTTCGCCGCGGTGAAGATGGTGCCGGAAACGTGGTAATCACACTGTCCGATCCGTCGGTAGGTTCGACTATCGA
AGAGCAGGGAGGAAAGATCCGCGTTGTGTTCGCCAAGGCGGAGCTGCCTGAAGCGCTGCGTGTTCGGCTTGACGTCCAGG
ATTTCGCTACGCCGGTGAAGTTTGTCGACTCGACCGCTCAGGCAGGCGGTGCCAGTATCGCCATCGAGCCTACTGGGCGA
TACGATTATCTCGCCTACCAGACAGACAACAAGCTGACCATCAGCGTAAAGCCCCTTACAGAAGAAGAGGCCGATAAGCG
CAAGACAGACCGTTTTGCCTATTCCGGTGAAAAACTGTCGCTGAACTTCCAAGATATTGATGTTCGTTCCGTACTCCAGT
TGATCGCTGATTTTACTGACCTGAACTTGGTTGCCAGCGATACCGTCAGTGGCAACATCACCCTTCGTTTGCAGAACGTG
CCTTGGGACCAAGCACTCGACCTCGTTTTGAAAACCAAAGGGCTGGATAAGCGGCAAGTCGGTAACGTACTGCTCGTTGC
GCCCGCTGATGAAATCGCGGCACGTGAACGCCAAGAGCTTGAGTCGCAGCGCCAAATTGCCGAGCTTGCACCGTTGCGTC
GCGAGGTTGTGCAGGTCAACTATGCCAAGGCTGCGGACATTGCCCGCCTGTTTCAGTCGGTGACGAACAATGGCGGCGTG
GCGGAAGAGCGTGGATCGATTGCGGTGGACGACCGTACTAACAACATCATCGCTTATCAAACGCAAGAGCGGCTAGATGA
GTTGCGTCGTATTGTTGCTCAGCTCGATATCCCGGTGCGGCAGGTCATGATCGAAGCGCGGATCGTCGAGGCGAACGTTG
ACTACGACAAGAGTCTCGGCGTTCGCTGGGGGGGAAATCTTGGAGCTGGCAACAAATGGAGCGCTTGGGGAAAGAACGGC
AATCTGGATATTGAGGATGGTGAAGACGGCGGCCAACAGATTACATGGCCTATGAATACGCCGTTTGTAGATATGGGGGC
TGTCGGTTCTACTTCTGGCATCGGACTCGGATTTGTAACGGATAACGCGATCCTTGACCTCCAGCTTTCGGCGATGGAGA
AGACGGGAAATGGCGAGGTGGTTTCTCAGCCTAAAGTGGTTACTGCCGATAAGGAAACCGCAAAAATTCTCAAAGGCTCG
GAAGTTCCCTATCAGGAAGCTAGTTCCAGTGGGGCAACTAGCACATCCTTTAAAGAGGCTGCGCTTTCGCTAGAGGTGAC
GCCGCAGATTACGCCGGATAACCGAATCATCATGGAAGTAAAGGTCACAAAGGATGAGCCGGATTTTTCACAGGCAGCGA
CAACCGGCGTGCCGGCTATCCGCAAGAATGAAGTGAACGCGAAAGTGCTTGTCACTGATGGCGAGACGATTGTTATCGGC
GGAGTCTTCTCTAATACGCAAAGCAAATCGGTCGATAAGGTGCCATTCCTTGGGGATCTGCCATATCTTGGACGCCTTTT
CCGTCGCGACCTAGTGCAAGATCGAAAATCTGAGCTGCTTGTGTTCATTACTCCTAGGATCATGAATAATCAGTCAATCC
AAGCTAGCCGCTCATATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

Bacterial type II and III secretion system protein (Secretin)

(531-688)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.173

100

0.775
  pilQ Acinetobacter baumannii strain A118

39.892

100

0.423
  pilQ Acinetobacter baumannii D1279779

39.892

100

0.423
  comQ Acinetobacter baylyi ADP1

41.908

98.999

0.415
  pilQ Legionella pneumophila strain Lp02

37.518

96.853

0.363
  pilQ Legionella pneumophila strain ERS1305867

37.518

96.853

0.363

Multiple sequence alignment