Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RE395_RS04800 Genome accession   NZ_CP133579
Coordinates   1045272..1046366 (+) Length   364 a.a.
NCBI ID   WP_049410297.1    Uniprot ID   -
Organism   Stenotrophomonas geniculata strain G303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040272..1051366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE395_RS04770 soxR 1040338..1040772 (+) 435 WP_019336938.1 redox-sensitive transcriptional activator SoxR -
  RE395_RS04775 - 1040846..1041448 (-) 603 WP_049410246.1 short chain dehydrogenase -
  RE395_RS04780 - 1041547..1042422 (+) 876 WP_049410249.1 LysR substrate-binding domain-containing protein -
  RE395_RS04785 proC 1042482..1043303 (-) 822 WP_049410252.1 pyrroline-5-carboxylate reductase -
  RE395_RS04790 - 1043322..1043999 (-) 678 WP_043398305.1 YggS family pyridoxal phosphate-dependent enzyme -
  RE395_RS04795 pilT 1044087..1045124 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  RE395_RS04800 pilU 1045272..1046366 (+) 1095 WP_049410297.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RE395_RS04805 - 1046488..1047471 (+) 984 WP_049481357.1 aldo/keto reductase -
  RE395_RS04810 - 1047514..1048410 (-) 897 WP_099560379.1 LysR substrate-binding domain-containing protein -
  RE395_RS04815 - 1048504..1048716 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  RE395_RS04820 - 1048858..1049262 (+) 405 WP_419965614.1 GNAT family N-acetyltransferase -
  RE395_RS04825 - 1049355..1050062 (+) 708 WP_029379885.1 YitT family protein -
  RE395_RS04830 - 1050148..1050693 (-) 546 WP_099560380.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40583.64 Da        Isoelectric Point: 6.7680

>NTDB_id=875050 RE395_RS04800 WP_049410297.1 1045272..1046366(+) (pilU) [Stenotrophomonas geniculata strain G303]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=875050 RE395_RS04800 WP_049410297.1 1045272..1046366(+) (pilU) [Stenotrophomonas geniculata strain G303]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTGCTCAACGTGATGACCCCTGCGCAGCGCGAGGAGT
TCGAGAAGACGCATGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCAATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTTGGTGCCACCGGTACCGGCAAATCGACATCGCTGGCGGCGA
TGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGTGAAGTCGGCATCGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACACTGCGCCAGGC
GCCGGACGTGATCATGATCGGCGAAGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTTTCGCTGAATCTGAAGGGCGTGGTTGCGCAGCAGCTGATTCCTTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATTCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGATGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAG
ATCAGCTACGAGGACGCACTGCGTTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCGCGTACCCTGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.436

98.352

0.673
  pilU Acinetobacter baylyi ADP1

64.368

95.604

0.615
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.923

89.286

0.365