Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RFF20_RS05735 Genome accession   NZ_CP133639
Coordinates   1223357..1224742 (-) Length   461 a.a.
NCBI ID   WP_324005464.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1218357..1229742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF20_RS05705 (RFF20_05705) - 1218484..1219857 (-) 1374 WP_096743226.1 alanine/glycine:cation symporter family protein -
  RFF20_RS05710 (RFF20_05710) - 1220306..1220575 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF20_RS05715 (RFF20_05715) yacG 1220575..1220778 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  RFF20_RS05720 (RFF20_05720) coaE 1220789..1221409 (-) 621 WP_115097849.1 dephospho-CoA kinase -
  RFF20_RS05725 (RFF20_05725) - 1221421..1222146 (-) 726 WP_324005461.1 A24 family peptidase -
  RFF20_RS05730 (RFF20_05730) pilC 1222146..1223357 (-) 1212 WP_324005463.1 type II secretion system F family protein Machinery gene
  RFF20_RS05735 (RFF20_05735) pilB 1223357..1224742 (-) 1386 WP_324005464.1 GspE/PulE family protein Machinery gene
  RFF20_RS05740 (RFF20_05740) pilA 1224742..1225176 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  RFF20_RS05745 (RFF20_05745) ampD 1225336..1225902 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF20_RS05750 (RFF20_05750) rppH 1226488..1227084 (+) 597 WP_324005467.1 RNA pyrophosphohydrolase -
  RFF20_RS05755 (RFF20_05755) - 1227087..1227881 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  RFF20_RS05760 (RFF20_05760) lgt 1227891..1228694 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF20_RS05765 (RFF20_05765) - 1228700..1229551 (+) 852 WP_324005469.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52035.61 Da        Isoelectric Point: 6.5002

>NTDB_id=875418 RFF20_RS05735 WP_324005464.1 1223357..1224742(-) (pilB) [Pasteurella multocida strain Past6]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
KNELKRALQQLSPPELQVEESIPTAYQVVQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYTALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGAYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=875418 RFF20_RS05735 WP_324005464.1 1223357..1224742(-) (pilB) [Pasteurella multocida strain Past6]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAGCCTGTTTTATTATCA
AAAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAGAATTACAAGTAGAAGAAAGCATACCCACGGCTTATCA
AGTCGTGCAATTAGATTCTGATTCCGCAACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGATATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCAGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACACTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TACCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGCATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

57.918

100

0.579
  pilB Acinetobacter baumannii D1279779

40

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.333

100

0.421
  pilB Vibrio campbellii strain DS40M4

40.249

100

0.421
  pilB Acinetobacter baylyi ADP1

38.477

100

0.416
  pilB Vibrio cholerae strain A1552

39.3

100

0.414
  pilB Legionella pneumophila strain ERS1305867

37.014

100

0.393