Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHM55_RS11000 Genome accession   NZ_CP133784
Coordinates   2356850..2358550 (+) Length   566 a.a.
NCBI ID   WP_322181950.1    Uniprot ID   -
Organism   Pseudomonas sp. MH9.2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2351850..2363550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM55_RS10980 (RHM55_10980) - 2352487..2354721 (-) 2235 WP_322181944.1 DUF1631 domain-containing protein -
  RHM55_RS10985 (RHM55_10985) nadC 2355057..2355905 (+) 849 WP_322181946.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHM55_RS10995 (RHM55_10995) - 2356197..2356628 (-) 432 WP_322181948.1 pilin -
  RHM55_RS11000 (RHM55_11000) pilB 2356850..2358550 (+) 1701 WP_322181950.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM55_RS11005 (RHM55_11005) pilC 2358553..2359770 (+) 1218 WP_322181952.1 type II secretion system F family protein Machinery gene
  RHM55_RS11010 (RHM55_11010) pilD 2359773..2360645 (+) 873 WP_322181954.1 A24 family peptidase Machinery gene
  RHM55_RS11015 (RHM55_11015) coaE 2360642..2361265 (+) 624 WP_322181956.1 dephospho-CoA kinase -
  RHM55_RS11020 (RHM55_11020) yacG 2361262..2361465 (+) 204 WP_322181958.1 DNA gyrase inhibitor YacG -
  RHM55_RS11025 (RHM55_11025) - 2361492..2362190 (-) 699 WP_322181961.1 energy-coupling factor ABC transporter permease -
  RHM55_RS11030 (RHM55_11030) - 2362187..2362648 (-) 462 WP_322181964.1 hypothetical protein -
  RHM55_RS11035 (RHM55_11035) - 2362752..2363186 (-) 435 WP_322181966.1 DUF393 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62345.81 Da        Isoelectric Point: 6.5142

>NTDB_id=876773 RHM55_RS11000 WP_322181950.1 2356850..2358550(+) (pilB) [Pseudomonas sp. MH9.2]
MTDVVLTGLAKQLVLTNLLNEKAAQQAYQQAQRGKVSLVSYLVQNKLVKSLVLAEIASEQFGVPFLDLSSLDKDSLPKGL
VSEKLIRQHNVLPLWRRGNKLFIGISDPTNHQAITDIQFNTGLTTEAILVEDNKLTDAIDRFFDSQSSGMGDLADVDLEG
LDVGLADDKKQDAIAGQDADDAPVVRFVNKMLLDAIKLGSSDLHFEPYEKSYRVRLRTDGILHEVAKPPIQLAGRIAARL
KVMAGLDISERRRPQDGRIKLRISKTKSIDFRVNTLPTLWGEKVVMRILDSSSAQMGIDALGYEPEQKALYLEALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVMSTLHTNSAAETLTRLHHMGVAGFNIATAINLIIAQRLARKLCPHCKKEIDIPRETLIKEGFPE
DRIGSFKIYAPVGCEHCNGGYKGRIGIYEVVKNTPALARIIMEEGNSMDIAIQMRKDGFNDLRTSGLMKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=876773 RHM55_RS11000 WP_322181950.1 2356850..2358550(+) (pilB) [Pseudomonas sp. MH9.2]
ATGACTGATGTTGTCCTTACCGGTTTGGCCAAGCAATTAGTGCTGACCAACCTGCTAAACGAAAAAGCGGCGCAACAGGC
GTATCAACAAGCCCAACGTGGCAAGGTTTCGCTGGTCAGCTACCTGGTGCAAAACAAGCTGGTGAAAAGCCTGGTGCTTG
CCGAGATCGCTTCCGAGCAATTTGGGGTGCCGTTCCTGGACCTGAGCAGCCTGGACAAAGACAGCCTGCCAAAAGGCCTG
GTCAGCGAAAAGCTGATTCGGCAGCACAATGTCTTGCCGTTGTGGCGTCGCGGCAATAAATTGTTCATCGGGATATCAGA
CCCCACTAACCACCAGGCCATCACCGATATACAGTTCAATACCGGGCTGACCACCGAAGCCATTCTGGTGGAAGACAACA
AGCTCACCGACGCCATCGACAGATTTTTCGACAGCCAATCCTCCGGGATGGGTGATCTCGCCGATGTCGATCTCGAAGGA
TTGGACGTCGGGCTCGCTGACGATAAAAAGCAGGACGCGATCGCAGGACAAGATGCCGACGACGCGCCAGTGGTGCGCTT
CGTCAATAAGATGCTGCTGGACGCCATCAAGCTGGGCTCATCGGACTTGCACTTCGAACCTTACGAAAAGTCTTACCGGG
TCAGGCTGCGCACCGACGGCATTTTGCATGAAGTCGCCAAACCACCGATCCAGCTGGCCGGCCGAATCGCGGCGCGCTTG
AAGGTGATGGCCGGGCTGGATATTTCCGAGCGGCGCAGGCCGCAGGATGGCCGGATCAAGCTGCGTATCTCAAAAACCAA
ATCGATCGATTTTCGGGTCAACACCCTGCCGACCCTCTGGGGCGAAAAGGTGGTGATGCGGATCCTCGACTCGTCCAGCG
CGCAAATGGGCATTGATGCCCTAGGCTATGAGCCCGAGCAAAAAGCGCTGTACCTGGAGGCGCTGAAACAACCGCAAGGG
ATGATTCTGGTCACCGGCCCCACGGGCTCGGGTAAAACCGTTTCGCTGTACACTGGCCTGAACATTCTCAATACCGTCGA
CATCAACATTTCAACCGCGGAAGACCCGGTAGAAATCAACCTGGAAGGCATAAACCAGGTCAACGTCAACCCAAAACAAG
GAATGGATTTCGCCCAAGCGCTGCGTGCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACAGCCGAAATTGCGATCAAGGCTTCGCAAACCGGGCACATGGTCATGTCCACGCTGCACACTAACAGTGCGGCGGA
AACCCTGACCCGCCTGCATCATATGGGCGTCGCGGGGTTTAATATAGCGACCGCGATCAACTTGATCATCGCTCAACGTC
TGGCACGCAAGCTCTGCCCCCACTGCAAAAAAGAAATTGATATTCCGCGTGAAACTCTGATCAAGGAGGGGTTCCCAGAG
GACCGTATCGGCAGTTTCAAGATTTATGCACCGGTCGGTTGCGAACACTGCAATGGCGGTTACAAAGGCCGTATCGGGAT
TTACGAAGTGGTGAAAAACACCCCTGCCCTGGCACGGATCATCATGGAGGAAGGCAACTCCATGGATATCGCGATCCAGA
TGCGTAAAGATGGCTTTAACGACCTACGCACCTCCGGCCTGATGAAGGCCATGCAGGGCATCACCAGCCTTGAAGAAATC
AACCGCGTGACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Acinetobacter baylyi ADP1

58.688

99.647

0.585
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

51.332

99.47

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

51.63

97.527

0.504
  pilB Vibrio campbellii strain DS40M4

49.735

99.823

0.496
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilF Thermus thermophilus HB27

38.462

98.763

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.306

95.936

0.367