Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RE395_RS04795 Genome accession   NZ_CP133579
Coordinates   1044087..1045124 (+) Length   345 a.a.
NCBI ID   WP_006422551.1    Uniprot ID   -
Organism   Stenotrophomonas geniculata strain G303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1039087..1050124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE395_RS04770 soxR 1040338..1040772 (+) 435 WP_019336938.1 redox-sensitive transcriptional activator SoxR -
  RE395_RS04775 - 1040846..1041448 (-) 603 WP_049410246.1 short chain dehydrogenase -
  RE395_RS04780 - 1041547..1042422 (+) 876 WP_049410249.1 LysR substrate-binding domain-containing protein -
  RE395_RS04785 proC 1042482..1043303 (-) 822 WP_049410252.1 pyrroline-5-carboxylate reductase -
  RE395_RS04790 - 1043322..1043999 (-) 678 WP_043398305.1 YggS family pyridoxal phosphate-dependent enzyme -
  RE395_RS04795 pilT 1044087..1045124 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  RE395_RS04800 pilU 1045272..1046366 (+) 1095 WP_049410297.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RE395_RS04805 - 1046488..1047471 (+) 984 WP_049481357.1 aldo/keto reductase -
  RE395_RS04810 - 1047514..1048410 (-) 897 WP_099560379.1 LysR substrate-binding domain-containing protein -
  RE395_RS04815 - 1048504..1048716 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  RE395_RS04820 - 1048858..1049262 (+) 405 WP_419965614.1 GNAT family N-acetyltransferase -
  RE395_RS04825 - 1049355..1050062 (+) 708 WP_029379885.1 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6652

>NTDB_id=875049 RE395_RS04795 WP_006422551.1 1044087..1045124(+) (pilT) [Stenotrophomonas geniculata strain G303]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=875049 RE395_RS04795 WP_006422551.1 1044087..1045124(+) (pilT) [Stenotrophomonas geniculata strain G303]
ATGGATATCGCCGAACTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGGCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCGTCGCTGGCGCGC
TTCCGTGTGAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGTTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGTGATACGCATGGTTTCAATGA
AGCGCTGCGCTCGGCACTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAACTTCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGTCGTACCGCTGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391