Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RHM55_RS16900 Genome accession   NZ_CP133784
Coordinates   3608190..3610277 (+) Length   695 a.a.
NCBI ID   WP_322177456.1    Uniprot ID   -
Organism   Pseudomonas sp. MH9.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3603190..3615277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM55_RS16880 (RHM55_16880) comM 3605293..3606357 (+) 1065 WP_322177452.1 pilus assembly protein PilM Machinery gene
  RHM55_RS16885 (RHM55_16885) - 3606357..3606923 (+) 567 WP_322177453.1 PilN domain-containing protein -
  RHM55_RS16890 (RHM55_16890) pilO 3606920..3607543 (+) 624 WP_322177454.1 type 4a pilus biogenesis protein PilO -
  RHM55_RS16895 (RHM55_16895) - 3607540..3608079 (+) 540 WP_322177455.1 pilus assembly protein PilP -
  RHM55_RS16900 (RHM55_16900) pilQ 3608190..3610277 (+) 2088 WP_322177456.1 type IV pilus secretin PilQ Machinery gene
  RHM55_RS16905 (RHM55_16905) aroK 3610282..3610800 (+) 519 WP_322177457.1 shikimate kinase AroK -
  RHM55_RS16910 (RHM55_16910) aroB 3610930..3612033 (+) 1104 WP_322177458.1 3-dehydroquinate synthase -
  RHM55_RS16915 (RHM55_16915) - 3612043..3613653 (+) 1611 WP_322177459.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74701.26 Da        Isoelectric Point: 5.4199

