Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   RHM64_RS01650 Genome accession   NZ_CP133776
Coordinates   354099..355163 (+) Length   354 a.a.
NCBI ID   WP_322167242.1    Uniprot ID   -
Organism   Pseudomonas sp. AH2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349099..360163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM64_RS01640 (RHM64_01640) - 350031..351299 (+) 1269 WP_322167240.1 malic enzyme-like NAD(P)-binding protein -
  RHM64_RS01645 (RHM64_01645) - 351443..353887 (-) 2445 WP_416194780.1 penicillin-binding protein 1A -
  RHM64_RS01650 (RHM64_01650) comM 354099..355163 (+) 1065 WP_322167242.1 pilus assembly protein PilM Machinery gene
  RHM64_RS01655 (RHM64_01655) - 355163..355729 (+) 567 WP_322167243.1 PilN domain-containing protein -
  RHM64_RS01660 (RHM64_01660) pilO 355726..356349 (+) 624 WP_322167244.1 type 4a pilus biogenesis protein PilO -
  RHM64_RS01665 (RHM64_01665) pilP 356346..356873 (+) 528 WP_322167245.1 type 4a pilus biogenesis lipoprotein PilP -
  RHM64_RS01670 (RHM64_01670) pilQ 356929..359016 (+) 2088 WP_322167246.1 type IV pilus secretin PilQ Machinery gene
  RHM64_RS01675 (RHM64_01675) aroK 359021..359539 (+) 519 WP_322167247.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38093.59 Da        Isoelectric Point: 4.6305

>NTDB_id=876610 RHM64_RS01650 WP_322167242.1 354099..355163(+) (comM) [Pseudomonas sp. AH2]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLIKAKTNVKNVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLTAQLGHDHEQLTVAVVDIGATMTTLSVLHSGRIIYTREQLFGGRQLTEEIQRRYGLVV
EEAGLAKKQGGLPDDYVTEVLQPFKDAVVQQVSRSLQFFFAAGQYNSVDYIMLAGGTASIAGLDHLIQQRIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=876610 RHM64_RS01650 WP_322167242.1 354099..355163(+) (comM) [Pseudomonas sp. AH2]
GTGTTCGAACTCTTCGGTAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTAGATCCGGCACCCGCTACAAAGTTGAATCGTATGCAGTAGAACCGCTGCCCGCCAACGCTGTAGTAGAAAAGAATA
TTGCCGAGCTCGAAGGGGTTGGTCAGGCGCTGTCTCGTGTGCTGATCAAGGCCAAGACTAATGTCAAAAATGTGGCGGTT
GCAGTGGCCGGTTCTGCGGTCATTACCAAAACCATCGAGATGGATGCTGGCCTGTCAGACGATGACATGGAAAACCAGCT
GAAGATCGAGGCTGATCAGTACATTCCCTATCCGCTGGAAGAAGTCGCTATCGACTTTGAAGTGCAGGGTTATTCGGCGC
GCAACCCCGAGCGCGTCGAGGTATTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAAGTCCGTGAAGCAGCCTTGGCGCTT
GCGGGCCTGACAGCCAAAGTGGTTGACGTAGAAGCGTACGCATTGGAGCGTTCCTTTGGCCTTCTTACCGCGCAGCTTGG
CCATGATCACGAGCAACTGACCGTGGCAGTGGTCGATATCGGCGCCACCATGACCACCTTGAGCGTTCTGCACAGCGGTC
GAATTATCTATACCCGCGAGCAACTGTTCGGTGGCCGCCAACTCACTGAAGAAATTCAACGACGCTACGGCCTCGTAGTG
GAGGAGGCTGGCCTGGCGAAGAAGCAAGGCGGGCTGCCCGACGACTACGTGACCGAAGTGCTGCAGCCGTTCAAGGATGC
GGTGGTTCAACAAGTGTCGCGTTCGTTGCAGTTCTTCTTTGCTGCTGGCCAATACAACAGCGTGGATTACATCATGCTGG
CAGGCGGTACCGCCTCGATTGCGGGTCTGGATCATTTGATTCAACAACGCATCGGCACGCCAACACTGGTCGCCAACCCC
TTCGCTGATATGGCGCTGAGCAGCAAAGTCAACGCCGGGGCCTTGGCCAGTGATGCACCGGCATTGATGATTGCCTGTGG
TCTCGCGCTGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.09

100

0.534
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469