Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   RHM68_RS01500 Genome accession   NZ_CP133782
Coordinates   323995..325059 (+) Length   354 a.a.
NCBI ID   WP_322220191.1    Uniprot ID   -
Organism   Pseudomonas sp. DC1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318995..330059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM68_RS01485 (RHM68_01485) - 319313..319666 (+) 354 Protein_294 thermonuclease family protein -
  RHM68_RS01490 (RHM68_01490) - 319888..321156 (+) 1269 WP_322220189.1 malic enzyme-like NAD(P)-binding protein -
  RHM68_RS01495 (RHM68_01495) - 321335..323776 (-) 2442 WP_416195235.1 penicillin-binding protein 1A -
  RHM68_RS01500 (RHM68_01500) comM 323995..325059 (+) 1065 WP_322220191.1 pilus assembly protein PilM Machinery gene
  RHM68_RS01505 (RHM68_01505) - 325060..325626 (+) 567 WP_322220192.1 PilN domain-containing protein -
  RHM68_RS01510 (RHM68_01510) pilO 325623..326246 (+) 624 WP_322220193.1 type 4a pilus biogenesis protein PilO -
  RHM68_RS01515 (RHM68_01515) - 326243..326770 (+) 528 WP_322220194.1 pilus assembly protein PilP -
  RHM68_RS01520 (RHM68_01520) pilQ 326784..328871 (+) 2088 WP_322220195.1 type IV pilus secretin PilQ Machinery gene
  RHM68_RS01525 (RHM68_01525) aroK 328876..329394 (+) 519 WP_322220196.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38139.53 Da        Isoelectric Point: 4.7509

>NTDB_id=876707 RHM68_RS01500 WP_322220191.1 323995..325059(+) (comM) [Pseudomonas sp. DC1.2]
MLGLFNKKNGTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALARVLVKAKTSVRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLRVEADQYIPYPLDEVAIDFEVQDVSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVSGLDRLIEQRLGTRTQVANP
FADMSLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=876707 RHM68_RS01500 WP_322220191.1 323995..325059(+) (comM) [Pseudomonas sp. DC1.2]
GTGCTAGGACTCTTCAATAAAAAGAATGGGACGCTTCTGGGGATCGACATCAGCTCCACGTCTGTGAAGCTTTTGGAGCT
GAGCCGTCAGGGCGACCGTTACCGCGTCGAGGCTTATGCGGTCGAACCGCTGCCGGCCAGTGCGGTGGTCGAAAAAAACA
TCGCCGAACTCGAAGGCGTGGGTCAGGCGCTCGCGCGCGTCCTGGTCAAGGCCAAGACCAGCGTCAGGAACGTCGCCGTG
GCTGTGGCCGGTTCCGCGGTCATAACCAAGACGATCGAGATGGACGCCGGGCTGTCCGACGACGAGATGGAAAATCAGCT
CAGAGTTGAGGCCGACCAGTACATTCCCTATCCATTGGACGAGGTCGCCATCGACTTCGAGGTCCAGGACGTCTCTGCGC
GCAACCCTGAGCGAGTCAATGTGCTGCTGGCTGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCCCTTGCCCTG
GCCGGGCTGACGGCACGGGTGGTGGATGTCGAGGCTTACGCACTGGAGCGATCCTTTGGTTTGCTGGCCACCCAACTGGC
TGCTTCCCAAGAGCGCCTGACCGTTGCCGTGGTGGACATCGGTGCGACCATGACCACCTTGAGCGTGCTGCACAATGGCC
GGATCATCTACACCCGCGAGCAACTGTTCGGCGGTCGTCAGCTCACCGAGGAAATCCAGCGCCGTTACGGCTTGACGGTC
GAGCAGGCCGGGCTTGCGAAAAAGCAGGGCGGTTTACCCGACGACTACGTCAGCGAAGTGTTGCAGCCATTCCGTGAAGC
CTTGGTGCAGCAGGTATCCCGTTCGTTGCAGTTTTTCTTTGCCTCAGGTCAATACAACGCGGTCGATCACATCCTGCTGG
CTGGGGGCACCGCCTCCGTCTCCGGGCTTGATCGGCTGATCGAGCAGCGTTTGGGGACGCGTACCCAGGTGGCTAACCCT
TTTGCTGACATGTCCCTGAGCAGTAAGGTTAACGCGGGTGCTCTGGCCAGCGATGCGCCGGCATTGATGATTGCCTGCGG
GCTCGCTCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.627

100

0.576
  pilM Acinetobacter baumannii D1279779

57.345

100

0.573
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483