>NTDB_id=876789 RHM55_RS16900 WP_322177456.1 3608190..3610277(+) (pilQ) [Pseudomonas sp. MH9.2]
MTRIFSVVGISLWIAMVSPMLQAANLKALDVAALPGDRVELKLTFDGPIPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGVGNARSVVVVEAKDRTRVIINMTVLAPYSSRVEGNNLFVVVGQGAASAQGIKPSAAPTSARVSVPAAAPARPYVAVGK
AIKNIDFQRGELGEGNVIIELSDPGIAPDIQEQGGKIRVNFAKTQLPDPLRVRLDVKDFATPVQFVNASVAGDKASISIE
PTGAFDYSAYQTDNKLTISVRPLTSEDLDRRNADKPIYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESMKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AETKADERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSSNRSGSNNAT
VGGKGTVTGANGVVTNSNSPFVDLGAANATSGIGIAFVTDNVLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYINTVLGVPPIKKNEVNAKVLVADGETIVIGGV
FSNTQSKIVDKVPFLGDVPYVGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=876789 RHM55_RS16900 WP_322177456.1 3608190..3610277(+) (pilQ) [Pseudomonas sp. MH9.2]
ATGACTAGGATTTTCTCGGTTGTCGGCATTTCGCTATGGATAGCGATGGTTTCGCCGATGCTCCAGGCGGCTAACTTGAA
AGCTCTGGATGTCGCCGCTTTGCCAGGAGATCGGGTCGAGTTGAAGCTGACCTTCGACGGACCAATCCCCGCGCCGCGCG
GCTATACCACTGAGCAACCCGCACGTATTGCTCTGGATTTGCCCGGTGTGACCAGCCAACTGGCGACCAAAAGTCGTGAT
TTGGGCGTGGGCAATGCTCGCAGTGTGGTGGTGGTCGAGGCCAAAGACCGCACGCGGGTCATTATCAATATGACGGTGCT
TGCGCCCTACAGCTCGCGGGTCGAGGGTAATAACCTGTTCGTGGTCGTGGGTCAGGGCGCAGCCTCTGCTCAGGGGATCA
AGCCAAGCGCGGCGCCCACGTCTGCACGGGTTTCAGTTCCGGCCGCAGCACCTGCCAGGCCCTATGTTGCCGTGGGAAAA
GCGATCAAGAACATCGATTTCCAGCGCGGTGAGTTGGGGGAAGGTAACGTCATCATCGAACTGAGTGACCCAGGCATCGC
CCCGGACATTCAGGAGCAAGGTGGCAAAATCCGGGTCAATTTCGCGAAAACCCAGCTCCCGGACCCCTTGCGCGTTCGGT
TGGATGTCAAGGATTTCGCCACGCCTGTGCAGTTCGTCAATGCCAGCGTAGCGGGTGACAAGGCCAGTATCAGCATCGAG
CCGACTGGCGCGTTTGATTATTCGGCCTATCAGACCGATAACAAACTGACCATCAGTGTGCGGCCGTTGACGTCCGAAGA
CCTGGATCGTCGAAATGCAGACAAGCCGATCTACACCGGAGAAAAGCTGTCGCTGAACTTCCAGGACATCGACGTTCGCT
CGGTCTTGCAGCTGATTGCCGATTTTACCAACCTCAATCTGGTGGCCAGCGACACAGTGCAGGGCGGGATCACCTTGCGC
TTGCAGAACGTGCCGTGGGATCAGGCGCTGGATCTGGTACTGAAAACCAAAGGACTGGATAAACGCAAGATCGGCAATGT
CCTGTTAGTCGCGCCTGCGGATGAAATCGCCGCTCGCGAACGTCAGGAACTGGAGTCGATGAAGCAGATTGCCGAGTTGG
CGCCGTTGCGCCGCGAGCTGCTGCAAGTGAATTACGCCAAGGCGGCCGACATCGCCAAGCTGTTCCAGTCGGTGACCAGC
GCTGAGACCAAAGCCGATGAGCGCGGCTCGATCACCGTCGATGACCGGACGAACAACATCATTGCCTACCAGACTCAGGA
CCGTCTCGATGAGCTGCGCAGGATTGTTTCGCAGCTGGATATCCCCGTGCGACAGGTGATGATCGAGGCACGCATCGTCG
AGGCCAACGTCGATTATGACAAGGCGCTGGGCGTGCGCTGGGGAGGTTCGAGCAATCGCAGCGGTAGCAACAATGCGACT
GTCGGCGGCAAAGGTACGGTGACCGGCGCCAATGGTGTCGTCACTAACTCCAACTCACCTTTCGTCGATCTGGGCGCTGC
CAACGCGACGTCCGGGATCGGTATCGCTTTCGTGACCGATAACGTGCTGCTGGACCTTGAGTTGAGTGCCATGGAGAAAA
CCGGTAACGGTGAGATCGTTTCCCAACCCAAGGTCGTGACCTCCGATAAAGAGACCGCGAAGATCCTCAAGGGGACTGAA
GTCCCGTATCAGGAAGCCAGTTCCAGTGGCGCGACGTCGGTGTCTTTCAAAGAGGCGTCGCTGTCCCTGGAGGTGACGCC
GCAAATCACCCCTGATAACCGCATTATCATGGAGGTCAAGGTTACCAAGGATGAGCCGGATTACATCAATACCGTGCTGG
GTGTACCGCCAATCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTGGCTGACGGCGAAACCATCGTCATCGGCGGTGTT
TTCTCTAATACGCAGAGTAAAATCGTCGACAAGGTGCCATTTCTTGGCGATGTGCCGTATGTTGGCCGGCTTTTCCGGCG
TGACGTTGTTTCAGAGAAAAAATCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(39-121)

Secretin and TonB N terminus short domain (STN)

(306-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.022

100

0.763
  comQ Acinetobacter baylyi ADP1

42.265

100

0.44
  pilQ Acinetobacter baumannii strain A118

39.488

100

0.422
  pilQ Acinetobacter baumannii D1279779

39.488

100

0.422
  pilQ Legionella pneumophila strain Lp02

39.883

98.129

0.391
  pilQ Legionella pneumophila strain ERS1305867

39.883

98.129

0.391