Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RFF25_RS05945 Genome accession   NZ_CP133633
Coordinates   1271527..1271961 (-) Length   144 a.a.
NCBI ID   WP_083004804.1    Uniprot ID   A0A1Q1PRS7
Organism   Pasteurella multocida strain Pm1618     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1266527..1276961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF25_RS05915 (RFF25_05910) - 1267091..1267360 (-) 270 WP_250190736.1 GNAT family N-acetyltransferase -
  RFF25_RS05920 (RFF25_05915) yacG 1267360..1267563 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  RFF25_RS05925 (RFF25_05920) coaE 1267574..1268194 (-) 621 WP_083004815.1 dephospho-CoA kinase -
  RFF25_RS05930 (RFF25_05925) - 1268206..1268931 (-) 726 WP_083004812.1 prepilin peptidase -
  RFF25_RS05935 (RFF25_05930) pilC 1268931..1270142 (-) 1212 WP_083004809.1 type II secretion system F family protein Machinery gene
  RFF25_RS05940 (RFF25_05935) pilB 1270142..1271527 (-) 1386 WP_083004806.1 GspE/PulE family protein Machinery gene
  RFF25_RS05945 (RFF25_05940) pilA 1271527..1271961 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  RFF25_RS05950 (RFF25_05945) ampD 1272121..1272687 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RFF25_RS05955 (RFF25_05950) rppH 1273273..1273869 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  RFF25_RS05960 (RFF25_05955) - 1273872..1274666 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  RFF25_RS05965 (RFF25_05960) lgt 1274676..1275479 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  RFF25_RS05970 (RFF25_05965) - 1275485..1276336 (+) 852 WP_250190737.1 thymidylate synthase -
  RFF25_RS05975 (RFF25_05970) tadA 1276363..1276851 (+) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.46 Da        Isoelectric Point: 8.8040

>NTDB_id=875328 RFF25_RS05945 WP_083004804.1 1271527..1271961(-) (pilA) [Pasteurella multocida strain Pm1618]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=875328 RFF25_RS05945 WP_083004804.1 1271527..1271961(-) (pilA) [Pasteurella multocida strain Pm1618]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q1PRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

34.437

100

0.361