Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RFF20_RS05740 Genome accession   NZ_CP133639
Coordinates   1224742..1225176 (-) Length   144 a.a.
NCBI ID   WP_083004804.1    Uniprot ID   A0A1Q1PRS7
Organism   Pasteurella multocida strain Past6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1219742..1230176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF20_RS05710 (RFF20_05710) - 1220306..1220575 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF20_RS05715 (RFF20_05715) yacG 1220575..1220778 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  RFF20_RS05720 (RFF20_05720) coaE 1220789..1221409 (-) 621 WP_115097849.1 dephospho-CoA kinase -
  RFF20_RS05725 (RFF20_05725) - 1221421..1222146 (-) 726 WP_324005461.1 A24 family peptidase -
  RFF20_RS05730 (RFF20_05730) pilC 1222146..1223357 (-) 1212 WP_324005463.1 type II secretion system F family protein Machinery gene
  RFF20_RS05735 (RFF20_05735) pilB 1223357..1224742 (-) 1386 WP_324005464.1 GspE/PulE family protein Machinery gene
  RFF20_RS05740 (RFF20_05740) pilA 1224742..1225176 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  RFF20_RS05745 (RFF20_05745) ampD 1225336..1225902 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF20_RS05750 (RFF20_05750) rppH 1226488..1227084 (+) 597 WP_324005467.1 RNA pyrophosphohydrolase -
  RFF20_RS05755 (RFF20_05755) - 1227087..1227881 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  RFF20_RS05760 (RFF20_05760) lgt 1227891..1228694 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF20_RS05765 (RFF20_05765) - 1228700..1229551 (+) 852 WP_324005469.1 thymidylate synthase -
  RFF20_RS05770 (RFF20_05770) tadA 1229578..1230066 (+) 489 WP_324005471.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.46 Da        Isoelectric Point: 8.8040

>NTDB_id=875419 RFF20_RS05740 WP_083004804.1 1224742..1225176(-) (pilA) [Pasteurella multocida strain Past6]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=875419 RFF20_RS05740 WP_083004804.1 1224742..1225176(-) (pilA) [Pasteurella multocida strain Past6]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q1PRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

34.437

100

0.361