Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   RCF60_RS11705 Genome accession   NZ_CP133320
Coordinates   2440314..2440751 (-) Length   145 a.a.
NCBI ID   WP_007612572.1    Uniprot ID   -
Organism   Bacillus velezensis strain 1X1Y     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2435314..2445751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF60_RS11655 (RCF60_11670) sinI 2435697..2435870 (+) 174 WP_032874029.1 anti-repressor SinI Regulator
  RCF60_RS11660 (RCF60_11675) sinR 2435904..2436239 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  RCF60_RS11665 (RCF60_11680) tasA 2436287..2437072 (-) 786 WP_032874027.1 biofilm matrix protein TasA -
  RCF60_RS11670 (RCF60_11685) sipW 2437137..2437721 (-) 585 WP_032874025.1 signal peptidase I SipW -
  RCF60_RS11675 (RCF60_11690) tapA 2437693..2438364 (-) 672 WP_032874023.1 amyloid fiber anchoring/assembly protein TapA -
  RCF60_RS11680 (RCF60_11695) - 2438623..2438952 (+) 330 WP_032874021.1 DUF3889 domain-containing protein -
  RCF60_RS11685 (RCF60_11700) - 2438993..2439172 (-) 180 WP_022552966.1 YqzE family protein -
  RCF60_RS11690 (RCF60_11705) comGG 2439229..2439606 (-) 378 WP_032874019.1 competence type IV pilus minor pilin ComGG Machinery gene
  RCF60_RS11695 (RCF60_11710) comGF 2439607..2440071 (-) 465 WP_223813077.1 competence type IV pilus minor pilin ComGF -
  RCF60_RS11700 (RCF60_11715) comGE 2440016..2440330 (-) 315 WP_032874016.1 competence type IV pilus minor pilin ComGE Machinery gene
  RCF60_RS11705 (RCF60_11720) comGD 2440314..2440751 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  RCF60_RS11710 (RCF60_11725) comGC 2440741..2441049 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  RCF60_RS11715 (RCF60_11730) comGB 2441054..2442091 (-) 1038 WP_032874014.1 competence type IV pilus assembly protein ComGB Machinery gene
  RCF60_RS11720 (RCF60_11735) comGA 2442078..2443148 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  RCF60_RS11725 (RCF60_11740) - 2443345..2444295 (-) 951 WP_032874012.1 magnesium transporter CorA family protein -
  RCF60_RS11730 (RCF60_11745) - 2444441..2445742 (+) 1302 WP_032874010.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16254.76 Da        Isoelectric Point: 10.1850

>NTDB_id=873435 RCF60_RS11705 WP_007612572.1 2440314..2440751(-) (comGD) [Bacillus velezensis strain 1X1Y]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIQLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=873435 RCF60_RS11705 WP_007612572.1 2440314..2440751(-) (comGD) [Bacillus velezensis strain 1X1Y]
TTGAACAATAACAGGCGGACAGAAAATGGGTTTACCCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTCCAAAAAGATA
TTCAGCTTGCTCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGAAGGATTGTCGAGCGTTCTTTTGATTCGCTTCATATTACACTTGTAACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCAATTGAAGAGCGCCGGGTTCACCTATGAGATAACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

55.479

100

0.559