Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RHM68_RS01520 Genome accession   NZ_CP133782
Coordinates   326784..328871 (+) Length   695 a.a.
NCBI ID   WP_322220195.1    Uniprot ID   -
Organism   Pseudomonas sp. DC1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 321784..333871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM68_RS01500 (RHM68_01500) comM 323995..325059 (+) 1065 WP_322220191.1 pilus assembly protein PilM Machinery gene
  RHM68_RS01505 (RHM68_01505) - 325060..325626 (+) 567 WP_322220192.1 PilN domain-containing protein -
  RHM68_RS01510 (RHM68_01510) pilO 325623..326246 (+) 624 WP_322220193.1 type 4a pilus biogenesis protein PilO -
  RHM68_RS01515 (RHM68_01515) - 326243..326770 (+) 528 WP_322220194.1 pilus assembly protein PilP -
  RHM68_RS01520 (RHM68_01520) pilQ 326784..328871 (+) 2088 WP_322220195.1 type IV pilus secretin PilQ Machinery gene
  RHM68_RS01525 (RHM68_01525) aroK 328876..329394 (+) 519 WP_322220196.1 shikimate kinase AroK -
  RHM68_RS01530 (RHM68_01530) aroB 329545..330645 (+) 1101 WP_322220197.1 3-dehydroquinate synthase -
  RHM68_RS01535 (RHM68_01535) - 330656..332242 (+) 1587 WP_322220198.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75246.40 Da        Isoelectric Point: 5.8284

>NTDB_id=876708 RHM68_RS01520 WP_322220195.1 326784..328871(+) (pilQ) [Pseudomonas sp. DC1.2]
MNRIFSTVGISLWIALLSPMVQAANLKALDIATLPGDRVELKLSFDGAPPAPHGYTTESPARIALDLPGVVNKLANKNRE
LGGGNARSATVVEAKDRTRLIINLTQLTPYSTRVEGNNLFVVVGQGANSTASRAAASAPRTATAQAAPVKAYVPAGKAIR
GVDFQRGTQGEGNVVIDLSDPTIAPDIQEREGKIVLGFARTQLPDPLRVRLDVKDFATPVQFVNASATGDRATISIEPTG
AFEYSTYQTDNKLTVSIRPVTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSSES
KVDERGSITVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSLQNKGNWNTSGVS
NGTNASSTIGTPGSTSTNSPFVDMGTASNTSGIGISFITNNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVAFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=876708 RHM68_RS01520 WP_322220195.1 326784..328871(+) (pilQ) [Pseudomonas sp. DC1.2]
ATGAACAGGATTTTTTCAACCGTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTACAAGCGGCCAACCTCAA
GGCCCTGGATATCGCAACACTGCCAGGAGATCGTGTTGAGTTGAAATTGTCGTTTGATGGGGCGCCTCCTGCTCCTCACG
GCTACACCACCGAGTCTCCGGCGCGCATCGCACTGGATCTGCCAGGCGTGGTCAACAAGCTTGCGAACAAAAATCGCGAA
CTGGGTGGCGGCAATGCCCGCAGTGCTACGGTGGTGGAGGCCAAGGATCGCACGCGGCTGATCATCAACCTCACTCAACT
GACGCCCTACAGCACCAGAGTTGAAGGCAACAACCTGTTTGTAGTGGTCGGACAGGGGGCTAACAGCACCGCTTCCAGAG
CCGCCGCCAGCGCGCCGCGCACGGCCACCGCCCAAGCGGCGCCGGTGAAGGCCTATGTGCCAGCCGGCAAGGCCATCCGA
GGCGTGGATTTCCAGCGTGGCACGCAGGGTGAAGGCAATGTAGTGATCGACTTGTCGGACCCAACCATTGCGCCCGACAT
TCAGGAGCGCGAAGGCAAGATCGTTCTTGGGTTTGCCAGGACCCAATTGCCTGACCCGTTACGCGTGCGTCTGGACGTCA
AGGATTTCGCCACGCCAGTGCAGTTCGTTAACGCCAGTGCCACGGGTGACCGTGCCACGATCAGCATCGAGCCCACGGGT
GCCTTTGAATATTCGACCTACCAGACCGATAACAAGCTGACGGTCAGCATCCGCCCGGTGACTGTCGATGACCTGCAAAA
GCGCAACGCCGAACGTTTTGCTTACACCGGTGAAAAGCTTTCGCTGAACTTCCAGGACATCGATGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAACCTCAACCTGGTGGCCAGTGACACGGTGCAGGGCGGCATCACATTGCGTCTGCAGAAC
GTACCGTGGGATCAAGCGCTGGACTTGGTGCTGAAAACCAAAGGCCTGGACAAACGTAAGATCGGCAACGTGTTGCTGGT
AGCGCCAGCGGATGAAATAGCTGCCCGCGAGCGCCAGGAACTGGAGTCGCAAAAACAGATTGCCGAACTGGCACCCCTGC
GTCGTGAACTGTTGCAGGTCAACTACGCCAAAGCTGCGGACATCGCCAAGCTGTTCCAGTCAGTGACCAGTTCAGAATCT
AAAGTCGACGAGCGCGGTTCAATCACCGTCGACGATCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAGCTTCGCCGTATCGTCGCGCAACTGGACATTCCGGTGCGCCAAGTGATGATCGAGGCGCGGATCGTTGAGGCCAACG
TCGATTACGACAAGAGCCTGGGGGTTCGCTGGGGTGGCTCGCTTCAGAACAAGGGCAACTGGAACACATCCGGCGTCAGT
AACGGCACCAACGCTTCCTCGACCATAGGCACGCCTGGCAGCACCAGTACCAACTCGCCGTTTGTCGACATGGGCACCGC
CTCTAATACCTCGGGGATCGGCATTTCCTTCATCACTAACAATGTCCTGCTGGACCTTGAGCTGACAGCGATGGAGAAAA
CGGGCAACGGCGAAATCGTCTCGCAACCGAAGGTGGTCACGTCCGATAAGGAAACCGCAAAAATCCTCAAGGGCACCGAG
ATTCCGTATCAGGAAGCCAGCTCCAGCGGTGCGACGTCCGTCGCCTTCAAGGAAGCCTCGCTGTCCCTGGAGGTGACACC
GCAGATCACACCGGATAACCGCATCATCATGGAGGTCAAGGTCACGAAGGATGAGCCGGATTACCTGAACAAGGTGCAGG
ACGTTCCACCGATCAAGAAAAACGAAGTCAACGCCAAAGTGTTGGTCAACGACGGAGAGACCATCGTTATCGGTGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGACGTGGTTTCGGAGAAAAAATCCGAGCTACTGGTGTTTCTCACTCCACGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.302

100

0.765
  comQ Acinetobacter baylyi ADP1

42.407

100

0.426
  pilQ Acinetobacter baumannii strain A118

38.204

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.204

100

0.41
  pilQ Legionella pneumophila strain Lp02

37.316

97.554

0.364
  pilQ Legionella pneumophila strain ERS1305867

37.537

96.978

0.364