Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RFF25_RS05940 Genome accession   NZ_CP133633
Coordinates   1270142..1271527 (-) Length   461 a.a.
NCBI ID   WP_083004806.1    Uniprot ID   -
Organism   Pasteurella multocida strain Pm1618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1265142..1276527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF25_RS05910 (RFF25_05905) - 1265269..1266642 (-) 1374 WP_083004820.1 alanine/glycine:cation symporter family protein -
  RFF25_RS05915 (RFF25_05910) - 1267091..1267360 (-) 270 WP_250190736.1 GNAT family N-acetyltransferase -
  RFF25_RS05920 (RFF25_05915) yacG 1267360..1267563 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  RFF25_RS05925 (RFF25_05920) coaE 1267574..1268194 (-) 621 WP_083004815.1 dephospho-CoA kinase -
  RFF25_RS05930 (RFF25_05925) - 1268206..1268931 (-) 726 WP_083004812.1 prepilin peptidase -
  RFF25_RS05935 (RFF25_05930) pilC 1268931..1270142 (-) 1212 WP_083004809.1 type II secretion system F family protein Machinery gene
  RFF25_RS05940 (RFF25_05935) pilB 1270142..1271527 (-) 1386 WP_083004806.1 GspE/PulE family protein Machinery gene
  RFF25_RS05945 (RFF25_05940) pilA 1271527..1271961 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  RFF25_RS05950 (RFF25_05945) ampD 1272121..1272687 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF25_RS05955 (RFF25_05950) rppH 1273273..1273869 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  RFF25_RS05960 (RFF25_05955) - 1273872..1274666 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  RFF25_RS05965 (RFF25_05960) lgt 1274676..1275479 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF25_RS05970 (RFF25_05965) - 1275485..1276336 (+) 852 WP_250190737.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52028.65 Da        Isoelectric Point: 6.7166

>NTDB_id=875327 RFF25_RS05940 WP_083004806.1 1270142..1271527(-) (pilB) [Pasteurella multocida strain Pm1618]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
KNELKRALQQLSPPKLQVEESIPTAYQVVQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQYRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGGFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=875327 RFF25_RS05940 WP_083004806.1 1270142..1271527(-) (pilB) [Pasteurella multocida strain Pm1618]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAGCCTGTTTTATTATCA
AAAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTAGAAGAAAGCATACCCACGGCTTATCA
AGTCGTGCAATTAGATTCTGATTCCGCAACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAACCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCGAAATTAGATATTAGTGA
AACACGCTTGCCTCAAGACGGCTGTTTTCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCAGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAATACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCGCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TACCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGGGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.351

100

0.584
  pilB Acinetobacter baumannii D1279779

39.394

100

0.423
  pilB Vibrio parahaemolyticus RIMD 2210633

40.125

100

0.419
  pilB Vibrio campbellii strain DS40M4

40.041

100

0.419
  pilB Vibrio cholerae strain A1552

39.463

100

0.414
  pilB Acinetobacter baylyi ADP1

37.675

100

0.408
  pilB Legionella pneumophila strain ERS1305867

36.81

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.243

91.757

0.36