Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RFF38_RS06870 Genome accession   NZ_CP133638
Coordinates   1422109..1422543 (-) Length   144 a.a.
NCBI ID   WP_115097851.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1417109..1427543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF38_RS06840 (RFF38_06840) - 1417670..1417939 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  RFF38_RS06845 (RFF38_06845) yacG 1417939..1418142 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  RFF38_RS06850 (RFF38_06850) coaE 1418153..1418773 (-) 621 WP_096743227.1 dephospho-CoA kinase -
  RFF38_RS06855 (RFF38_06855) - 1418785..1419510 (-) 726 WP_096743228.1 prepilin peptidase -
  RFF38_RS06860 (RFF38_06860) pilC 1419510..1420721 (-) 1212 WP_249984746.1 type II secretion system F family protein Machinery gene
  RFF38_RS06865 (RFF38_06865) pilB 1420721..1422109 (-) 1389 WP_115097850.1 GspE/PulE family protein Machinery gene
  RFF38_RS06870 (RFF38_06870) pilA 1422109..1422543 (-) 435 WP_115097851.1 prepilin peptidase-dependent pilin Machinery gene
  RFF38_RS06875 (RFF38_06875) ampD 1422703..1423269 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF38_RS06880 (RFF38_06880) rppH 1423855..1424451 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  RFF38_RS06885 (RFF38_06885) - 1424454..1425248 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  RFF38_RS06890 (RFF38_06890) lgt 1425258..1426061 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF38_RS06895 (RFF38_06895) - 1426067..1426918 (+) 852 WP_126417629.1 thymidylate synthase -
  RFF38_RS06900 (RFF38_06900) tadA 1426945..1427433 (+) 489 WP_108511809.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.51 Da        Isoelectric Point: 9.1281

>NTDB_id=875396 RFF38_RS06870 WP_115097851.1 1422109..1422543(-) (pilA) [Pasteurella multocida strain Past9]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=875396 RFF38_RS06870 WP_115097851.1 1422109..1422543(-) (pilA) [Pasteurella multocida strain Past9]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCTGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATTTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

35.333

100

0.368