Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WHU28_RS22565 Genome accession   NZ_CP148507
Coordinates   4392610..4393995 (+) Length   461 a.a.
NCBI ID   WP_063856362.1    Uniprot ID   -
Organism   Escherichia coli strain Z1323CEC0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4387610..4398995
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU28_RS22540 (WHU28_22530) aroP 4388158..4389531 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  WHU28_RS22545 (WHU28_22535) ampE 4389574..4390428 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  WHU28_RS22550 (WHU28_22540) ampD 4390425..4390976 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WHU28_RS22555 (WHU28_22545) nadC 4391064..4391957 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WHU28_RS22560 (WHU28_22550) pilA 4392160..4392600 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  WHU28_RS22565 (WHU28_22555) pilB 4392610..4393995 (+) 1386 WP_063856362.1 type II secretion system protein GspE Machinery gene
  WHU28_RS22570 (WHU28_22560) hofC 4393985..4395187 (+) 1203 WP_000157266.1 protein transport protein HofC -
  WHU28_RS22575 (WHU28_22565) guaC 4395222..4396265 (-) 1044 WP_001217337.1 GMP reductase -
  WHU28_RS22580 (WHU28_22570) - 4396421..4396465 (-) 45 WP_120795372.1 protein YacM -
  WHU28_RS22585 (WHU28_22575) coaE 4396490..4397110 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  WHU28_RS22590 (WHU28_22580) zapD 4397110..4397853 (+) 744 WP_001194721.1 cell division protein ZapD -
  WHU28_RS22595 (WHU28_22585) yacG 4397863..4398060 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  WHU28_RS22600 (WHU28_22590) mutT 4398276..4398665 (-) 390 WP_338930252.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50589.25 Da        Isoelectric Point: 6.6612

>NTDB_id=872887 WHU28_RS22565 WP_063856362.1 4392610..4393995(+) (pilB) [Escherichia coli strain Z1323CEC0001]
MNIPQLTALCLRYHGVSLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=872887 WHU28_RS22565 WP_063856362.1 4392610..4393995(+) (pilB) [Escherichia coli strain Z1323CEC0001]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTCGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.443

100

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

40.246

100

0.425
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

39.337

100

0.412
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Haemophilus influenzae Rd KW20

39.915

100

0.408
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Acinetobacter baumannii D1279779

43.655

85.466

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371