Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RFF38_RS06865 Genome accession   NZ_CP133638
Coordinates   1420721..1422109 (-) Length   462 a.a.
NCBI ID   WP_115097850.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1415721..1427109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF38_RS06835 (RFF38_06835) - 1415848..1417221 (-) 1374 WP_096743226.1 alanine/glycine:cation symporter family protein -
  RFF38_RS06840 (RFF38_06840) - 1417670..1417939 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF38_RS06845 (RFF38_06845) yacG 1417939..1418142 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  RFF38_RS06850 (RFF38_06850) coaE 1418153..1418773 (-) 621 WP_096743227.1 dephospho-CoA kinase -
  RFF38_RS06855 (RFF38_06855) - 1418785..1419510 (-) 726 WP_096743228.1 prepilin peptidase -
  RFF38_RS06860 (RFF38_06860) pilC 1419510..1420721 (-) 1212 WP_249984746.1 type II secretion system F family protein Machinery gene
  RFF38_RS06865 (RFF38_06865) pilB 1420721..1422109 (-) 1389 WP_115097850.1 GspE/PulE family protein Machinery gene
  RFF38_RS06870 (RFF38_06870) pilA 1422109..1422543 (-) 435 WP_115097851.1 prepilin peptidase-dependent pilin Machinery gene
  RFF38_RS06875 (RFF38_06875) ampD 1422703..1423269 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF38_RS06880 (RFF38_06880) rppH 1423855..1424451 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  RFF38_RS06885 (RFF38_06885) - 1424454..1425248 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  RFF38_RS06890 (RFF38_06890) lgt 1425258..1426061 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF38_RS06895 (RFF38_06895) - 1426067..1426918 (+) 852 WP_126417629.1 thymidylate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52091.68 Da        Isoelectric Point: 6.6308

>NTDB_id=875395 RFF38_RS06865 WP_115097850.1 1420721..1422109(-) (pilB) [Pasteurella multocida strain Past9]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPSKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRLQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=875395 RFF38_RS06865 WP_115097850.1 1420721..1422109(-) (pilB) [Pasteurella multocida strain Past9]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAACCTGTTTTGTTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCATCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGATATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCCGAGTTGGGGATG
ACCGCAGACCAACAACACAGGCTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-95)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.052

0.664
  pilB Haemophilus influenzae Rd KW20

67.108

98.052

0.658
  pilB Glaesserella parasuis strain SC1401

58.134

99.784

0.58
  pilB Vibrio parahaemolyticus RIMD 2210633

40.125

100

0.418
  pilB Acinetobacter baumannii D1279779

39.148

100

0.418
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.411
  pilB Vibrio cholerae strain A1552

38.683

100

0.407
  pilB Acinetobacter baylyi ADP1

37.475

100

0.405
  pilB Legionella pneumophila strain ERS1305867

36.605

100

0.387