Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RG903_RS03400 Genome accession   NZ_CP133759
Coordinates   678376..679623 (-) Length   415 a.a.
NCBI ID   WP_051180641.1    Uniprot ID   -
Organism   Thermithiobacillus tepidarius DSM 3134     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 673376..684623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG903_RS03380 (RG903_03370) coaE 673736..674398 (-) 663 WP_051180643.1 dephospho-CoA kinase -
  RG903_RS03385 (RG903_03375) - 674403..675206 (-) 804 WP_028988549.1 prepilin peptidase -
  RG903_RS03390 (RG903_03380) pilR 675295..676656 (-) 1362 WP_211218612.1 sigma-54-dependent transcriptional regulator Regulator
  RG903_RS03395 (RG903_03385) - 676653..678362 (-) 1710 WP_051180642.1 two-component system sensor histidine kinase NtrB -
  RG903_RS03400 (RG903_03390) pilC 678376..679623 (-) 1248 WP_051180641.1 type II secretion system F family protein Machinery gene
  RG903_RS03405 (RG903_03395) pilB 679640..681361 (-) 1722 WP_028988546.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RG903_RS03410 (RG903_03400) aroE 681328..682200 (-) 873 WP_028988545.1 shikimate dehydrogenase -
  RG903_RS03415 (RG903_03405) - 682393..683022 (+) 630 WP_028988544.1 sulfite oxidase-like oxidoreductase -
  RG903_RS03420 (RG903_03410) - 683038..683466 (+) 429 WP_028988543.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 45022.43 Da        Isoelectric Point: 10.0847

>NTDB_id=876391 RG903_RS03400 WP_051180641.1 678376..679623(-) (pilC) [Thermithiobacillus tepidarius DSM 3134]
MAQAAQEAAVRSKTVADLEFVWEGKDTLGAIKKGEIRAPSEAYARAMLRRQGILPTRVKKKQKSLLGDRSIKEKDLVVFT
RQLATMLNAGLPIVQAIELIGAGASNKGMQKLLKSIQTDVESGERLSSALAKFPAYFDKLYVALVSAGELGGILDTILLK
LATYREKSYALKAKVKSALFYPTSIIVVAFIITAILMIFVIPKFAEMFSGFGAELPALTSFVIKLSNGFVRYWYLVFGIP
IAGLVGLRLAYKRVPKVHAAFDRMLLQMPVLGDILLKAAIARFTRTFSTMHAAGVPMTESLETIARTSGNTVIEDAIMKA
REAVAAGQRLSQPLAEAGIFPPMVVQMINIGEESGALETMLAKVADFYESEVDEAVNRMTSLMEPMIMAFLGVVIGGLVI
AMYLPIFKLASVVGG

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=876391 RG903_RS03400 WP_051180641.1 678376..679623(-) (pilC) [Thermithiobacillus tepidarius DSM 3134]
ATGGCGCAAGCGGCACAGGAAGCGGCAGTCAGGAGCAAAACCGTAGCGGACCTGGAATTCGTGTGGGAGGGCAAGGACAC
GCTGGGGGCGATCAAGAAAGGCGAGATCCGTGCCCCATCGGAGGCTTACGCGCGGGCCATGCTGCGCCGACAGGGCATCT
TGCCGACCCGGGTGAAGAAGAAGCAGAAATCCCTGCTCGGCGACCGCAGCATCAAGGAAAAGGATCTGGTGGTCTTCACC
CGTCAGCTCGCCACCATGCTCAACGCCGGCTTGCCCATTGTGCAGGCGATCGAGCTGATCGGCGCGGGCGCCAGCAACAA
GGGCATGCAGAAGCTGCTCAAAAGCATCCAGACGGATGTCGAGTCGGGCGAGCGCCTGTCCAGCGCCCTGGCCAAGTTCC
CCGCCTACTTCGACAAGCTGTACGTGGCGCTGGTCTCCGCCGGCGAGCTGGGCGGCATCCTGGATACCATTCTGCTCAAG
CTGGCCACCTACCGGGAAAAGAGCTACGCCCTGAAGGCAAAAGTCAAATCGGCCCTCTTCTATCCTACCAGCATCATCGT
GGTCGCCTTCATCATCACCGCCATCCTGATGATCTTCGTGATTCCGAAATTCGCTGAAATGTTCTCCGGCTTCGGCGCCG
AGCTGCCGGCGCTGACCTCCTTCGTCATCAAGCTGTCCAACGGCTTCGTCCGCTACTGGTACCTGGTCTTCGGCATTCCC
ATCGCCGGTCTCGTCGGCCTGCGCCTGGCCTACAAGCGGGTGCCCAAGGTCCATGCCGCCTTCGACCGCATGCTGCTGCA
GATGCCGGTGCTGGGCGACATCCTGCTCAAGGCGGCCATCGCCCGCTTCACCCGCACCTTCTCCACCATGCACGCGGCGG
GCGTGCCCATGACCGAGTCCCTGGAGACCATTGCCCGCACCTCGGGCAATACGGTGATCGAAGACGCGATCATGAAGGCG
CGCGAGGCCGTGGCGGCCGGCCAGCGCCTGTCTCAGCCCCTGGCCGAGGCCGGCATCTTCCCGCCCATGGTCGTGCAGAT
GATCAACATCGGCGAGGAGTCCGGCGCCCTGGAGACCATGCTCGCCAAGGTGGCGGATTTCTACGAAAGCGAGGTGGATG
AGGCGGTGAACCGCATGACCAGCCTGATGGAACCCATGATCATGGCCTTCCTGGGCGTGGTCATCGGCGGCCTCGTGATC
GCCATGTATCTGCCCATCTTCAAGCTCGCCTCCGTCGTGGGTGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-202)

Type II secretion system (T2SS), protein F (T2SSF)

(283-405)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

49.261

97.831

0.482
  pilC Legionella pneumophila strain ERS1305867

47.619

96.145

0.458
  pilC Acinetobacter baylyi ADP1

45.592

95.663

0.436
  pilC Acinetobacter baumannii D1279779

45.34

95.663

0.434
  pilG Neisseria gonorrhoeae MS11

44.389

96.627

0.429
  pilG Neisseria meningitidis 44/76-A

44.361

96.145

0.427
  pilC Vibrio cholerae strain A1552

43.457

97.59

0.424
  pilC Vibrio campbellii strain DS40M4

40.602

96.145

0.39
  pilC Thermus thermophilus HB27

38.442

95.904

0.369