Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RHM64_RS01670 Genome accession   NZ_CP133776
Coordinates   356929..359016 (+) Length   695 a.a.
NCBI ID   WP_322167246.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 351929..364016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS01650 (RHM64_01650) comM 354099..355163 (+) 1065 WP_322167242.1 pilus assembly protein PilM Machinery gene
  RHM64_RS01655 (RHM64_01655) - 355163..355729 (+) 567 WP_322167243.1 PilN domain-containing protein -
  RHM64_RS01660 (RHM64_01660) pilO 355726..356349 (+) 624 WP_322167244.1 type 4a pilus biogenesis protein PilO -
  RHM64_RS01665 (RHM64_01665) pilP 356346..356873 (+) 528 WP_322167245.1 type 4a pilus biogenesis lipoprotein PilP -
  RHM64_RS01670 (RHM64_01670) pilQ 356929..359016 (+) 2088 WP_322167246.1 type IV pilus secretin PilQ Machinery gene
  RHM64_RS01675 (RHM64_01675) aroK 359021..359539 (+) 519 WP_322167247.1 shikimate kinase AroK -
  RHM64_RS01680 (RHM64_01680) aroB 359697..360803 (+) 1107 WP_322167248.1 3-dehydroquinate synthase -
  RHM64_RS01685 (RHM64_01685) - 360814..362427 (+) 1614 WP_322167249.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74665.05 Da        Isoelectric Point: 5.1851

>NTDB_id=876611 RHM64_RS01670 WP_322167246.1 356929..359016(+) (pilQ) [Pseudomonas sp. AH2]
MNRIFSVIGVSLWVAMISPMLEAASLKTLDVAALPGDRVELKLTFDGPIPVPRGYTTEQPARIALDLPGVTSQLASKTRE
LGVGNARSVVVVEAKDRTRVIINMTTLAPYSSRVDGNNLFLVVGQGAATAQRSQPSTATGAARVSVPAAAPAKSYAAVGK
AIKNIDFQRGELGEGNVVIELTDSGIAPDIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVTASATGDKASINIE
PTGAFDYSAYQTDNKLTISVRPLTNDDVDRRNLDKLVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESMKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKTEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKALGVQWGGSTNASGNGKTT
VGGSGLVTTPTGTTPNLNSPYVDLGAANATSGIGISFLTNNTLLDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGTE
VPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVADGETIVIGGV
FSNTQSKTVEKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=876611 RHM64_RS01670 WP_322167246.1 356929..359016(+) (pilQ) [Pseudomonas sp. AH2]
ATGAACAGGATTTTCTCGGTTATCGGCGTTTCGCTTTGGGTCGCGATGATATCGCCGATGCTCGAAGCTGCCAGCTTGAA
AACGCTGGATGTTGCGGCGTTGCCGGGAGACCGCGTAGAGCTGAAGTTGACCTTCGACGGGCCGATCCCCGTTCCGCGTG
GCTATACCACCGAGCAACCGGCGCGGATTGCGTTGGACTTGCCTGGCGTGACCAGTCAGTTGGCGAGCAAGACTCGCGAG
TTGGGCGTAGGGAATGCCCGCAGCGTGGTAGTGGTCGAAGCTAAAGACCGTACGCGGGTGATCATTAACATGACCACGCT
TGCGCCTTATAGCTCGCGGGTCGATGGCAACAACCTGTTCTTGGTGGTGGGTCAGGGCGCCGCGACGGCTCAACGCTCCC
AGCCAAGTACTGCCACGGGTGCAGCGCGGGTTTCGGTACCTGCCGCTGCTCCGGCTAAATCCTATGCCGCAGTGGGTAAG
GCGATCAAGAACATCGACTTCCAGCGCGGCGAGTTGGGCGAGGGTAACGTAGTCATCGAGTTGACCGACTCGGGCATCGC
TCCAGACATCCAGGAGCAGGGCGGTAAAATTCGCGTGGATTTTGCCAAGACCCAGTTGCCGGACCCTTTGCGCGTTCGGC
TGGATGTGAAAGATTTCGCAACACCAGTGCAGTTCGTCACTGCCAGCGCTACGGGTGACAAAGCCAGTATCAATATTGAG
CCAACCGGGGCGTTCGATTACTCGGCCTACCAGACCGATAACAAGCTGACCATCAGCGTGCGTCCGTTGACCAACGATGA
TGTGGATCGTCGCAACCTCGACAAGCTGGTCTACACCGGCGAAAAGCTTTCGCTGAACTTCCAGGACATCGACGTGCGCT
CGGTCCTGCAGTTGATTGCCGATTTTACCAATCTCAACCTAGTGGCCAGCGACACGGTGCAGGGCGGGATTACCTTGCGT
CTGCAGAACGTGCCGTGGGATCAGGCGCTGGATTTGGTGCTGAAAACCAAAGGCCTGGATAAGCGCAAAGTGGGCAACGT
ATTGCTGGTCGCGCCGGCAGACGAAATCGCTGCCCGTGAGCGGCAGGAGCTGGAGTCGATGAAGCAAATTGCCGAGCTGG
CACCGCTACGCCGCGAGCTGCTGCAAGTGAACTACGCCAAAGCCGCTGATATTGCCAAGCTATTCCAGTCAGTCACCAGC
GCCGAAGCTAAAACCGAAGAGCGCGGTTCGATTACCGTGGATGATCGGACTAACAACATCATTGCCTACCAGACCCAGGA
TCGACTCGATGAGTTGCGCCGAATTGTCTCGCAGTTGGACATCCCGGTACGTCAGGTGATGATCGAAGCGCGGATCGTCG
AAGCCAACGTCGGTTACGACAAGGCGCTGGGCGTGCAATGGGGCGGTTCGACCAATGCTAGCGGTAACGGCAAAACGACA
GTGGGCGGCTCCGGTCTGGTGACGACGCCCACTGGCACTACTCCAAACCTCAATTCTCCGTATGTCGATCTGGGCGCAGC
CAATGCGACCTCCGGAATCGGTATTTCCTTCCTGACCAACAATACGTTGCTGGACCTTCAGCTCAGCGCCATGGAGAAGA
CCGGAAACGGCGAAGTAGTTTCCCAGCCGAAAGTTGTCACCTCTGACAAGGAAACGGCGAAAATCCTGAAAGGTACGGAA
GTCCCGTATCAGGAGGCCAGCTCCAGCGGTGCAACCTCCGTATCCTTTAAAGAGGCTGCGTTGTCTTTGGAGGTAACCCC
GCAGATCACGCCGGATAACCGCATCATCATGGAGGTCAAGGTGACCAAGGATGAGCCGGACTACGTCAATACCGTGCTCG
GCGTACCGCCGATCAAGAAAAACGAAGTGAATGCCAAGGTGCTCGTAGCGGACGGTGAAACCATCGTGATTGGTGGCGTA
TTCTCCAACACCCAAAGTAAAACCGTTGAAAAGGTGCCATTTTTGGGTGATGTGCCGTATGTTGGCCGCCTTTTCCGGCG
CGACGTAGTCTCGGAAGCAAAATCCGAACTGCTGGTGTTTCTTACCCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.713

100

0.763
  comQ Acinetobacter baylyi ADP1

43.064

99.568

0.429
  pilQ Acinetobacter baumannii strain A118

38.74

100

0.416
  pilQ Acinetobacter baumannii D1279779

38.74

100

0.416
  pilQ Legionella pneumophila strain ERS1305867

40.265

97.554

0.393
  pilQ Legionella pneumophila strain Lp02

39.971

97.554

0.39