Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpP   Type   Regulator
Locus tag   RFN65_RS03445 Genome accession   NZ_CP133703
Coordinates   685017..685610 (-) Length   197 a.a.
NCBI ID   WP_003228214.1    Uniprot ID   A0A199WEF2
Organism   Bacillus subtilis strain CP35     
Function   degradation of ComK; degradation of DegU (predicted from homology)   
Competence regulation

Genomic Context

Location: 680017..690610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS03430 (RFN65_03430) mdxK 680332..682605 (+) 2274 WP_309530305.1 maltose phosphorylase -
  RFN65_RS03435 (RFN65_03435) malL 682598..684283 (+) 1686 WP_309530059.1 oligo-1,6-glucosidase -
  RFN65_RS03440 (RFN65_03440) pgmB 684280..684954 (+) 675 WP_181220172.1 beta-phosphoglucomutase -
  RFN65_RS03445 (RFN65_03445) clpP 685017..685610 (-) 594 WP_003228214.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP Regulator
  RFN65_RS03455 (RFN65_03455) yvdQ 686111..686623 (+) 513 WP_015384719.1 DUF3231 family protein -
  RFN65_RS03460 (RFN65_03460) psmB 686659..686979 (-) 321 WP_021480661.1 QacE family quaternary ammonium compound efflux SMR transporter -
  RFN65_RS03465 (RFN65_03465) psmA 686979..687314 (-) 336 WP_063334703.1 SMR family transporter -
  RFN65_RS03470 (RFN65_03470) - 687396..687980 (-) 585 WP_309530060.1 TetR family transcriptional regulator -
  RFN65_RS03475 (RFN65_03475) aspP 688074..689636 (-) 1563 WP_015384716.1 aspartate/proton symporter AspP -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21682.00 Da        Isoelectric Point: 4.9416

>NTDB_id=875808 RFN65_RS03445 WP_003228214.1 685017..685610(-) (clpP) [Bacillus subtilis strain CP35]
MNLIPTVIEQTNRGERAYDIYSRLLKDRIIMLGSAIDDNVANSIVSQLLFLAAEDPEKEISLYINSPGGSITAGMAIYDT
MQFIKPKVSTICIGMAASMGAFLLAAGEKGKRYALPNSEVMIHQPLGGAQGQATEIEIAAKRILLLRDKLNKVLAERTGQ
PLEVIERDTDRDNFKSAEEALEYGLIDKILTHTEDKK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=875808 RFN65_RS03445 WP_003228214.1 685017..685610(-) (clpP) [Bacillus subtilis strain CP35]
ATGAATTTAATACCTACAGTCATTGAACAAACGAACCGCGGGGAAAGAGCGTATGACATTTATTCTCGTCTATTAAAGGA
TCGTATCATCATGCTTGGATCTGCGATTGATGACAACGTTGCGAACTCCATCGTGTCACAGCTTTTATTCTTAGCAGCAG
AAGACCCTGAAAAAGAAATTTCTCTTTACATCAACAGCCCGGGCGGCTCTATTACAGCTGGTATGGCGATCTATGATACC
ATGCAGTTTATTAAGCCGAAGGTATCTACAATTTGTATCGGTATGGCTGCGTCAATGGGCGCGTTCCTGCTTGCAGCCGG
CGAAAAAGGCAAACGCTATGCGCTTCCAAACAGTGAAGTCATGATTCACCAGCCACTTGGCGGTGCGCAAGGTCAGGCGA
CAGAAATTGAAATTGCCGCGAAACGCATTCTCTTGCTTCGCGACAAATTAAACAAAGTCCTTGCTGAACGTACTGGCCAG
CCGCTTGAAGTGATCGAACGCGACACAGACCGTGATAACTTCAAGTCTGCTGAAGAAGCGCTTGAATACGGCCTGATTGA
CAAAATTTTGACTCACACAGAAGACAAAAAGTAA

Domains

Predicted by InterproScan.

Clp protease (CLP_protease)

(13-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WEF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpP Bacillus subtilis subsp. subtilis str. 168

100

100

1
  clpP Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

68.617

95.431

0.655
  clpP Streptococcus thermophilus LMG 18311

58.163

99.492

0.579
  clpP Streptococcus thermophilus LMD-9

58.163

99.492

0.579
  clpP Streptococcus pneumoniae D39

57.812

97.462

0.563
  clpP Streptococcus pneumoniae Rx1

57.812

97.462

0.563
  clpP Streptococcus pneumoniae R6

57.812

97.462

0.563
  clpP Streptococcus pneumoniae TIGR4

57.812

97.462

0.563
  clpP Lactococcus lactis subsp. cremoris KW2

57.895

96.447

0.558
  clpP Streptococcus pyogenes JRS4

56.122

99.492

0.558
  clpP Streptococcus pyogenes MGAS315

56.122

99.492

0.558
  clpP Streptococcus mutans UA159

54.822

100

0.548
  clpP Lactococcus lactis subsp. lactis strain DGCC12653

55.789

96.447

0.538