Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WHU28_RS22560 Genome accession   NZ_CP148507
Coordinates   4392160..4392600 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain Z1323CEC0001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4387160..4397600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHU28_RS22540 (WHU28_22530) aroP 4388158..4389531 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  WHU28_RS22545 (WHU28_22535) ampE 4389574..4390428 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  WHU28_RS22550 (WHU28_22540) ampD 4390425..4390976 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WHU28_RS22555 (WHU28_22545) nadC 4391064..4391957 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WHU28_RS22560 (WHU28_22550) pilA 4392160..4392600 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  WHU28_RS22565 (WHU28_22555) pilB 4392610..4393995 (+) 1386 WP_063856362.1 type II secretion system protein GspE Machinery gene
  WHU28_RS22570 (WHU28_22560) hofC 4393985..4395187 (+) 1203 WP_000157266.1 protein transport protein HofC -
  WHU28_RS22575 (WHU28_22565) guaC 4395222..4396265 (-) 1044 WP_001217337.1 GMP reductase -
  WHU28_RS22580 (WHU28_22570) - 4396421..4396465 (-) 45 WP_120795372.1 protein YacM -
  WHU28_RS22585 (WHU28_22575) coaE 4396490..4397110 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=872886 WHU28_RS22560 WP_000360904.1 4392160..4392600(+) (pilA) [Escherichia coli strain Z1323CEC0001]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=872886 WHU28_RS22560 WP_000360904.1 4392160..4392600(+) (pilA) [Escherichia coli strain Z1323CEC0001]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363