Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RHM59_RS14670 Genome accession   NZ_CP133783
Coordinates   3316037..3318124 (-) Length   695 a.a.
NCBI ID   WP_322224523.1    Uniprot ID   A0AAU0TPL2
Organism   Pseudomonas sp. MH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3311037..3323124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM59_RS14655 (RHM59_14655) - 3312626..3314239 (-) 1614 WP_322224521.1 AAA family ATPase -
  RHM59_RS14660 (RHM59_14660) aroB 3314250..3315356 (-) 1107 WP_322167248.1 3-dehydroquinate synthase -
  RHM59_RS14665 (RHM59_14665) aroK 3315514..3316032 (-) 519 WP_322167247.1 shikimate kinase AroK -
  RHM59_RS14670 (RHM59_14670) pilQ 3316037..3318124 (-) 2088 WP_322224523.1 type IV pilus secretin PilQ Machinery gene
  RHM59_RS14675 (RHM59_14675) pilP 3318180..3318707 (-) 528 WP_322184269.1 type 4a pilus biogenesis lipoprotein PilP -
  RHM59_RS14680 (RHM59_14680) pilO 3318704..3319327 (-) 624 WP_322167244.1 type 4a pilus biogenesis protein PilO -
  RHM59_RS14685 (RHM59_14685) - 3319324..3319890 (-) 567 WP_322167243.1 PilN domain-containing protein -
  RHM59_RS14690 (RHM59_14690) comM 3319890..3320954 (-) 1065 WP_322167242.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74651.02 Da        Isoelectric Point: 5.1851

>NTDB_id=876745 RHM59_RS14670 WP_322224523.1 3316037..3318124(-) (pilQ) [Pseudomonas sp. MH10]
MNRIFSVIGVSLWVAMISPMLEAASLKTLDVAALPGDRVELKLTFDGPIPVPRGYTTEQPARIALDLPGVTSQLASKTRE
LGVGNARSVVVVEAKDRTRVIINMTTLAPYSSRVDGNNLFLVVGQGAATAQRSQPSTATGAARVSVPAAAPAKSYAAVGK
AIKNIDFQRGELGEGNVVIELTDSGIAPDIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVTASATGDKASINIE
PTGAFDYSAYQTDNKLTISVRPLSNDDVDRRNLDKLVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESMKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKTEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKALGVQWGGSTNASGNGKTT
VGGSGLVTTPTGTTPNLNSPYVDLGAANATSGIGISFLTNNTLLDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVADGETIVIGGV
FSNTQSKTVEKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=876745 RHM59_RS14670 WP_322224523.1 3316037..3318124(-) (pilQ) [Pseudomonas sp. MH10]
ATGAACAGGATTTTCTCGGTTATCGGCGTTTCGCTTTGGGTCGCGATGATATCGCCGATGCTCGAAGCTGCCAGCTTGAA
AACGCTGGATGTTGCGGCGTTGCCGGGAGACCGCGTAGAGCTGAAGTTGACCTTCGACGGGCCGATCCCCGTTCCGCGTG
GCTATACCACCGAGCAACCGGCGCGGATTGCGTTGGACTTGCCTGGCGTGACCAGTCAGTTGGCGAGCAAGACTCGCGAG
TTGGGCGTAGGGAATGCCCGCAGCGTGGTAGTGGTCGAAGCTAAAGACCGTACGCGGGTGATCATTAACATGACCACGCT
TGCGCCTTATAGCTCGCGGGTCGATGGCAACAACCTGTTCTTGGTGGTGGGTCAGGGCGCCGCGACGGCTCAACGCTCCC
AGCCAAGTACTGCCACGGGTGCAGCGCGGGTTTCGGTACCTGCCGCTGCTCCGGCTAAATCCTATGCCGCAGTGGGTAAG
GCGATCAAGAACATCGACTTCCAGCGCGGCGAGTTGGGCGAGGGTAACGTAGTCATCGAGTTGACCGACTCGGGCATCGC
TCCAGACATCCAGGAGCAGGGCGGTAAAATTCGCGTGGATTTTGCCAAGACCCAATTGCCGGACCCTTTGCGCGTTCGGC
TGGATGTGAAAGATTTCGCAACACCAGTGCAGTTCGTCACCGCCAGCGCGACGGGTGACAAAGCCAGTATCAATATTGAG
CCAACCGGGGCGTTCGATTACTCGGCCTACCAGACCGATAACAAGCTGACCATCAGCGTGCGTCCGTTGAGCAACGATGA
TGTGGATCGTCGCAACCTCGACAAGCTGGTCTACACCGGCGAAAAGCTTTCGCTGAACTTCCAGGACATCGACGTGCGCT
CGGTCCTGCAGTTGATTGCCGATTTTACCAACCTCAACCTAGTGGCCAGCGACACGGTGCAGGGCGGGATTACCTTGCGT
TTGCAGAACGTGCCGTGGGATCAGGCGCTGGATTTGGTGCTGAAAACCAAAGGCCTGGATAAGCGCAAAGTGGGCAACGT
ATTGCTAGTCGCGCCGGCAGACGAAATCGCTGCCCGTGAGCGGCAGGAGCTGGAGTCGATGAAGCAAATTGCCGAGCTGG
CACCGCTACGCCGCGAGCTGCTGCAAGTGAACTACGCCAAAGCCGCTGATATTGCCAAGCTATTCCAGTCAGTCACCAGC
GCCGAAGCTAAAACCGAAGAGCGCGGCTCGATTACCGTGGATGATCGGACTAACAACATTATTGCCTACCAGACCCAGGA
TCGACTCGATGAGTTGCGCCGAATTGTCTCGCAGTTGGACATCCCGGTACGTCAGGTGATGATCGAAGCGCGGATCGTCG
AAGCCAACGTCGGTTACGACAAGGCGCTGGGCGTGCAATGGGGCGGTTCGACCAATGCTAGCGGAAACGGCAAAACGACA
GTGGGCGGCTCCGGTCTGGTGACGACGCCCACGGGCACTACTCCAAACCTCAATTCTCCGTATGTCGATCTGGGCGCAGC
CAATGCGACCTCCGGAATCGGTATTTCCTTCCTGACCAACAATACGTTGCTGGACCTTCAGCTCAGCGCCATGGAGAAGA
CCGGAAACGGCGAAGTAGTTTCCCAGCCGAAAGTTGTCACCTCTGACAAGGAAACGGCGAAAATCCTGAAAGGTACGGAA
GTCCCGTATCAGGAGGCCAGCTCCAGCGGTGCAACCTCCGTATCCTTTAAAGAGGCTGCGTTGTCTTTGGAGGTAACCCC
GCAGATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTGACCAAGGATGAGCCGGACTACGTCAATACCGTGCTCG
GCGTACCGCCGATCAAGAAAAACGAAGTGAATGCCAAGGTGCTCGTAGCGGACGGTGAAACCATCGTGATTGGTGGCGTA
TTCTCCAACACCCAAAGTAAAACCGTTGAAAAGGTGCCATTTTTGGGTGATGTGCCGTATGTTGGCCGCCTTTTCCGGCG
CGACGTAGTCTCGGAAGCGAAATCCGAACTGCTGGTGTTTCTTACCCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(530-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.574

100

0.761
  comQ Acinetobacter baylyi ADP1

43.064

99.568

0.429
  pilQ Acinetobacter baumannii strain A118

38.74

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.74

100

0.416
  pilQ Legionella pneumophila strain ERS1305867

40.118

97.554

0.391
  pilQ Legionella pneumophila strain Lp02

39.823

97.554

0.388