Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RG903_RS03405 Genome accession   NZ_CP133759
Coordinates   679640..681361 (-) Length   573 a.a.
NCBI ID   WP_028988546.1    Uniprot ID   -
Organism   Thermithiobacillus tepidarius DSM 3134     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 674640..686361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG903_RS03390 (RG903_03380) pilR 675295..676656 (-) 1362 WP_211218612.1 sigma-54-dependent transcriptional regulator Regulator
  RG903_RS03395 (RG903_03385) - 676653..678362 (-) 1710 WP_051180642.1 two-component system sensor histidine kinase NtrB -
  RG903_RS03400 (RG903_03390) pilC 678376..679623 (-) 1248 WP_051180641.1 type II secretion system F family protein Machinery gene
  RG903_RS03405 (RG903_03395) pilB 679640..681361 (-) 1722 WP_028988546.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RG903_RS03410 (RG903_03400) aroE 681328..682200 (-) 873 WP_028988545.1 shikimate dehydrogenase -
  RG903_RS03415 (RG903_03405) - 682393..683022 (+) 630 WP_028988544.1 sulfite oxidase-like oxidoreductase -
  RG903_RS03420 (RG903_03410) - 683038..683466 (+) 429 WP_028988543.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  RG903_RS03425 (RG903_03415) - 683480..685396 (-) 1917 WP_028988542.1 ATP-binding cassette domain-containing protein -
  RG903_RS03430 (RG903_03420) - 685409..686008 (-) 600 WP_028988541.1 histidine phosphatase family protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63190.77 Da        Isoelectric Point: 6.6647

>NTDB_id=876392 RG903_RS03405 WP_028988546.1 679640..681361(-) (pilB) [Thermithiobacillus tepidarius DSM 3134]
MASVNPNSKPLNSLAQALIQQGLCSEEVVARAVQDAQQQHTTLVALLVRNGVVDPKRLMRSLSERYGLSMLDLDAVALDD
EVSGLLDRKLIRRYHALPLSKRCKHLYLAVSDPTDFSAFDDIKFNTGLQVMPVVVEEDKLNRLIEGLLGQMEGNIADLIG
NGSFNATLESEEDAEVSLDQMASGGDDAPVVKFVQHLLLDSIQRGASDVHLEPYEKSFRVRYRIDGILQEIIQPPLGLRE
GITSRLKILARLDISERRIPQDGRLRVRLSKERAIDFRISFLPTLYGEKIVLRLLDPASARVGIDKLGFFPEQQAAFLEA
IHRPYGMVLVTGPTGSGKTVTLYTALNILNTPGVNISTAEDPVEINLHGINQVNVNDKIGMGFSTALRAFLRQDPDIIMV
GEIRDLETAEISVKAAQTGHMVLSTLHTNDAPQTLTRLENMGVPTFNIASAVQLVLAQRLARRLCEHCKKPTSIPEPAML
EAGFSKEQLKGWRPYTADGCEKCNKTGYKGRVGLYQVMPISEVMRNMILEGKTAADLARQAAAEGILTLRQSGLRRVMDG
TTSLQEVTRVTNL

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=876392 RG903_RS03405 WP_028988546.1 679640..681361(-) (pilB) [Thermithiobacillus tepidarius DSM 3134]
ATGGCTAGCGTCAACCCAAACAGCAAACCACTGAATTCGCTTGCGCAGGCGCTGATACAGCAGGGACTCTGCTCGGAGGA
GGTGGTCGCCCGCGCGGTGCAGGACGCCCAGCAGCAACACACCACCCTGGTGGCGCTGCTGGTGCGCAACGGCGTCGTCG
ATCCCAAGCGCCTGATGCGCAGCCTGAGCGAGCGCTACGGCCTCTCCATGCTGGATCTGGACGCCGTGGCGCTGGATGAC
GAAGTCAGCGGCCTGCTCGATCGCAAGCTGATCCGCCGCTACCACGCCCTGCCCCTGTCCAAACGCTGCAAGCACCTCTA
CCTGGCCGTCTCCGATCCCACCGACTTCAGCGCCTTCGACGACATCAAGTTCAATACGGGCCTGCAGGTCATGCCCGTGG
TGGTGGAGGAGGACAAGCTCAACCGCCTGATCGAAGGCCTGCTCGGCCAGATGGAAGGCAATATTGCCGATCTCATCGGC
AACGGCAGCTTCAACGCCACCCTCGAGAGCGAGGAGGACGCTGAGGTCAGCCTCGACCAGATGGCCTCGGGCGGCGACGA
CGCGCCGGTAGTCAAGTTCGTACAGCATCTGCTGCTGGACTCCATCCAGCGCGGCGCCTCCGACGTGCATCTGGAACCTT
ACGAAAAATCCTTCCGGGTGCGTTACCGCATCGACGGCATCCTGCAGGAAATCATCCAGCCGCCCCTGGGCCTGCGCGAA
GGCATCACCTCGCGTCTCAAGATCCTGGCGCGCTTGGACATCAGCGAGCGGCGCATTCCCCAGGACGGCCGCCTGCGGGT
GCGCCTGTCCAAGGAGCGGGCCATCGACTTCCGCATCAGCTTTCTGCCCACCCTCTACGGCGAGAAGATCGTGCTGCGCC
TGCTGGACCCGGCCAGCGCCCGGGTCGGCATCGACAAGCTCGGCTTCTTCCCGGAGCAGCAGGCAGCCTTCCTGGAAGCC
ATCCACCGGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGACTCTGTACACTGCGCTGAA
CATCCTGAATACCCCGGGCGTGAACATCAGCACCGCCGAGGACCCGGTGGAAATCAACCTGCACGGCATCAACCAGGTCA
ACGTCAACGACAAGATCGGCATGGGCTTTTCCACCGCCCTGCGCGCCTTCCTGCGTCAGGACCCCGACATCATCATGGTC
GGCGAAATCCGCGATCTGGAAACGGCGGAAATTTCGGTCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCACCCTGCA
CACCAACGATGCGCCGCAGACGCTGACCCGCCTGGAGAACATGGGCGTGCCCACCTTCAACATTGCCTCGGCGGTGCAAT
TGGTGCTGGCCCAGCGCCTGGCGCGGCGTCTGTGCGAGCATTGCAAGAAGCCAACCAGCATCCCCGAGCCCGCCATGCTG
GAGGCCGGGTTCAGCAAGGAGCAGCTGAAGGGCTGGCGGCCCTACACCGCCGACGGCTGCGAAAAGTGCAACAAGACCGG
CTACAAGGGCCGCGTCGGTCTTTATCAGGTCATGCCCATCTCGGAAGTCATGCGCAACATGATCCTGGAAGGCAAGACCG
CGGCGGATCTGGCACGGCAGGCGGCCGCCGAGGGTATCCTCACCCTGCGCCAATCGGGCCTGCGCCGGGTGATGGACGGC
ACCACCAGCTTGCAGGAAGTAACCCGCGTAACCAATCTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-152)

Type II/IV secretion system protein (T2SSE)

(197-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.035

98.604

0.513
  pilB Acinetobacter baylyi ADP1

51.318

99.302

0.51
  pilB Legionella pneumophila strain ERS1305867

50

98.429

0.492
  pilF Neisseria gonorrhoeae MS11

48.233

98.778

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

46.466

98.778

0.459
  pilB Vibrio cholerae strain A1552

48.968

93.019

0.455
  pilB Vibrio campbellii strain DS40M4

46.018

98.604

0.454
  pilF Thermus thermophilus HB27

41.404

99.476

0.412
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.459

98.08

0.407