Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   RFB14_RS00145 Genome accession   NZ_CP133587
Coordinates   28290..28748 (+) Length   152 a.a.
NCBI ID   WP_003388831.1    Uniprot ID   M8E057
Organism   Brevibacillus borstelensis strain NEB264     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 23290..33748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFB14_RS00120 (RFB14_00120) - 23405..23494 (-) 90 WP_031932431.1 YjcZ family sporulation protein -
  RFB14_RS00125 (RFB14_00125) pepF 23845..25755 (+) 1911 WP_003388837.1 oligoendopeptidase F -
  RFB14_RS00130 (RFB14_00130) - 25809..26345 (-) 537 WP_024983209.1 GNAT family N-acetyltransferase -
  RFB14_RS00135 (RFB14_00135) ychF 26733..27833 (+) 1101 WP_003388835.1 redox-regulated ATPase YchF -
  RFB14_RS00140 (RFB14_00140) rpsF 27973..28260 (+) 288 WP_003388833.1 30S ribosomal protein S6 -
  RFB14_RS00145 (RFB14_00145) ssbA 28290..28748 (+) 459 WP_003388831.1 single-stranded DNA-binding protein Machinery gene
  RFB14_RS00150 (RFB14_00150) rpsR 28805..29035 (+) 231 WP_003388829.1 30S ribosomal protein S18 -
  RFB14_RS00155 (RFB14_00155) - 29142..30050 (+) 909 WP_003388828.1 YybS family protein -
  RFB14_RS00160 (RFB14_00160) - 30055..31992 (+) 1938 WP_003388827.1 DHH family phosphoesterase -
  RFB14_RS00165 (RFB14_00165) rplI 32023..32466 (+) 444 WP_003388826.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 16530.56 Da        Isoelectric Point: 5.2853

>NTDB_id=875157 RFB14_RS00145 WP_003388831.1 28290..28748(+) (ssbA) [Brevibacillus borstelensis strain NEB264]
MNKVILIGNLTKDPELRYTPNGVAVTTFTLAINRPRTNQAGEREADFINIVAWQKLADLCATYLRKGRQAAVEGRIQTRS
YDNKEGKKVYVTEVVAENVQFLGGKGGEGGGYDPGFGGMKPSGGQRDEMDAFGDPFANTGKPINISDDDLPF

Nucleotide


Download         Length: 459 bp        

>NTDB_id=875157 RFB14_RS00145 WP_003388831.1 28290..28748(+) (ssbA) [Brevibacillus borstelensis strain NEB264]
ATGAATAAAGTCATTCTCATCGGCAACCTGACAAAAGATCCGGAGCTGCGCTATACGCCAAACGGCGTGGCCGTTACTAC
CTTTACCCTGGCGATCAACCGTCCGCGTACCAACCAGGCAGGCGAACGCGAAGCCGACTTTATTAACATCGTCGCATGGC
AGAAGCTGGCTGACTTGTGCGCTACCTATCTGCGCAAAGGCAGACAAGCGGCTGTGGAGGGCCGTATTCAAACGCGCTCT
TATGATAATAAAGAAGGAAAAAAGGTTTACGTAACGGAAGTCGTCGCTGAAAACGTCCAGTTCTTGGGCGGAAAAGGCGG
AGAAGGCGGCGGTTACGATCCAGGCTTTGGCGGCATGAAGCCGTCGGGCGGTCAGAGAGATGAGATGGATGCGTTTGGGG
ATCCCTTTGCGAATACAGGCAAGCCGATCAACATCTCGGATGATGACTTGCCGTTTTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(1-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M8E057

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.605
  ssb Latilactobacillus sakei subsp. sakei 23K

43.787

100

0.487
  ssb Vibrio cholerae strain A1552

36.207

100

0.414
  ssb Neisseria gonorrhoeae MS11

36.047

100

0.408
  ssb Neisseria meningitidis MC58

35.465

100

0.401
  ssbB Bacillus subtilis subsp. subtilis str. 168

52.252

73.026

0.382
  ssb Glaesserella parasuis strain SC1401

31.638

100

0.368