Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   RHM59_RS14690 Genome accession   NZ_CP133783
Coordinates   3319890..3320954 (-) Length   354 a.a.
NCBI ID   WP_322167242.1    Uniprot ID   -
Organism   Pseudomonas sp. MH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3314890..3325954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM59_RS14665 (RHM59_14665) aroK 3315514..3316032 (-) 519 WP_322167247.1 shikimate kinase AroK -
  RHM59_RS14670 (RHM59_14670) pilQ 3316037..3318124 (-) 2088 WP_322224523.1 type IV pilus secretin PilQ Machinery gene
  RHM59_RS14675 (RHM59_14675) pilP 3318180..3318707 (-) 528 WP_322184269.1 type 4a pilus biogenesis lipoprotein PilP -
  RHM59_RS14680 (RHM59_14680) pilO 3318704..3319327 (-) 624 WP_322167244.1 type 4a pilus biogenesis protein PilO -
  RHM59_RS14685 (RHM59_14685) - 3319324..3319890 (-) 567 WP_322167243.1 PilN domain-containing protein -
  RHM59_RS14690 (RHM59_14690) comM 3319890..3320954 (-) 1065 WP_322167242.1 pilus assembly protein PilM Machinery gene
  RHM59_RS14695 (RHM59_14695) - 3321166..3323610 (+) 2445 WP_416194780.1 penicillin-binding protein 1A -
  RHM59_RS14700 (RHM59_14700) - 3323754..3325022 (-) 1269 WP_322167240.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38093.59 Da        Isoelectric Point: 4.6305

>NTDB_id=876746 RHM59_RS14690 WP_322167242.1 3319890..3320954(-) (comM) [Pseudomonas sp. MH10]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLIKAKTNVKNVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLTAQLGHDHEQLTVAVVDIGATMTTLSVLHSGRIIYTREQLFGGRQLTEEIQRRYGLVV
EEAGLAKKQGGLPDDYVTEVLQPFKDAVVQQVSRSLQFFFAAGQYNSVDYIMLAGGTASIAGLDHLIQQRIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=876746 RHM59_RS14690 WP_322167242.1 3319890..3320954(-) (comM) [Pseudomonas sp. MH10]
GTGTTCGAACTCTTCGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTAGATCCGGCACCCGCTACAAAGTTGAATCGTATGCAGTAGAACCGCTGCCCGCCAACGCTGTAGTAGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGTGTGCTGATCAAGGCCAAGACTAATGTCAAAAATGTGGCGGTT
GCAGTGGCCGGTTCTGCGGTCATTACAAAAACCATCGAGATGGATGCTGGCCTGTCAGACGATGACATGGAAAACCAGCT
GAAGATCGAGGCTGATCAGTACATTCCCTATCCGCTGGAAGAAGTCGCTATCGACTTTGAAGTGCAGGGTTATTCGGCGC
GCAACCCCGAGCGCGTCGAGGTATTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAAGTCCGTGAAGCAGCCTTGGCGCTT
GCGGGCCTGACAGCCAAAGTGGTTGACGTAGAAGCGTACGCATTGGAGCGTTCCTTTGGCCTTCTTACCGCGCAGCTTGG
CCATGATCACGAGCAACTGACCGTGGCAGTGGTCGATATCGGCGCCACCATGACCACCTTGAGCGTTCTGCACAGCGGTC
GAATTATCTATACCCGCGAGCAACTGTTCGGTGGCCGCCAACTCACTGAAGAAATTCAACGACGCTACGGCCTCGTAGTG
GAGGAGGCTGGCCTGGCGAAGAAGCAAGGCGGGCTGCCCGACGACTACGTGACCGAAGTGCTGCAGCCGTTCAAGGATGC
GGTGGTTCAACAAGTGTCGCGTTCGTTGCAGTTCTTCTTTGCTGCTGGCCAATACAACAGCGTGGATTACATCATGCTGG
CAGGCGGTACCGCCTCGATTGCGGGTCTGGATCATTTGATTCAACAACGCATCGGCACGCCAACACTGGTCGCCAACCCC
TTCGCTGATATGGCGCTGAGCAGCAAAGTCAATGCCGGGGCCTTGGCCAGTGATGCACCGGCATTGATGATTGCCTGTGG
TCTCGCGCTGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.09

100

0.534
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469